The aim of this study was to investigate the effect of chlorhexidine on shear bond strength of self-etch adhesives to dentin. The crowns of 60 sound human premolars were horizontally sectioned to expose the coronal dentin. Dentin surfaces were polished with 320 grit silicon carbide papers, and were randomly divided into 4 groups (n = 15). In group 1, two-step self-etch adhesive resin (Clearfil SE Bond) was used. In group 2, the surface was rinsed with chlorhexidine 2% solution prior to Clearfil SE bond application. In groups 3 and 4, the procedure was repeated similar to groups 1 and 2. In this case, onestep self-etch adhesive resin (Clearfil S3 Bond) was used. Then Z100 composite resin was applied. Subsequent to thermocycling, the shear bond strengths were measured. Data were analyzed using oneway ANOVA. In this study, p < 0.05 was considered significant. There were no significant differences between groups (p = 0.321). The use of 2% chlorhexidine had no effect on the shear bond strength of two-step and one-step self-etching adhesive resins. Moreover, there was no significant difference in the shear bond strength values between two-step and one-step self-etching adhesive resins.