Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Abstract

Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straw volumes and thawing rates on the postthaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4 °C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120 ºC) and finally stored in liquid nitrogen (-196 ºC) tank. Thawing of cryopreserved semen process was performed at 30 ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using a ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30 ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

Fertilizing capacity of cryopreserved sperm of Chirostoma jordani (Woolman, 1894)

“…This answer may explain sperm resistance to type and concentration of extender solution and CPAs (Ciereszko et al, 2014;Nynca et al, 2019), since both affect sperm motility. Results obtained in present research show that sperm concentration per oocyte depends on cryopreservation protocol, fertilization, species and even, among individuals of the same species, and they coincide with those obtained by other studies (Bozkurt & Yavaş, 2017;Ciereszko et al, 2014;Di lorio et al, 2019;Du et al, 2018;Kommisrud et al, 2020).…”

“…Cryopreservation had an effect over fertilizing capacity of semen, because their viability and motility decreased during thaw and post-thaw process, considerably. Because of this, it has been registered it is necessary a greater volume or concentration of semen to obtain percentages similar to those with fresh semen (Bozkurt & Yavaş, 2017;Ciereszko et al, 2014;Di lorio et al, 2019;Du et al, 2018;Kommisrud et al, 2020).…”

The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

“…Under these conditions, Linearity (LIN, the ratio of net distance moved to total path distance (VSL/VCL)) may become a very important determining parameter in finding the curvature of the route. Fertilization may be based on both the number of motile sperm cells, and their velocity (Rurangwa et al, 2004;Bozkurt and Yavaş, 2017).…”

Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

“…After stored in 30 days, thawing process was conducted. Put the mini straw into the water bath with temperature of 30 o C for 30 seconds (7). Then dripped the spermatozoa from mini straw on glass object then examined the motility, viability, abnormality, fertilization and hatching rates.…”

“…The extender is important for dilution of fish spermatozoa prior to cryopreservation. The most important function of the extender is to keep the spermatozoa in an immotile state until it is used (7). The diluents are required in cryopreservation to inhibit the energy used by spermatozoa and prolong the life of spermatozoa by reducing their activity (8).…”