Effect of different commercial spawning agents and thermal regime on the effectiveness of pikeperch, Sander lucioperca (L.), reproduction under controlled conditions

Abstract

Pikeperch, Sander lucioperca (L.), has been identified as one of the most perspective candidates for diversification of freshwater aquaculture. However, some aspects of production are still being developed, and controlled reproduction is one of the bottlenecks. The aim of the present study was to compare the effectiveness of different commercial spawning agents in the induction of final oocyte maturation (FOM) and ovulation in wild spawners. Within the study, four spawning agents [human chorionic gonadotropin (hCG), mixed human and horse gonadotropin (PG-600), carp pituitary (CPH) and mammalian GnRH analogue combined with metoclopramide (Ovopel)] in different thermal regimes (13 and 15°C) were tested. In both thermal regimes, the highest (P \ 0.05) ovulation rate among the treatment groups was obtained after stimulation with hCG (100 % in both cases). Latency time was the shortest in groups where CPH was used (2-3 and 3-4 days for 15 and 13°C) and was similar in the remaining groups (3-4 and 4-5 days for 15 and 13°C, respectively). Embryo survival was the highest in groups treated with hCG (78.9 and 81.3 % at hatching stage for 15 and 13°C, respectively). Hormonal stimulation did not significantly affect spermiation rate or spermatozoa motility (P [ 0.05). Based on the obtained results, hCG can be recommended for induction of FOM and ovulation in pikeperch. In addition, the thermal regime within the tested range seemed to have no effect on the reproduction outcome, and the application of lower temperature only prolonged the time of ovulation.

Reproductive performance of indoor-reared pikeperch (Sander lucioperca) females after wintering in outdoor earthen ponds

“…Generally, these authors found decreased reproductive performance in virgin breeders, compared to older spawners. The indicators of reproductive success for wild breeders in the present study were low when compared to the results of previous studies (Zakęś & Demska‐Zakęś ; Żarski, Targońska, Kaszubowski, Kestemont, Fontaine, Krejszeff, Kupren & Kucharczyk ). This could be explained by the fact that the females used in this study were small and young, factors which are assumed to result in lower spawning success (Lappalainen, Dörner & Wysujack ; Kamler ).…”

Time of response to hormonal treatment but not the type of a spawning agent affects the reproductive effectiveness in domesticated pikeperch, Sander lucioperca

“…The results presented in this study did not confirm improved efficiency upon repeated sGnRHa administration, as has been reported for other freshwater non-salmonid teleosts (Kucharczyk et al, 2008). For pikeperch, repeated administration was previously tested with hCG (Zakęś et al, 2013), acetone-dried carp pituitary homogenate (Rónyai, 2007) or a mammalian GnRH analogue administered alone or together with dopamine antagonists (Křišt'an et al, 2013;Żarski et al, 2013). The results following application of those repeated injections had either negative or no effect (see Żarski et al, 2015).…”

“…However, huge intragroup variability in spawning effectiveness indicates an urgent need for additional studies that allow identification of the factors that condition egg quality, including 'time of response to the hormone' as a specific variable to be tested. Additionally, the testing of other spawning protocols, such as alternative thermal protocols, including lower and/or changeable temperature (since temperature is an important modulatory factor for FOM; Pourhosein Sarameh et al, 2012;Żarski et al, 2013) that allow control over oocyte maturation speed and, presumably, egg quality, is recommended for future studies.…”

Hormonal treatment affects sperm motility in the spined loach (Cobitis taenia, Pisces, Cobitidae)

“…On the other hand, natural gonadotropins (e.g. hCG) are particularly recommended to enhance reproduction in the Percidae family (Żarski, Horváth, Kotrik, Targońska, Palińska, Krejszeff, Bokor, Urbányi & Kucharczyk ; Żarski, Targońska, Kaszubowski, Kestemont, Fontaine, Krejszeff, Kupren & Kucharczyk ; Targońska, Szczerbowski, Żarski, Łuczyński, Szkudlarek, Gomułka & Kucharczyk ). It should be stressed that hCG has also been successfully applied in the reproduction of some other multiple–spawner fish including rudd ( Scardinius erythrophthalamus ) (Kucharczyk, Kujawa, Mamcarz & Wyszomirska ) and chub ( Leuciscus cephalus ) (Krejszeff, Targońska, Żarski & Kucharczyk ).…”