Effect of different floatation solutions on E. tenella oocyst purification and optimization of centrifugation conditions for improved recovery of oocysts and sporocysts

Qi, Nanshan; Liao, Shenquan; Abuzeid, Asmaa M.I.; Li, Juan; Wu, Caiyan; Lv, Minna; Lin, Xuhui; Hu, Junjing; Xiao, Wenwan; Sun, Mingfei; Li, Guoqing

doi:10.1016/j.exppara.2020.107965

Cited by 2 publications

(1 citation statement)

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“…E. acervulina oocysts were isolated from field samples of unvaccinated chicken feces on a collaborating farm.. Briefly, fresh samples were subjected to centrifugation at 8000× g for 5 min using 50% sodium hypochlorite (NaClO) floatation solution [ 26 ]. Isolated oocysts were then sporulated using 2.5% potassium dichromate (K 2 Cr 2 O 7 ).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from Allium cepa against Eimeria acervulina Sporozoites

Aguinaga¹,

Mut-Salud²,

Falcón-Piñeiro³

et al. 2022

Microorganisms

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Among the alternatives to control avian coccidiosis, alliaceous extracts stand out due to their functional properties. Despite this, most of the references are focused just on garlic. In this study, we analyze the in vitro effects of propyl-propane thiosulfinate (PTS) and propyl-propane thiosulfonate (PTSO), two organosulfur compounds from onion, on MDBK cells infected with sporozoites of Eimeria acervulina. To this aim, two different experiments were performed. In the first experiment, sporozoites were previously incubated for 1 h at 1, 5 and 10 µg/mL of PTS or PTSO and added to MDBK cells. In the second experiment, MDBK cells were first incubated for 24 h at different concentrations of PTS or PTSO and then infected with E. acervulina sporozoites. Then, 24 h after inoculation, the presence of E. acervulina was quantified by qPCR. MDBK viability was measured at 72 h post-infection. Sporozoites incubated at 10 µg/mL of PTS and PTSO inhibited the capability to penetrate the cells up to 75.2% ± 6.44 and 71.7% ± 6.03, respectively. The incubation of MDBK with each compound resulted in a preventive effect against sporozoite invasion at 1 µg/mL of PTS and 1 and 10 µg/mL of PTSO. Cells incubated with PTSO obtained similar viability percentages to uninfected cells. These results suggest that the use of PTS and PTSO is a promising alternative to coccidiosis treatment, although further in vivo studies need to be performed.

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Section: Methodsmentioning

confidence: 99%