Effect of cytokinins on expression of radish CLE genes

Додуева, И. Е.; Kiryushkin, A. S.; Yurlova, E. V.; Osipova, M. A.; Buzovkina, I. S.; Лутова, Л. А.

doi:10.1134/s1021443713020052

Cited by 10 publications

(7 citation statements)

References 22 publications

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“…Cycle threshold values were obtained with the accompanying CFX manager software, and data were analyzed by the 2 −ΔΔCt method [ 120 ]. The relative expression was normalized against constitutively expressed R. sativus ubiquitin ( RsUBQ11 ) and glyceraldehyde-3-phosphate dehydrogenase ( RsGAPDH ) genes [ 121 ]. Experiments were repeated three times with independent biological samples, whose results were then averaged.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic Analysis of Radish (Raphanus sativus L.) Spontaneous Tumor

Tkachenko

Додуева

Tvorogova

et al. 2021

Plants

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Spontaneous tumors can develop in different organs of various plant species without any pathogen infection and, as a rule, appear in plants with a certain genotype: Mutants, interspecific hybrids, etc. In particular, among the inbred lines of radish (Raphanus sativus L.), lines that form spontaneous tumors on the taproot during the flowering period were obtained many years ago. In this work, we analyzed the differential gene expression in the spontaneous tumors of radish versus the lateral roots using the RNA-seq method. Data were obtained indicating the increased expression of genes associated with cell division and growth (especially genes that regulate G2-M transition and cytokinesis) in the spontaneous tumor. Among genes downregulated in the tumor tissue, genes participating in the response to stress and wounding, mainly involved in the biosynthesis of jasmonic acid and glucosinolates, were enriched. Our data will help elucidate the mechanisms of spontaneous tumor development in higher plants.

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Section: Methodsmentioning

confidence: 99%

Transcriptomic Analysis of Radish (Raphanus sativus L.) Spontaneous Tumor

Tkachenko

Додуева

Tvorogova

et al. 2021

Plants

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“…The RNA pellet was washed three (Livak & Schmittgen, 2001). Relative expression was normalised against constitutively expressed R. sativus ubiquitin (RsUBQ11) and glyceraldehyde-3-phosphate dehydrogenase (RsGAPDH) genes (Dodueva et al, 2013).…”

Section: Qpcrmentioning

confidence: 99%

Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines

Tkachenko

Gancheva

Tvorogova

et al. 2021

Annals of Applied Biology

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Studying aberrations of normal developmental processes like plant tumour initiation provides a great opportunity for understanding of systemic control of plant cells differentiation. Most well-studied example of plant tumour is the crown gall caused by Agrobacterium tumefaciens; much less studied are spontaneous tumours developing in plants with certain genotype, regardless of agrobacterial infection. In the present study, we used RNA-seq to analyse the changes in gene expression at the early stages of development of A. tumefaciens-induced tumours in radish seedlings. In our studies we used two closely related radish inbred lines contrasting in their ability to form spontaneous tumours. RNA-seq and qPCR analysis showed significant changes in the expression levels for numerous genes involved in different metabolic and developmental processes, and Gene Ontology analysis indicated many biological processes probably involved in the crown gall development in radish. For example, a unique result of our research is upregulation of genes related to photomorphogenesis and downregulation of senescence-associated genes in the young crown gall tumours. Differences in gene expression between the analysed radish lines included upregulation of pathways related to chromatin remodelling and protein targeting to chloroplast in the tumorous line along with downregulation of pathways of chaperone-mediated complex assembly and anthocyanin biosynthesis.

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“…Analysis of the expression of identified radish CLE genes in radish seedlings in response to different periods of treatment with exogenous cytokinins and auxins showed that cytokinins suppress the expression of CLE genes encoding peptides of group A (negative regulators of meristem maintenance) and do not affect the expression of CLE genes of group B (cambium proliferation activators). At the same time, auxins suppress the expression of CLE genes of group B and activate the expression of some CLE genes of group A, such as CLE19 (Dodueva et al, 2013;Gancheva et al, 2016). Thus, the action of auxins and cytokinins in the development of spontaneous tumors in radish lines can be mediated through the WOX-CLAVATA system, of which CLE peptides are a component.…”

Section: Geneticsmentioning

confidence: 99%

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

Lutova

Додуева

2019

BioComm

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This review highlights the years of research on the genetics of in vitro regeneration in higher plants conducted at the Department of Genetics and Biotechnology of Saint Petersburg State University. The genetic collection of radish (Raphanus sativus) created at the department by selfing of individual plants from three cultivars was used as a model in these studies. Some radish inbred lines from the genetic collection form spontaneous tumors in the roots and are also used to study mechanisms of tumor growth in higher plants. It was revealed that radish lines that differed in the ability to form tumors also contrastingly differed in the reaction of their explants to auxin and cytokinin in vitro, which reflects a difference in the levels of these hormones in the tissues of related tumorous and non-tumorous radish lines. Moreover, high concentrations of cytokinins in cultural medium induced tumor formation in the regenerated plants of tumorous radish lines. The presence of meristematic zones in spontaneous tumors in radish lines, as well as in crown gall tumors induced by Agrobacterium tumefaciens and cytokinin-induced tumors made it possible to reveal the role of the main meristem regulators, such as KNOX and WOX family transcription factors and the CLAVATA system, in both the process of tumor growth and regeneration in plants. Analysis of the expression of meristem-specific genes during the development of spontaneous and induced tumors in radish as well as in regenerated radish plants confirmed this assumption.

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Effect of cytokinins on expression of radish CLE genes

Cited by 10 publications

References 22 publications

Transcriptomic Analysis of Radish (Raphanus sativus L.) Spontaneous Tumor

Transcriptomic Analysis of Radish (Raphanus sativus L.) Spontaneous Tumor

Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines

Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype

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