Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Abstract

The present article deals with the studies on the effect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF. Sodium dihydrogen phosphate (NaH(2)PO(4)) favored 34% higher enzyme yield than the control, which was followed by 19% higher activity in potassium dihydrogen phosphate (KH(2)PO(4)) in SSF at 0.015% w/v. The addition of Tween-20 in SmF showed a maximum yield of 12.6 U/mL while, SDS suppressed the growth of the fungus. None of the surfactants favored the enzyme yield in SSF. Calcium chloride (CaCl(2)) was extensively efficient in stimulating more than 55% higher phytase production in SmF at 0.01% v/v. In SSF, none of the metal salts stimulated phytase production.

Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains

“…With a further increase in the temperature to 30 o C, abundant growth was observed and a maximum phytase production was obtained at 96 h. In contrast, the activity of phytase decreased gradually as the temperature increased (Fig. 3), perhaps due to poor organism growth at higher temperatures and enzyme denaturation and temperaturedependent loss of activity (22). Sabu et al (23) and Gautam et al (19) reported phytase production at 30 o C using coconut oilcake (14.29 U/gds) and polystyrene (2.84 U/gds) with R. oligosporus.…”

“…In this study, the stimulatory effects of K 2 HPO 4 /Na 2 HPO 4 on phytase activity were moderated by the combined (WB:MOC) substrate. This may also have increased enzyme production to greater values than reported by Gunashree and Venkateswaran (22).…”

Phytase production by Bacillus subtilis US417 in submerged and solid state fermentations

“…The productivity of phytase by this bacterium in the case of SF [2.33 U/(g × h)] was higher than that of SSF [1.18 U/ (g × h)]. In fact, reports on the comparative production of phytase by these two fermentation processes are very scarce, with only one study available in the literature in which a direct comparison was undertaken, namely that by Gunashree and Venkateswaran (2008).…”

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

“…Fungi are highly exploited groups for phytase production due to their easy cultivation and extracellular production. A study on the optimization of media ingredients for phytase production by Aspergillus niger CFR 335 through submerged and solid-state fermentation was reported earlier (Gunashree and Venkateswaran 2008). In this work, an extracellular phytase was produced by Aspergillus niger CFR 335 through solid-state fermentation.…”