Effect of eccentric and concentric contraction mode on myogenic regulatory factors expression in human vastus lateralis muscle

Abstract

Background: Skeletal muscle contractions are caused to release myokines by muscle ber. This study investigated the myogenic regulatory factors, as MHC I, IIA, IIX, Myo-D, MRF4, Murf, Atrogin-1, Decorin, Myonection, and IL-15 mRNA expression in the response of eccentric vs. concentric contraction.Methods: Eighteen healthy men were randomly divided into two eccentric and concentric groups, each of 9 persons. Isokinetic contraction protocols included maximal single-leg eccentric or concentric knee extension tasks at 60°/s with the dominant leg. Contractions consisted of a maximum of 12 sets of 10 reps, and the rest time between each set was 30 seconds. The baseline biopsy was performed four weeks before the study, and post-test biopsies were taken immediately after exercise protocols from Vastus Lateralis muscle. The gene expression levels evaluated using Real-Time PCR methods.Results: A signi cant difference in MyoD, MRF4, Myonection, and Decorin mRNA, were observed following eccentric or concentric contractions (P≤0.05). The MHC I, MHC IIA, IL-15 mRNA has been changed signi cantly compared to the pre-exercise in the concentric group (P≤0.05). While only MHC IIX and Atrogin-1 mRNA changed signi cantly in the eccentric group (P≤0.05). Additionally, the results showed a signi cant difference in MyoD, MRF4, IL-15, and Decorin were observed at the follow-up values between eccentric or concentric groups (P≤0.05). Conclusion: Our ndings highlight the growing importance of elucidating the different responses of muscle growth factors associated with a myogenic activity such as MHC IIA, Decorin, IL-15, Myonectin, Decorin, MuRF1, and MHC IIX mRNA in following to various types of exercise.