Effect of deproteinization methods on the antioxidant activity of polysaccharides extracted from Lentinus edodes stipe

Shunfeng, Li; Wang, Anjian; Liu, Lina; Gao, Tian; Xu, Fangfang

doi:10.1007/s11694-019-00054-2

Cited by 28 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of uronic acid has been confirmed once again with the chemical shift signal at δ 170.66 ppm. Together with previous studies, our findings suggest: (1) the 1 H signal at δ 4.99 ppm and the 13 C signal at δ 107.44 ppm demonstrated the existence of β-D-Gal in BCP [ 54 ]; (2) The 1 H signal at approximately δ 4.38 ppm and the 13 C signal around δ 107.08 ppm were due to the existence of β-L-Rha. Besides, the 1 H signal at δ 5.04 ppm and the 13 C signal at δ 103.40 ppm demonstrated the presence of α-L-Araf [ 55 ].…”

Section: Resultssupporting

confidence: 85%

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

et al. 2022

View full text Add to dashboard Cite

This study explored the preliminary structural characteristics and in vivo anti-tumor activity of an acidic water-soluble polysaccharide (BCP) separated purified from Bupleurum chinense DC root. The preliminary structural characterization of BCP was established using UV, HPGPC, FT-IR, IC, NMR, SEM, and Congo red. The results showed BCP as an acidic polysaccharide with an average molecular weight of 2.01 × 103 kDa. Furthermore, we showed that BCP consists of rhamnose, arabinose, galactose, glucose, and galacturonic acid (with a molar ratio of 0.063:0.788:0.841:1:0.196) in both α- and β-type configurations. Using the H22 tumor-bearing mouse model, we assessed the anti-tumor activity of BCP in vivo. The results revealed the inhibitory effects of BCP on H22 tumor growth and the protective actions against tissue damage of thymus and spleen in mice. In addition, the JC-1 FITC-AnnexinV/PI staining and cell cycle analysis have collectively shown that BCP is sufficient to induce apoptosis and of H22 hepatocarcinoma cells in a dose-dependent manner. The inhibitory effect of BCP on tumor growth was likely attributable to the S phase arrest. Overall, our study presented significant anti-liver cancer profiles of BCP and its promising therapeutic potential as a safe and effective anti-tumor natural agent.

show abstract

Section: Resultssupporting

confidence: 85%

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Although the Sevag method was used to deproteinize the LECPs, the protein content detected was 3.41%, suggesting that it could be a protein-bound polysaccharide. It has been reported that the antioxidant activity of protein-bound polysaccharides is enhanced by a synergistic effect [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…The EC 50 value of LECP (0.51 ± 0.05 mg/mL) was lower compared to that reported for Lentinus edodes (2.16 mg/mL) [ 9 ], Pleurotus ostreatus (2.36 mg/mL) [ 46 ] and Pleurotus djamor (3.83 g/mL) [ 47 ] polysaccharides, indicating the great potential of LECP to scavenge DPPH radicals. This enhanced activity of LECP could be related to the presence of proteins, since it has been shown that there is a synergistic effect in the ability to eliminate the DPPH radical between polysaccharides and proteins [ 33 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power

2022

View full text Add to dashboard Cite

The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides. In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated. The antioxidant activity of LECP was investigated against reactive species such as 1,1’-diphenyl-2-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively. Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde. The main sugar composition of LECP includes mannose, galactose, glucose, fucose and glucuronic acid. Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues. The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation. The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.

show abstract

“…More recently, LBP-P was found to enhance host immunity (Chen et al, 2009) and inhibit the proliferation and migration of BIU87 cells as a therapeutic strategy for bladder cancer treatment. Many deproteinization methods have been developed to try to remove protein impurities of polysaccharides (Cheng et al, 2019;Li et al, 2019;Shi et al, 2019;Song et al, 2019;Duan et al, 2020) (Table 1). Among these methods, the Sevag method was a typical and traditional deproteinization technology for the purification of polysaccharides in Lycium barbarum berries (Chen et al, 2008;Yang and Zhang, 2009;Zhu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Box-Behnken design-based optimization for deproteinization of crude polysaccharides in Lycium barbarum berry residue using the Sevag method

Long

Qing

cai

et al. 2020

Heliyon

View full text Add to dashboard Cite

Deproteinization of crude polysaccharides in the residue from Lycium barbarum berries (LBBs) was conducted using the Sevag method. A Box-Behnken design based on single-factor experiments was employed to optimize the deproteinization technology. The results showed that the deproteinization conditions had significant effects on the extraction yield of polysaccharides and the residual protein content in Lycium barbarum polysaccharides (LBP). The experimental data were fitted to a second-order polynomial equation, using multiple regression analysis with a high coefficient of determination (R 2 ) value. The optimal conditions were as follows: a ratio of raw material to water extract concentrate from the residual LBBs of 0.15 g/mL; a ratio of chloroform to n-butyl alcohol of 2.17 mL/mL; and a ratio of water extract concentrate from residual LBBs to Sevag reagent of 0.50 mL/mL; with a maximum polysaccharide yield of 0.49% and minimum residual protein content of 0.087%. The results were confirmed through validation experiments. GPC analysis indicated that deproteinized LBP molecules became much more homogeneous. X-ray diffraction indicated that the hydrogen bonding of deproteinized LBP was weakened. This optimization of LBP should be a useful method for purification of crude LBP.

show abstract

Effect of deproteinization methods on the antioxidant activity of polysaccharides extracted from Lentinus edodes stipe

Cited by 28 publications

References 16 publications

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

The Structural Characteristics of an Acidic Water-Soluble Polysaccharide from Bupleurum chinense DC and Its In Vivo Anti-Tumor Activity on H22 Tumor-Bearing Mice

Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power

Box-Behnken design-based optimization for deproteinization of crude polysaccharides in Lycium barbarum berry residue using the Sevag method

Contact Info

Product

Resources

About