Effect of desensitising paste containing 8% arginine and calcium carbonate on biofilm formation of Streptococcus mutans in vitro

Fu, Donglei; Pei, Dandan; Huang, Cui; Liu, Yinchen; Du, Xianjie; Sun, Hairong

doi:10.1016/j.jdent.2013.04.013

Cited by 21 publications

(17 citation statements)

References 32 publications

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“…A previous study (22) showed that desensitizing paste containing 8% arginine and calcium carbonate (Ar-Ca) onto hypersensitive surfaces of teeth can significantly suppress S. mutans biofilm formation and maturation, which is consistent with our findings.…”

Section: Discussionsupporting

confidence: 93%

Are Desensitizing Toothpastes Equally Biocompatible and Effective Against Microorganisms?

et al. 2017

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The aims of this study were evaluate cytotoxicity, genotoxicity, antimicrobial activity of desensitizing toothpastes compared to a common one and the surface roughness of tooth enamel submitted to brushing with these toothpastes. Samples of three desensitizing toothpastes (Colgate Sensitive, Sensodyne and Oral B Sensitive) and common toothpaste (Colgate) were placed in contact with gingival human fibroblasts. Cytotoxicity and genotoxocity were measured by MTT assay and micronucleus test. Antimicrobial activity of the toothpastes extracts against C. albicans, S. mutans and S. aureus were assessed. For surface roughness evaluation, bovine teeth were submitted to 10.000 brushing cycles. The results were analyzed statically using Mann-Whitney U, ANOVA and Z tests (p<0.05). All toothpastes caused cytotoxic effect to the cells (p<0.05), except Colgate Sensitive. The toothpastes did not increase the number of micronuclei compared to the untreated control group. Colgate eliminated all the evaluated microorganisms at lower concentrations compared to Colgate Sensitive and Oral B Sensitive, which were not able to eliminate S. aureus. Sensodyne did not reach the minimum microbicidal concentration. The surface roughness of tooth enamel increased after brushing with Colgate Sensitive and Oral B Sensitive, however the comparison between groups showed no difference on the enamel surface roughness presented by desensitizing toothpastes when compared with the common one (p>0.05). Based on these results, we can conclude that although none toothpaste has induced genotoxicity, Colgate Sensitive was also not cytotoxic. Colgate was the most effective against the microorganisms, and there were no differences on the enamel surface roughness between the groups.

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Section: Discussionsupporting

confidence: 93%

Are Desensitizing Toothpastes Equally Biocompatible and Effective Against Microorganisms?

et al. 2017

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“…S. mutans growth is an essential process in dental caries development. 16 Moreover, S. mutans plays a key role in the development of oral biofilms through the production of extracellular polysaccharides. S. mutans is a major target for the prevention of dental caries; thus, we chose S. mutans as an antibacterial objective in our research.…”

Section: Discussionmentioning

confidence: 99%

“…According to the modified method 16 and the results of the preliminary experiment with a series of tenfold dilution, CNE and CHX were individually diluted to 1, 2, 4, 8, and 16 μg/mL of CNE with distilled water. Then, 0.2 mL solutions were individually added to 1.8 mL of BHI bacterial suspension (10 7 CFU/mL) and incubated anaerobically (Thermo 3427; Thermo Scientific, Waltham, MA, USA) in 24-well plates (Costar 3524; Corning Inc., Corning, NY, USA) at 37°C for 24 hours, with shaking at 200× g. After incubation, 200 μL samples from each well were moved to a 96-well plate and tested using an enzyme-linked immunosorbent reader (BioRad 6.0; Bio-Rad Laboratories Inc., Hercules, CA, USA) at 595 nm.…”

Section: Determination Of Mic and Mbcmentioning

confidence: 99%

Inhibited biofilm formation and improved antibacterial activity of a novel nanoemulsion against cariogenic Streptococcus mutans in vitro and in vivo

Li¹,

Sun²,

Gao³

et al. 2015

IJN

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The aim of this study was to prepare a novel nanoemulsion loaded with poorly water-soluble chlorhexidine acetate (CNE) to improve its solubility, and specifically enhance the antimicrobial activity against Streptococcus mutans in vitro and in vivo . In this study, a novel CNE nanoemulsion with an average size of 63.13 nm and zeta potential of −67.13 mV comprising 0.5% CNE, 19.2% Tween 80, 4.8% propylene glycol, and 6% isopropyl myristate was prepared by the phase inversion method. Important characteristics such as the content, size, zeta potential, and pH value of CNE did not change markedly, stored at room temperature for 1 year. Also, compared with chlorhexidine acetate water solution (CHX), the release profile results show that the CNE has visibly delayed releasing effect in both phosphate-buffered saline and artificial saliva solutions ( P <0.005). The minimum inhibitory concentration and minimum bactericidal concentration of CHX for S. mutans (both 0.8 μg/mL) are both two times those of CNE (0.4 μg/mL). Besides, CNE of 0.8 μg/mL exhibited fast-acting bactericidal efficacy against S. mutans , causing 95.07% death within 5 minutes, compared to CHX (73.33%) ( P <0.01). We observed that 5 mg/mL and 2 mg/mL CNE were both superior to CHX, significantly reducing oral S. mutans numbers and reducing the severity of carious lesions in Sprague Dawley rats ( P <0.05), in an in vivo test. CNE treatment at a concentration of 0.2 μg/mL inhibited biofilm formation more effectively than CHX, as indicated by the crystal violet staining method, scanning electron microscopy, and atomic force microscopy. The cell membrane of S. mutans was also severely disrupted by 0.2 μg/mL CNE, as indicated by transmission electron microscopy. These results demonstrated that CNE greatly improved the solubility and antimicrobial activity of this agent against S. mutans both in vitro and in vivo. This novel nanoemulsion is a promising medicine for preventing and curing dental caries.

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“…Samples in the AMS then were put in an incubator at 37° C. The images of the restoration material surface of RK were analyzed using SEM, a modification of procedures conducted by Fu. 9 Media in microtube and AMS were discarded, and the surface of RK was washed using PBS to clean up the remaining BHI broth attached to the surface. One of RK samples from each group was fixed with 4% paraformaldehid solution with a pH of 7.4 for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

Differences of Streptococcus mutans adhesion between artificial mouth systems: a dinamic and static methods

Morita¹,

Yulianto²,

Kusdina³

et al. 2017

Dent. J. (Majalah Kedokteran Gigi)

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Background: Various materials have been used for treating dental caries. Dental caries is a disease that attacks hard tissues of the teeth. The initial phase of caries is a formation of bacterial biofilm, called as dental plaque. Dental restorative materials are expected for preventing secondary caries formation initiated by dental plaque. Initial bacterial adhesion is assumed to be an important stage of dental plaque formation. Bacteria that recognize the receptor for binding to the pellicle on tooth surface are known as initial bacterial colonies. One of the bacteria that plays a role in the early stage of dental plaque formation is Streptococcus mutans (S. mutans). Artificial mouth system (AMS) used in bacterial biofilm research on the oral cavity provides the real condition of oral cavity and continous and intermittent supply of nutrients for bacteria. Purpose: This study aimed to compare the profile of S. mutans bacterial adhesion as the primary etiologic agent for dental caries between using static method and using artificial mouth system, a dinamic. method (AMS). Method: The study was conducted at Faculty of Dentistry and Integrated Research and testing laboratory (LPPT) in Universitas Gadjah Mada from April to August 2015. Composite resin was used as the subject of this research. Twelve composite resins with a diameter of 5 mm and a width of 2 mm were divided into two groups, namely group using static method and group using dynamic method. Static method was performed by submerging the samples into a 100µl suspension of 1.5 x 108 CFU/ml S. mutans and 200µl BHI broth. Meanwhile AMS method was carried out by placing the samples at the AMS tube drained with 20 drops/minute of bacterial suspension and sterile aquadest. After 72 hours, five samples from each group were calculated for their biofilm mass using 1% crystal violet and read by a spectrofotometer with a wavelength of 570 nm. Meanwhile, one sample from each group was taken for its surface image using Scanning Electron Microscope (SEM). Result: The results showed that S. mutans biofilm mass in the group using static method was 0.34, while in the group using AMS method was 0.09. The results of the statistical analysis then showed that there was a significant difference (p=0.02) in the formation of bacterial biofilm mass between those groups. SEM image in the group using static method also showed that the attachment of S. mutans was more numerous and had a longer chain than in the group using AMS method. Conclusion: There is a difference in the profile of S. mutans bacterial adhesion between using AMS method and static method.

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Effect of desensitising paste containing 8% arginine and calcium carbonate on biofilm formation of Streptococcus mutans in vitro

Cited by 21 publications

References 32 publications

Are Desensitizing Toothpastes Equally Biocompatible and Effective Against Microorganisms?

Are Desensitizing Toothpastes Equally Biocompatible and Effective Against Microorganisms?

Inhibited biofilm formation and improved antibacterial activity of a novel nanoemulsion against cariogenic Streptococcus mutans in vitro and in vivo

Differences of Streptococcus mutans adhesion between artificial mouth systems: a dinamic and static methods

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