Purpose: This study assessed the adhesion of C-point filling system to retreated root canal walls. Materials and Methods: Eighty extracted upper incisors were prepared using Protaper Universal rotary files then the samples were distributed into 2 main groups. Group I: primary treated, Group II: retreated. Each group was further distributed into 4 subgroups; Subgroup A: Samples were filled with gutta-percha. Subgroup B: Samples were filled with gutta-percha and AH Plus sealer. Subgroup C: Samples were filled with C-point. Subgroup D: Samples were filled with C-point and BioCeramic sealer (BC). The push out test was carried out. The sealer penetration inside the dentinal tubules was evaluated using scanning electron microscope (SEM). Results: regarding the results of group I(primary treated) and II (retreated), at all levels, the highest mean bond strength value was recorded in primary treated samples, while the lowest value was recorded in retreated samples. Among the tested groups there was no statistically significant difference except for subgroup B(Gutta-percha/AH plus) at the middle and apical levels while in subgroup D(C-point/BC sealer) at the middle level, there was a statistical significant difference among the tested groups. SEM observation of sealer/ dentin interface revealed few traces of sealer penetration in samples retreated with C-point and BC sealer. Conclusion: C-Point/BC sealer showed better adhesion to the intraradicular dentin in primarily treated group compared to gutta-percha /AH Plus sealer. While the adhesion of C-point filling system to intraradicular dentin negatively affected by the retreatment procedure.