Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Lovio-Fragoso, José Pablo; Hayano-Kanashiro, Corina; López‐Elías, José Antonio

doi:10.3856/vol47-issue2-fulltext-17

Cited by 14 publications

(15 citation statements)

References 25 publications

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“…In the haptophyte Isochrysis galbana, chlorophyll content decreased; however, an increase in carotenoids was observed, suggesting that this may be a protection mechanism against photo-oxidative stress [102]. Recent studies in C. muelleri cultured in F media reported that the highest content of chlorophyll a was found at 72 µM Pi, while at 7 and 18 µM Pi (considered as low P concentrations in the study), the chlorophyll a content was significantly reduced [35].…”

Section: Effect Of P Limitation On the Photosynthetic Apparatus And Pigment Content In Diatomsmentioning

confidence: 61%

“…On the other hand, in studies carried out in Chaetoceros muelleri grown in F medium, P concentration was doubled compared to the control, and a higher content of dry biomass was found in the control treatment compared to the P excess treatment. However, no significant differences were found in cell density between the control and excess concentrations [35].…”

Section: P Effect On Growth and Biomass Productionmentioning

confidence: 66%

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Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

Lovio-Fragoso

Jesús-Campos

López‐Elías

et al. 2021

Biology

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Diatoms are the most abundant group of phytoplankton, and their success lies in their significant adaptation ability to stress conditions, such as nutrient limitation. Phosphorus (P) is a key nutrient involved in the transfer of energy and the synthesis of several cellular components. Molecular and biochemical mechanisms related to how diatoms cope with P deficiency are not clear, and research into this has been limited to a few species. Among the molecular responses that have been reported in diatoms cultured under P deficient conditions is the upregulation of genes encoding enzymes related to the transport, assimilation, remobilization and recycling of this nutrient. Regarding biochemical responses, due to the reduction of the requirements for carbon structures for the synthesis of proteins and phospholipids, more CO2 is fixed than is consumed by the Calvin cycle. To deal with this excess, diatoms redirect the carbon flow toward the synthesis of storage compounds such as triacylglycerides and carbohydrates, which are excreted as extracellular polymeric substances. This review aimed to gather all current knowledge regarding the biochemical and molecular mechanisms of diatoms related to managing P deficiency in order to provide a wider insight into and understanding of their responses, as well as the metabolic pathways affected by the limitation of this nutrient.

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Section: Effect Of P Limitation On the Photosynthetic Apparatus And Pigment Content In Diatomsmentioning

confidence: 61%

Section: P Effect On Growth and Biomass Productionmentioning

confidence: 66%

Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

Lovio-Fragoso

Jesús-Campos

López‐Elías

et al. 2021

Biology

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“…A study on S. marinoi (Bertozzini et al 2013) showed that the reduction in nitrate concentration remarkably induced a decrease in biomass production. Likewise, in Chaetoceros muelleri, growth rates and cell densities increased as the phosphate (P) concentration increased from 7 lM to 144 lM (Lovio-Fragoso et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, in Chaetoceros muelleri , growth rates and cell densities increased as the phosphate (P) concentration increased from 7 μM to 144 μM (Lovio‐Fragoso et al. 2019).…”

Section: Discussionmentioning

confidence: 99%

Responses of triacylglycerol synthesis in Skeletonema marinoi to nitrogen and phosphate starvations

Zhang

Xing

Wang

et al. 2020

Journal of Phycology

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Skeletonema marinoi is one of the most widespread marine planktonic diatoms in temperate coastal regions and sometimes can form massive blooms. Yet, the molecular mechanisms of triacylglycerol (TAG) synthesis in nutrient‐deficient conditions for this species are still unknown. This study aimed to investigate how the TAG biosynthetic pathway of S. marinoi reacts to the culture age and nitrogen (N) or phosphorus (P) deficiency at molecular levels. Meanwhile, we also described the physiological and biochemical changes of S. marinoi in response to N or P starvation over time. To obtain reliable qRT‐PCR data, six putative reference genes were identified for assessing expression stability using geNorm and BestKeeper software, and Actin exhibited the most stable expression across 45 tested S. marinoi samples. We found that the expression of TAG biosynthesis‐related genes and ACCase enzyme activity varied in response to the different nutrient conditions and culture age. Taken together, we speculated that the capacity of TAG biosynthesis in S. marinoi is induced by N or P stress, and increases with culture age. Furthermore, TAG biosynthesis appears to respond more strongly to P deficiency than to N deficiency. Our study provides important insights into how diatoms regulate the TAG biosynthetic pathway when stressed by nutrient limitation. Besides, the data obtained from this study also provide useful clues for further exploring genes that can be used for metabolic engineering to enhance lipid production.

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“…The accumulation of phycobiliproteins requires sufficient phosphorus because it is degraded under phosphorus starvation conditions [96]. Protein accumulation was not significantly affected under different phosphorus concentrations in Chaetoceros muelleri [97], which may be because phosphorus is not a main component of proteins. On the other hand, similar to nitrogen starvation, phosphorus limitation has been widely used to produce various high-value secondary metabolites and storage lipids and carbohydrates.…”

Section: Phosphorusmentioning

confidence: 91%

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

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Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

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Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Cited by 14 publications

References 25 publications

Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

Responses of triacylglycerol synthesis in Skeletonema marinoi to nitrogen and phosphate starvations

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

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