Contamination of resilient denture liner material with microorganisms, particularly Candida albicans, is a common clinical problem. Denture hygiene is essential to maintain the serviceability of the denture, and microwave has been suggested for denture disinfection. A resilient denture liner material was contaminated with Candida albicans and reduction of organism counts after test disinfection methods (microwave energy, soaking overnight in a dilute sodium hypochlorite and alkaline peroxide solutions) calculated.