Effect of drying on conidial viability of<i>Penicillium frequentans</i>, a biological control agent against peach brown rot disease caused by<i>Monilinia</i>spp

Guijarro, Belén; Larena, Inmaculada; Melgarejo, P.; Cal, A. De

doi:10.1080/09583150500335897

Cited by 48 publications

(44 citation statements)

References 23 publications

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“…Biocontrol agents, such as Pantoea agglomerans (Costa et al 2002), Epicoccum nigrum (Larena et al 2003a), Penicillium oxalicum (Larena et al 2003b), P. frequentans (Guijarro et al 2006) and Candida sake (Abadias et al 2005), are heat sensitive and spraydrying of them adversely affects their viability. However, spray-drying is suitable for probiotic bacteria (Golowczyc et al 2010) and for Bacillus thuringiensis (Prabakaran and Hoti 2008).…”

Section: Introductionmentioning

confidence: 99%

Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8

et al. 2011

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The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.

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Section: Introductionmentioning

confidence: 99%

Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8

et al. 2011

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“…Diversas investigaciones han realizado estudios comparativos de tres métodos más comunes para la microencapsulación de probióticos; liofilización, secado por aspersión y pulverización en lecho fluidizado ( Guijarro et al, 2006;Larena et al, 2003;). El secado por congelamiento o también denominada liofilización es una de las técnicas más importantes, convenientes y exitosas para la preservación a largo plazo de microorganismos probióticos (Chávez y Ledeboer, 2007;Strasser et al, 2009), sin embargo resulta ser muy costoso y laborioso para ser desarrollado a escala industrial (Augustin y Hemar, 2009); el secado por atomización en contraste al liofilizado permite procesar grandes volúmenes de solución, su principal desventaja son sus altas temperaturas de proceso que pueden afectar drásticamente a la supervivencia de los microorganismos y por ende reducir significativamente la eficiencia (Silva et al, 2014).…”

Section: Introductionunclassified

Evaluación de la Estabilidad y Viabilidad de Dos Cepas Probióticas Microencapsuladas por Lecho Fluidizado

Cayra¹,

Dávila²,

Villalta³

et al. 2017

Inf. tecnol.

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“…Fluidized bed-drying is a costeffective method of drying that can be used to dry heat-sensitive microorganisms because the drying temperatures are relatively low. Fungi such as E. nigrum (Larena et al, 2003) and P. frequentans (Guijarro et al, 2006), the yeast-like fungus, A. pullulans (Mounir et al, 2007), and the yeast, C. sake CPA-2 (Usall et al, 2009) have all been successfully dried using fluidized bed-drying. In contrast, liquid formulations are the simplest way to stabilize the viability of microbial cells.…”

Section: Formulationmentioning

confidence: 99%

The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

293

149

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A B S T R A C TPostharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens.

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Effect of drying on conidial viability ofPenicillium frequentans, a biological control agent against peach brown rot disease caused byMoniliniaspp

Cited by 48 publications

References 23 publications

Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8

Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8

Evaluación de la Estabilidad y Viabilidad de Dos Cepas Probióticas Microencapsuladas por Lecho Fluidizado

The science, development, and commercialization of postharvest biocontrol products

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