Effect of Different Methods of Bone Preparation on the Skeleton of the African Giant Pouched Rat (Cricetomys gambianus)

Abstract

SUMMARY:This study was conducted using 12 Adult African Giant Pouched rats of both sexes to compare and to see the effect of three different methods of bone preparation on the bones of the African Giant Rat (Cricetomys gambianus). Five rats were used for maceration method, three for burial method and four rats for chemical method (involving two rats each for 3% and 5% solution of Sodium Hydroxide). Chemical preparation with Sodium Hydroxide was found to be the best method in terms of time required to complete the procedure, number of bones recovered, colour of the bones and odour of the preparation. However, the chemical method has the disadvantage of dissolving and cracking the bones if the concentration used is high and prompt attention is not given to the preparation.

Comparative morphological interpretations on the bones of the pelvic limb of New Zealand rabbit (Oryctolagus cuniculus) and domestic cat (Felis domestica)

“…The animal exsanguinations were carried out through the common carotid artery under complete anesthesia. After evisceration and dissection of pelvic limbs from the trunk and removal of the skin and muscles carefully, the bony preparations were performed according to Onwuama et al [9].…”

Osteometrical Study of Sacrum and Coccygeal Vertebrae in a Marsh Crocodile (Crocodylus palustris)

“…Sacrococcygeal region of the carcass was skinned and defleshed to the extent possible. After evisceration, the remaining carcass was buried at 4 feet depth in ground as per Onwuama et al, (2012). The macerated bones were removed after about eleven months.…”

A study of the progression of damage in an axially loaded Branta leucopsis femur using X-ray computed tomography and digital image correlation

“…The bones were then cleaned following methodology of Cauble (2010) and Nawrocki (1997) . This methodology entails taking care of the preparation of specimens such that the procedure does not weaken the bones before carrying out the mechanical test ( Boyle, 2010 ; Grygon, 2010 ; Onwuama et al, 2012 ). All bones (including those in the previous section) were first treated with 75% saline solution to avoid use of organic/chemical.…”