Effect of dietary selenium deficiency on the in vitro fertilizing ability of mice spermatozoa

Sánchez-Gutiérrez, Manuel; García-Montalvo, Eliud A.; Izquierdo-Vega, Jeannett A.; Razo, Luz M. Del

doi:10.1007/978-1-4020-9056-1_5

Cited by 14 publications

(16 citation statements)

References 35 publications

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“…Selenium (Se), an essential trace element for mammals, is an integral part of antioxidant systems [5]. Se-dependent glutathione peroxidase (GPx) has an important role in free radical protective mechanisms.…”

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confidence: 99%

Selenium and Vitamin E Improve the <i>In Vitro</i> Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes

et al. 2012

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Abstract. Selenium (Se) and vitamin E (Vit-E), as integral parts of antioxidant systems, play important roles for sperm and embryos in vitro. In this study, the effects of Se and Vit-E on the maturation, in vitro fertilization and culture to blastocysts of porcine oocytes and accumulation of ammonia in the culture medium during different development stages were investigated. The maturation was performed in modified tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, the fertilization medium was modified Tyrode's albumin lactate pyruvate (mTALP), and the embryo culture medium was modified North Carolina State University (mNCSU)-23. Se in the form of sodium selenite (SS) and seleon-L-methionine (SeMet) and Vit-E at different concentrations were also used. The incorporation and oxidation of 14 C(U)-glucose were assessed with a liquid scintillation counter. In this study, SeMet and SeMet+Vit-E increased oocyte maturation, fertilization and incorporation and oxidation of 14 C(U)-glucose significantly (P<0.05) compared with the control and other treatments. In addition, embryo development, specifically in terms of the numbers of morulae and blastocysts, significantly increased (P<0.05) with SeMet and SeMet+Vit-E. In contrast, the accumulation of ammonia was reduced with SeMet and SeMet+Vit-E compared with other treatments. These findings indicate that SeMet and SeMet+Vit-E may play important roles in reducing the accumulation of ammonia and subsequently in increasing the rate of maturation of porcine oocytes and fertilization, as well as development of the blastocyst and utilization of glucose in in vitro maturation, fertilization and culture to blastocysts of porcine oocytes.

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confidence: 99%

Selenium and Vitamin E Improve the <i>In Vitro</i> Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes

et al. 2012

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“…Previous studies have shown that even when the Se status of other tissues is low, the testes maintain Se content at original concentrations [8]. These findings, combined with the importance of Se in preventing sperm morphological abnormalities [2][3][4], demonstrate that Se is essential for the proper functioning of the male reproductive system. In this study, we compared the effects of supplementing either inorganic (sodium selenite) or organic (yeast-derived) Se on testicular gene expression patterns in roosters.…”

Section: Discussionmentioning

confidence: 67%

“…Previous studies have identified a strong relationship between Se status and sperm quality [4,[17][18][19][20][21][22]. Selenium localizes to the midpiece of sperm [23] and deficiency, depending on the severity, results in decrease motility, abnormal morphology, and infertility.…”

Section: Discussionmentioning

confidence: 98%

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Source of Selenium Supplementation Influences Testis Selenium Content and Gene Expression Profiles in Single Comb White Leghorn Roosters

Brennan

Pierce

Cantor

et al. 2011

Biol Trace Elem Res

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Spermatogenesis is a tightly regulated, selenium-dependent process. Nutritional deficiencies, including Se, have been associated with decreased fertility. During Se depletion, testes preferentially retain Se while other tissues are depleted. This study was aimed at evaluating the effect of Se source (inorganic or organic yeast derived) on testes weight, Se content, and gene expression. At 17 weeks of age, roosters were randomly assigned to one of three treatments: basal diet (control), basal diet + 0.3 mg organic Se/kg organic yeast-derived Se (YS; Sel-Plex®, Alltech Inc.), or basal diet + 0.3 mg inorganic Se /kg inorganic Se as sodium selenite (SS). At 40 weeks of age, seven roosters from each treatment were euthanized and testes removed. Testes weight did not differ between treatments, but Se content was greater (P ≤ 0.01) in YS than SS and control. Testicular differential gene expression profiling was accomplished using the Affymetrix Genechip® chicken genome array. Ingenuity® pathway analysis revealed that Se supplementation, regardless of source, results in the up-regulation of genes governing cell structure/morphology. The enrichment of such pathways was greater with YS than SS. These expression patterns suggest that aside from playing a role in antioxidant defense, Se, especially in the organic YS form, is useful for maintaining testicular cell structure.

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“…In recent years, the involvement of free radicals and oxidative damage has been proposed as an alternate mechanism for ammonia toxicity [4]. Selenium (Se), an essential trace element for mammals, is an integral part of antioxidant systems [5]. Se-dependent glutathione peroxidase (GPx) has an important role in free radical protective mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Effect of selenium and vitamin E on acrosome reaction in porcine spermatozoa

Tareq

Akter

Takagi

et al. 2009

Reprod Medicine & Biology

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Purpose Selenium (Se) and vitamin E (Vit-E), as an integral part of antioxidant systems, play an important role in the motility and acrosome reaction (AR) of mammalian spermatozoa. The purpose of this study was to investigate the effect of Se and Vit-E on motility, viability, AR and accumulation of ammonia in the culture medium during different incubation periods in porcine sperm. Methods Sperm samples were washed, swum-up and incubated at 37°C for 1 and 3 h in Sp-TALP medium supplemented with sodium selenite (SS), seleon L-methionine (SeMet) and Vit-E in the presence or absence of ammonia. Sperm motility was determined on the basis of movement quality examined by phase microscopy. Viability and AR of spermatozoa were assessed by Hoechst 33258 and chlortetracycline (CTC) staining technique, and accumulation of ammonia was measured by the indophenol method. The incorporation of 14 C(U)-glucose was assessed with a liquid scintillation counter. Results In experiment 1, the sperm motility, viability, AR and incorporation of 14 C(U)-glucose increased significantly (P \ 0.05) in SS, SeMet and Vit-E (5, 5 lg/l and 1.0 mM, respectively) compared with the control. In experiment 2, treatment of the sperm with SeMet and SeMet ? Vit-E in the presence of 300 lM ammonia also resulted in a significant increase (P \ 0.05) in the rate of motility, viability, AR and incorporation of 14 C(U)-glucose. In contrast, the accumulation of ammonia was reduced by SeMet and SeMet ? Vit-E compared with the other treatments. Conclusions These findings indicate that SeMet and SeMet ? Vit-E may play an important role in reducing the accumulation of ammonia and subsequently in increasing the rate of AR and the utilization of glucose in porcine spermatozoa.

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Effect of dietary selenium deficiency on the in vitro fertilizing ability of mice spermatozoa

Cited by 14 publications

References 35 publications

Selenium and Vitamin E Improve the <i>In Vitro</i> Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes

Selenium and Vitamin E Improve the <i>In Vitro</i> Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes

Source of Selenium Supplementation Influences Testis Selenium Content and Gene Expression Profiles in Single Comb White Leghorn Roosters

Effect of selenium and vitamin E on acrosome reaction in porcine spermatozoa

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