Effect of dielectric spacer thickness on signal intensity of surface plasmon field-enhanced fluorescence spectroscopy

Controlling on the luminescence property of RE ionic and molecular optical centers with nanostructure system

“…Chhabra等人 [96] 利用 DNA隔离层精确控制荧光分子(Cy3和Cy5)与金纳米 颗粒之间间距, 发现不同粒径的金纳米颗粒具有不 同的等离激元共振能量转移效率; 在所研究间距范 围内, 能量转移效率随着荧光分子与金纳米颗粒间 距的减小而增大. Murakami等人 [100] 认为荧光分子与 金属衬底之间的无辐射能量转移是荧光辐射效率降 图 5 荧光辐射速率随纳米颗粒与探针分子之间距离变化关系 [93] Figure 5 Fluorescence rate as a function of particle-surface distance z for a vertically oriented molecule [93] 低的主要原因. 他们采用聚苯乙烯分子作为隔离层 抑制无辐射能量转移, 发现合适厚度的聚苯乙烯介 质层有助于大幅度提高荧光辐射强度.…”

Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing

“…When this type of SPFS apparatus is used, an SPR sensory graph is first prepared by plotting the intensity of the reflected light against the incident light angle. Next, the incident angle of the light beam is carefully adjusted to a value 0.5°lower than the SPR angle [11] for each sample, and then the fluorescent intensity is determined at that angle. In a clinical setting, however, this is a time-consuming and tedious step.…”

“…SPFS uses a field-enhanced optical field of a surface plasmon mode for the excitation of fluorophores placed near the metaldielectric interface. The evanescent field of a surface plasmon is enhanced by a factor of approximately 15 compared with the incident field for a gold-water interface at the specific resonance angle, and then the signal decays exponentially into the dielectric medium, approximately Lz = 190 nm [11]. Thus, an SPFS-based sensor allows the sensitive determination of low concentrations of target molecules without the removal of unbounded complexes or B/F separation steps.…”

“…and then the signal decays exponentially into the dielectric medium, approximately Lz = 190 nm [11]. Thus, an SPFS-based sensor allows the sensitive determination of low concentrations of target molecules without the removal of unbounded complexes or B/F separation steps.…”

Surface modification of plasmonic nanostructured materials with thiolated oligonucleotides in 10 seconds using selective microwave heating

“…This is mostly due to the ability of plasmonic (surface plasmon-supporting) nanostructured materials to manipulate and transport electromagnetic energy, which afford for the realization of more efficient biological and electronic processes. In this regard, Surface Plasmon Resonance (SPR) [1–4], Surface Enhanced Raman Scattering (SERS) [5–7] and Surface Plasmon Fluorescence Spectroscopy (SPFS) [8–10] are the most commonly utilized plasmonics technologies today. The interest in plasmonic nanostructured materials is also due to their size (in the order of biomolecules themselves) and their amenability to the attachment of biological materials.…”