Effect of different polyphenol sources on the efficiency of ellagic acid release by Aspergillus niger

Abstract

Fungal hydrolysis of ellagitannins produces hexahydroxydiphenic acid, which is considered an intermediate molecule in ellagic acid release. Ellagic acid has important and desirable beneficial health properties. The aim of this work was to identify the effect of different sources of ellagitannins on the efficiency of ellagic acid release by Aspergillus niger. Three strains of A. niger (GH1, PSH and HT4) were assessed for ellagic acid release from different polyphenol sources: cranberry, creosote bush, and pomegranate used as substrate. Polyurethane foam was used as support for solid-state culture in column reactors. Ellagitannase activity was measured for each of the treatments. Ellagic acid was quantified by high performance liquid chromatography. When pomegranate polyphenols were used, a maximum value of ellagic acid (350.21 mg/g) was reached with A. niger HT4 in solid-state culture. The highest amount of ellagitannase (5176.81 U/l) was obtained at 8h of culture when cranberry polyphenols and strain A. niger PSH were used. Results demonstrated the effect of different polyphenol sources and A. niger strains on ellagic acid release. It was observed that the best source for releasing ellagic acid was pomegranate polyphenols and A. niger HT4 strain, which has the ability to degrade these compounds for obtaining a potent bioactive molecule such as ellagic acid.

Role of ellagic acid for the prevention and treatment of liver diseases

“…To state briefly, it is a dimeric derivative of gallic acid. EA has numerous health benefits with considerable focus on its pharmacological role and suggested as a rationale lead for drug development (Sepúlveda et al, 2016;Shakeri, Zirak, & F I G U R E 2 Promiscuous molecular targets regulated by EA. All the molecular targets are expanded in the abbreviation section Sahebkar, 2018).…”

A Green Technology for Control of Avocado Necrotic Fungi Using Bioactive Coatings

“…The obtained extract was decanted and filtered using a filter paper. The filtered solution was passed through Amberlite-XAD16 ® with the conditions described for polyphenols recovery [23] to obtain a powder rich in total polyphenols from L. tridentata (PTP).…”

Ellagic acid production by solid-state fermentation influenced by the inert solid supports

“…Recently, it was shown the inductive effect of a purified extract of pomegranate ellagitannins in the production of ellagitannase by A. niger under SSF, authors reported an increase in the EAH p (Ascacio-Valdés et al, 2014). The effect of differents sources of polyphenols (cranberry, creosote bush and pomegranate) and fungal strains (A. niger GH1, A. niger HT4 and A. niger PSH) on EA release have been evaluated (Sepúlveda et al, 2016). The best combination was A. niger HT4 on pomegranate peels polyphenols (PP), reaching 350.21 mg g -1 .…”