Effect of Developmental Stage and Medium on Embryo Culture of Low Chill Peach Hybrids

Devi, Indira; Singh, Harminder; Thakur, Anirudh

doi:10.18520/cs/v113/i09/1771-1775

Cited by 11 publications

(8 citation statements)

References 6 publications

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“…This difference could be due to the environment and cultural conditions which were not the same. The results obtained are similar to those of Devi et al (2017) who, using the control medium, obtained germination rate (73%) during the in vitro culture of mature zygotic embryos of Prunus persica.…”

Section: Resultssupporting

confidence: 88%

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Kamdem

Donfagsiteli²,

Mache

et al. 2022

Plant Tissue Cult. & Biotech.

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Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida. Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)

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Section: Resultssupporting

confidence: 88%

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Kamdem

Donfagsiteli²,

Mache

et al. 2022

Plant Tissue Cult. & Biotech.

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“…Hybrid embryos 'Summerglo' × 'Nikitskiy Podarok' and 'Loadel' × 'Nikitskiy Podarok' were characterized by high regeneration capacity (96.30 and 92.59%, respectively). These data correspond to those by Monnier [58], Bridgen [59] and Devi et al [50] that in the mature embryos culture, PGRs are usually not used or are used in low concentrations, since high concentrations of PGRs reduce the germination rate of peach embryos. Some obtained seedlings of four peach cross combinations for morphogenic capacity investigation were used.…”

Section: Discussionsupporting

confidence: 90%

“…Many researchers have reported t the frequency of plantlet in vitro regeneration depends on the effect of various fact [40][41][42][43][44][45][46][47]. The ability of peach and other stone fruit crops embryos to form normal pla lets depends, to a great extent, on the exposure to a low positive temperature of 4-5 without light [30,[48][49][50]. In our experiments, the absence of cold pretreatment resulted abnormal development of seedlings in all cross combinations with undeveloped ro Daorden et al [51] have reported that stratification and in vitro culture made it poss to shorten the time of peach embryo germination and obtain 91% of developed pla from seeds stratified at 4 °C.…”

Section: Discussionmentioning

confidence: 99%

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

et al. 2021

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Peach [Prunus persica (L.) Batsch] is among the most demanded fruit crops in the world. Biotechnological methods help to originate new hybrid forms in order to increase the cultivar diversity and create new valuable genotypes. Cross combinations between the cultivars Clyde Wilson, Jerseyglo, Loadel, Summerglo and the promising cultivar ‘Nikitskiy Podarok’ have been done. The embryos of these hybrids germinated and formed plantlets after stratification at 4 °C for 45–60 days. The best regeneration rates in the hybrids ‘Loadel’ × ‘Nikitskiy Podarok’ and ‘Summerglo’ × ‘Nikitskiy Podarok’ (96.30% and 92.59%, respectively) were noted on hormone-free Monnier culture medium supplemented with 400.0 mg L−1 casein hydrolyzate. When the newly formed plantlets had necrosis of the shoot apex or immature roots, nodal shoot segments were used. At the same time, a high regeneration capacity was noted in the hybrids ‘Summerglo’ × ‘Nikitskiy Podarok’ and ‘Loadel’ × ‘Nikitskiy Podarok’ on B5 culture medium with 0.75 mg L−1 6–benzyl–aminopurine (BAP) + 0.1 mg L−1 indole–3–butyric acid (IBA). After the second subculture, the number of new adventitious shoots was 5.18 ± 0.18 and 4.95 ± 0.18 shoots per explant, respectively. The plants obtained from the hybrid embryos in a soil mixture soil: peat: sand (3:1:1) were adapted. The main morphological and anatomical features of the leaf blades in newly originated peach hybrids have been studied: the thickness of their tissues and the distribution of stomatal apparatus, as well as the physiological parameters of the photosystem II activity in regenerants cultured in vitro and during their in vivo acclimatization. The high capacity to post aseptic adaptation in the obtained hybrids has been shown.

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“…As the use for reproductive purposes is hampered by the low or null seed germination, it is necessary to rescue embryos in vitro (SUNDOURI, 2014). Embryo culture provides ideal conditions for the immature zygotic embryo to complete its development cycle in aseptic medium (DEVI et al, 2017). Several nutritional media have been used in the in vitro rescue of Prunus embryos, most of which based on MS medium and its variations (GERCHEVA et al, 2002;LIU et al, 2007;REIS et al, 2012;SUNDOURI et al, 2014;MANSVELT et al, 2015;DEVI et al, 2017) and WPM (RAMMING et al, 2003;EINHARDT, 2010;NASCIMENTO et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

et al. 2020

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Early-ripening peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative, in which seeds find conditions to complete germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but they still have problems and need to be optimized. The objective of this work was to test culture media and BAP concentrations in order to increase the germination percentage of embryos and viable ‘BRS Kampai’ peach seedlings, avoiding possible anomalies in the process. The experiment was carried out at the Laboratory of Tissue Culture – “Universidade Tecnológica Federal do Paraná”, Campus of Pato Branco. The experimental design used was randomized blocks, in 2x5 factorial, with four replicates, with plot being represented by 15 embryos. The culture media tested were MS and WPM with five BAP concentrations (0, 1, 2, 3 and 4 mg L-1). In the in vitro period, seed germination was evaluated through the attribution of scores, and embryo development, measuring the length of stem and main root. During the acclimatization period, seedlings were evaluated for survival, stem length, viable seedlings and rosette formation. Considering the conditions under which the experiment was conducted, it was concluded that MS culture medium with the addition of 1 mg L-1 of BAP allowed higher germination percentage and viable ‘BRS Kampai’ peach seedlings.

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Effect of Developmental Stage and Medium on Embryo Culture of Low Chill Peach Hybrids

Cited by 11 publications

References 6 publications

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

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