Effect of cycloheximide upon maturation of bovine oocytes

Simon, Markus M.; Jílek, F.; Fulka, Josef

doi:10.1051/rnd:19890502

Cited by 27 publications

(13 citation statements)

References 11 publications

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“…Both protein synthesis and protein phosphorylation are required during meiotic maturation of bovine oocytes (Sirard et al, 1989;Simon et al, 1989;Kastrop et al, 1990b;Fulka Jr et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Fertilisation competence of bovine normally matured or aged oocytes derived from different antral follicles: morphology, protein synthesis, H1 and MBP kinase activity

Pavlok

Kaláb

Bobák

1997

Zygote

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We have investigated the fertilisation competence, protein synthesis, histone HI kinase and myelin basic protein (MBP) kinase activities in three categories of bovine oocytes (derived from three size categories of follicles: M -medium, 2.5-5.0 mm; S -small, 1.5-2.5 mm; T -tiny, 1.0-1.5 mm). In contrast to more or less normal meiotic maturation (85.6%) and fertilisation (70.8%) of M oocytes cultured for 24 h, the fertilisation of M oocytes cultured for 40 h was associated with increased rates of retarded male pronuclear development and retention of the second polar body. The S and T oocytes cultured for 24 h or 40 h were mostly arrested at defective late diakinesis -metaphase I (77.5-100%) stage. After fertilisation of S and T oocytes cultured for 24 h no polar body was extruded and formation of one, three or four female pronuclei, together with mostly normal male pronuclei, was observed. The fertilisation of S and T oocytes after 40 h culture resulted in a higher number of female and a decreased number of male pronuclei. A major change in the pattern of protein synthesis was associated with the resumption of meiosis. There were no significant differences in the profile of protein synthesis between oocyte categories in all groups either matured or fertilised. The HI kinase activity reached comparable increased levels in oocytes of all categories matured for 24 h and decreased during the 40 h culture, most significantly in M oocytes. The MBP kinase activity was at approximately the same high level in all categories of oocytes after 24 h of culture and remained stable until 40 h. The fertilisation after 24 h of culture resulted, in M oocytes, in low levels of both HI and MBP kinase activities; in S oocytes, only HI kinase was completely inactivated while MBP kinase activity decreased to some extent; in T oocytes, both HI and MBP kinase activity decreased. Fertilisation of all oocyte categories after 40 h culture resulted in complete inactivarion of both these kinases to their basal levels.

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Section: Introductionmentioning

confidence: 99%

Fertilisation competence of bovine normally matured or aged oocytes derived from different antral follicles: morphology, protein synthesis, H1 and MBP kinase activity

Pavlok

Kaláb

Bobák

1997

Zygote

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“…Mouse oocytes are able to induce GVBD in the presence of protein synthesis inhibitor (cycloheximide; CX) (Fulka et al, 1986;Motlik and Rimkevicova, 1990;Szollosi et al, 1991), whereas CX completely blocks GVBD in domestic animals (pig, Fulka et al, 1986;Kubelka et al, 1988;goat, Le Gal et al, 1992; cattle, Hunter and Moor, 1987;Tatemoto et al, in press). Simon et al (1989) and Kastrop et al (1991) observed that the addition of CX after various periods of culture resulted in an increasing percentage of GVBD in bovine oocytes, suggesting that protein synthesis after the onset of culture was necessary for the initiation of GVBD. Such protein synthesis probably involves the activation of maturation-promoting factor (MPF), although the correlation between them is still not clear in bovine oocytes.…”

Section: Introductionmentioning

confidence: 99%

Requirement for protein synthesis during the onset of meiosis in bovine oocytes and its involvement in the autocatalytic amplification of maturation‐promoting factor

Tatemoto

Horiuchi

1995

Molecular Reproduction Devel

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The present study was carried out using the method of electrofusion, or treatment with okadaic acid (OA), to determine whether protein synthesis at the onset of culture was required for the meiotic resumption of bovine follicular oocytes. Germinal vesicle breakdown (GVBD) occurred in bovine oocytes at 6 hr after separation from their follicles in vitro. Following this, immature germinal vesicle (GV) oocytes, preincubated for 0, 2, 4, and 6 hr, were fused to mature oocytes. When immature oocytes, preincubated for 0 hr, were fused to mature oocytes and then cultured for 3 hr in basic medium, GVBD was observed in all fused cells, whereas in the case of cultivation in medium supplemented with the protein synthesis inhibitor (25 micrograms/ml cycloheximide; CX), 39% of the fused cells possessed an intact GV within their cytoplasm. In immature oocytes preincubated for 4 or 6 hr, however, this proportion was significantly reduced to 7% and 4%, respectively, without protein synthesis after fusion. In addition, the CX-dependent block of GVBD could be overcome in only 13% of bovine follicular oocytes by the addition of 2 microM OA, although 51% of oocytes which synthesized the protein during the first 6 hr of culture induced GVBD in subsequent culture with CX plus OA. Thus, we conclude that the initiation of GVBD in bovine oocytes requires protein synthesized at the onset of meiosis, which is related to the autocatalytic amplification of the maturation-promoting factor.

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“…However, problems remain with subsequent development of oocytes after IVF such as a low incidence and quality of blastocysts. In the methods for inhibiting Accepted for publication: December 17, 2001 Correspondence: K. Imai t h e m e i o s i s o f o o c y t e s u s i n g c h e m i c a l s , cycloheximide [9,10] and 6-dimethylaminopurine [11,12] are well-known germinal vesicles break down (GVBD) inhibitors. However, their effects on subsequent maturation and developmental competence after IVF of bovine oocytes are unsatisfactory.…”

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confidence: 99%

Effects of Butyrolactone-I on GVBD in Bovine Oocytes and Subsequent Maturation, Fertilization and Development In Vitro.

Imai

Kobayashi

Kaneyama

et al. 2002

Journal of Reproduction and Development

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Abstract. In Experiment 1, bovine cumulus oocyte complexes (COCs) were preincubated for 24 h in M199 supplemented with 25-100 µM BL-I, an inhibitor of cdc2 kinase activity. Half of the COCs were fixed to assess rates of germinal vesicle breakdown (GVBD), and the rest were cultured for a further 20 h without BL-I to investigate whether BL-1 affects their meiotic competence. In Experiment 2, COCs treated with BL-I for 24 h or 48 h were matured (IVM), fertilized (IVF) and then cultured in vitro (IVC) for 8 days. Oocytes exposed to 100 µM BL-I showed a significantly higher rate of germinal vesicle (GV) (86%) stage than the others (4-72%). Oocytes exposed to 100 µM BL-I for 48 h showed equally high GV (95%) stage and maturation (84%) rates compared to oocytes treated for 24 h and control oocytes with no treatment. However, they showed significantly lower normal fertilization (27%), cleavage (27%) and blastocyst formation (6%) rates than oocytes treated for 24 h (66, 69 and 42 %, respectively) and control oocytes (65, 75 and 42%, respectively). The results indicate that BL-I can reversibly inhibit GVBD of bovine oocytes for at least 24 h but not for 48 h without compromising subsequent developmental competence after IVM-IVF-IVC.

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Effect of cycloheximide upon maturation of bovine oocytes

Cited by 27 publications

References 11 publications

Fertilisation competence of bovine normally matured or aged oocytes derived from different antral follicles: morphology, protein synthesis, H1 and MBP kinase activity

Fertilisation competence of bovine normally matured or aged oocytes derived from different antral follicles: morphology, protein synthesis, H1 and MBP kinase activity

Requirement for protein synthesis during the onset of meiosis in bovine oocytes and its involvement in the autocatalytic amplification of maturation‐promoting factor

Effects of Butyrolactone-I on GVBD in Bovine Oocytes and Subsequent Maturation, Fertilization and Development In Vitro.

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