Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells

Sagnella, Anna; Pistone, Assunta; Bonetti, Simone; Donnadio, Anna; Saracino, Emanuela; Nocchetti, Morena; Dionigi, Chiara; Ruani, G.; Muccini, Michele; Posati, Tamara; Benfenati, Valentina; Zamboni, R.

doi:10.1039/c5ra20684g

Cited by 44 publications

(36 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…19.6a), recorded at room temperature, confirmed that the sample obtained by our procedure Fig. 19.6 Chemo-physical properties of in loco produced RSF and NSF solution and films [23] is pure and that the aminoacids composition is in accordance with previous literature report [23,24]. SF conformation influences the biodegradation, mechanical and optical properties of the SF films.…”

Section: Chemo-physical Properties Of Regenerated and Native Silk Fibsupporting

confidence: 83%

“…We have then demonstrated that the application of different fabrication methods to prepare SF films influences the chemo-physical properties of SF films and their interaction with neural cells. In particular, we found that hydrophobic surfaces induce proliferation of astrocytes and neuron adhesion, whereas hydrophilic surfaces promote a remarkable neurite outgrowth [24]. Interestingly, surface properties such as roughness and wettability, mechanical resistance, dissolution and degradation profiles can be affected by fabrication method.…”

Section: Biological Properties Of Silk Films Prepared By Different Mementioning

confidence: 91%

See 1 more Smart Citation

Silk Fibroin Based Technology for Industrial Biomanufacturing

Benfenati

Toffanin

Chieco

et al. 2019

Factories of the Future

Self Cite

View full text Add to dashboard Cite

Natural biomaterials are more and more used for the development of high technology solutions, setting the scene for a bio-based material economy that responds to the increasing demand of environmentally friendly products. Among natural biomaterials, silk fibre protein called silk fibroin (SF) produced by the Bombyx mori L. insect, recently found a broad range of applications in biomedical field. SF substrates display remarkable properties like controlled biodegradability, flexibility, mechanical resistance and optical transparency, solution processability. These properties combined with the water-based extraction and purification process make SF a promising material for sustainable manufacturing enabling to partially replace synthetic, plastic-based and non-biodegradable material use. The use of SF interfaces in biocompatible electronic or photonic devices for advanced biomedical applications has been recently highlighted. However, the use of a natural biomaterial is challenging due to the complex nature of the biological molecule, and it requires to tightly control biomaterial properties during all the manufacturing steps. In this work, we show the results obtained by in loco production of raw-material, defining the best condition for silkworm selection and growth. The assessment and standardization of extraction/purification methodology are reported with reference to the high purity and remarkable performance in terms of chemo-physical property and biocompatibility of the obtained SF products. Finally, we demonstrate the fabrication, characterization and validation of microfluidic and photonic components of a lab-on-a-chip device for biodiagnostic based on biomanufactured SF.

show abstract

Section: Chemo-physical Properties Of Regenerated and Native Silk Fibsupporting

confidence: 83%

Section: Biological Properties Of Silk Films Prepared By Different Mementioning

confidence: 91%

Silk Fibroin Based Technology for Industrial Biomanufacturing

Benfenati

Toffanin

Chieco

et al. 2019

Factories of the Future

Self Cite

View full text Add to dashboard Cite

show abstract

“…Silk fibroin films are typically prepared by casting the aqueous fibroin solution [51]. Other reported techniques include spin-coating [52], vertical deposition [53], and spin assisted layer-by-layer assembly [54]. Silk fibroin films can be easily obtained by casting SF solution on a smooth and clean plate with subsequent natural evaporation or drying under a certain temperature.…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

“…In their report, a glass coverslip was inserted vertically in the SF solution in an oven at 50 °C; and because of the lateral capillary force and surface tension drive between the fibroin solution and the glass coverslip, silk fibroin solution could be deposited on the glass coverslip and dried to obtain the transparent SF film. However, films prepared by this method showed a non-homogeneous texture, which was caused by the inherent feature of the vertical deposition method [53]. Jiang et al developed an ultrathin SF film (45 ± 5 nm) fabricated by the layer-by-layer assembly technique; the resulting film displayed high elastic modulus and ultimate tensile strength due to its gradual self-reinforcing structure [54].…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

View full text Add to dashboard Cite

The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

show abstract

“…Indeed, the water contact angle value decreases from ≈68.2° for the KerAzA sample to 51.0° for the KerAzAPD red sample. This hydrophilicity value can be related both to the hydrophilic nature of the PD and to the different surface morphology induced by the coating and it is particularly suitable for cells adhesion and growth as also indicated for other biocompatible materials …”

Section: Resultsmentioning

confidence: 99%

Mild and Effective Polymerization of Dopamine on Keratin Films for Innovative Photoactivable and Biocompatible Coated Materials

Posati

Ferroni

Aluigi

et al. 2018

Macro Materials & Eng

Self Cite

View full text Add to dashboard Cite

Mussel‐inspired polydopamine (PD) coating represents a promising route for constructing functional materials and finely tuning or completely changing their surface properties. In this work, a mild and effective method to realize the deposition of PD on keratin‐based films is reported. Reactive oxygen species (ROS), generated by keratin films doped with a photosensitizer, e.g., Azure A (AzA), upon UV–vis and vis‐near IR irradiation, are exploited to obtain the PD coating. Interestingly, the use of vis‐near IR irradiation leads to an increased production of ROS by AzA resulting in a greater PD deposition. Compared to uncoated keratin films, the PD‐keratin coated materials show a granular but homogeneous surface and an increased hydrophilicity, maintaining the ROS generation ability of AzA. This work demonstrates a useful method to effectively and rapidly functionalize with PD materials that are sensitive to temperature, pH and UV light, such as keratin. The proposed strategy allows obtaining new multifunctional biomaterials of potential interest in tissue engineering and drug delivery; in particular, the use of PD‐keratin films for near IR laser bonding of ophthalmic tissue, which is a representative case in wound healing purpose, is shown.

show abstract

Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells

Abstract: In this study, we investigated the influence of processing methods on the chemo-physical properties of silk fibroin (SF) film and on their interaction with neural cells.

Cited by 44 publications

References 43 publications

Silk Fibroin Based Technology for Industrial Biomanufacturing

Silk Fibroin Based Technology for Industrial Biomanufacturing

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Mild and Effective Polymerization of Dopamine on Keratin Films for Innovative Photoactivable and Biocompatible Coated Materials

Contact Info

Product

Resources

About