Effect of Delta Sleep-Inducing Peptide on Functional State of Hepatocytes in Rats During Restraint Stress

Abstract

We studied the effect of delta sleep-inducing peptide (40, 120, and 360 μg/kg intraperitoneally, 1 h before the experiment) on free radical oxidation in the liver, aminotransferase activity, and total serum protein content in male Wistar rats during restraint stress. Treatment with the peptide in a dose of 40 μg/kg increased catalase and superoxide dismutase (SOD) activities and malonic dialdehyde (MDA) concentration in the liver homogenate of animals subjected to acute stress. No significant changes were found after administration of this peptide in other doses. Under conditions of chronic stress, the peptide in a dose of 40 μg/kg caused the most pronounced effect. Catalase and SOD activities and MDA concentration decreased, while aminotransferase activity and protein content remained unchanged under these conditions. Administration of the peptide in a dose of 120 μg/kg was accompanied by a decrease in SOD activity and MDA concentration, increase in total protein content, and reduction of AST activity. Increasing the peptide dose to 360 μg/kg abolished its effects.

Modulatory effects of delta sleep-inducing peptide in a lindane model of generalized seizures

“…Epileptogenesis is also a consequence of oxidative stress [39,40]. DSIP optimizes the prooxidantantioxidant balance by increasing the activities of antioxidant enzymes and the levels of antioxidants [41,42]. These effects of DSIP on antioxidant capacities could be explained by increased expression of genes for superoxide dismutase 1 and glutathione peroxidase 1, which were evoked by prolonged DSIP treatment [43].…”