Effect of Ecklonia Cava Polyphenol Extract in House Ear Institute-Organ of Corti 1 Cells Against Cisplatin Ototoxicity: A Preliminary Study

Abstract

Objective: Cisplatin is a widely used agent for the treatment of adult and childhood malignancies. Side effects such as nephrotoxicity, neurotoxicity, and ototoxicity lead to dose limitations. Ecklonia cava polyphenol extract (ECP) is a molecule obtained from algae that live in seawater in the Far East. ECP has recently been shown to have protective effects against oxidative stress. The aim of this study was to evaluate the possible protective effects of ECP on cisplatin ototoxicity. Methods:In this study, we investigated the protective effects of ECP against cisplatin-induced cell death in mouse-derived House Ear Institute Organ of Corti (HEI-OC1) cochlear cells. Cisplatin (100 µM) and 1, 10, and 25 µM doses of ECP were administered to the cells, and the protective effects of ECP at 24 and 72 hours were investigated. Cell viability was evaluated by the WST-1 (water soluble tetrazolium salt).Results: Cisplatin (100 µM) reduced cell viability in both the 24 th and 72 nd hour evaluation. Although the 25 µM dose of ECP showed otoprotective effects in the 24 th hour, in the 72 nd hour this effect disappeared. Other doses of ECP showed no otoprotective effects in the 24 th and 72 nd hours. Conclusion:Although ECP showed some protective effects in the 24 th hour against cisplatin ototoxicity, these effects disappeared by the 72 nd hour. Further studies using recurrent and higher doses of ECP are required.