Effect of edge activators on the formation and transfection efficiency of ultradeformable liposomes

Lee, Eun Hye; Kim, Adele; Oh, Yu‐Kyoung; Kim, Chong‐Kook

doi:10.1016/j.biomaterials.2004.02.020

Cited by 123 publications

(58 citation statements)

References 21 publications

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“…The mean zeta potential of liposome solution including 3% (v/v) Tween 80 was −7.87 and −7.93 mV for S 75 PC and S 100 PC, respectively, whereas −6.63 mV was reported for the liposome prepared with a neutral lipid and Tween 80 (Lee et al 2005). The reason for the lower zeta potential in this study could be due to the acidic medium used for the hydration process.…”

Section: Effect Of Tween 80 In Hydration Mediummentioning

confidence: 52%

Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability

et al. 2007

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Despite its strong antitumor activity, paclitaxel (Taxol R ) has limited clinical applications due to its low aqueous solubility and hypersensitivity caused by Cremophor R EL and ethanol which is the vehicle used in the current commercial product. In an attempt to develop a pharmaceutically acceptable formulation that could replace Taxol R , a paclitaxel incorporated liposome has been constructed to improve solubility and physicochemical stability. The effect of various components of the liposome, including cholesterol and lipid, on the solubility and entrapment efficiency (EE) of paclitaxel was systematically investigated. The results showed that 5% (v/v) of polyethylene glycol 400 in the hydration medium of liposome significantly increased the solubility (up to 3.39 mg/mL) as well as the EE and the paclitaxel content in the liposome formulation composed of 10% (w/v) of S 100 PC with cholesterol (cholesterolto-lipid molar ratio = 10:90). When sucrose (sugar-to-lipid molar ratio = 2.3) was added as a lyoprotectant during the freeze-drying of the liposome, physicochemical stability of liposome was significantly improved. Moreover, the cytotoxicity of the final liposome formulation against MDA-MB-231 human breast cancer cell line was not significantly different from that of Taxol R . The enhanced aqueous solubility as well as the physicochemical stability of paclitaxel in the liposome formulation developed in this study could be a safer and effective alternative to the Cremophor R EL and ethanol formulation.

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Section: Effect Of Tween 80 In Hydration Mediummentioning

confidence: 52%

Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability

et al. 2007

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“…SC and TW80 are pharmaceutically acceptable and extensively investigated as edge activators for UDLs. 32,[39][40][41] Amphiphilic molecules such as bile salts (SC and sodium deoxycholate), Tweens (ethoxylated sorbitan esters), and Spans (sorbitan esters) have been extensively studied to provide deformability to liposomes. 14,32,41 Bile salts and Tweens were preferred over Spans since hydrophilic surfactants induce more deformability by increasing membrane fluidity and forming transient holes in lipid bilayers than lipophilic ones.…”

Section: Results and Discussion Preparation Of Mtx-udlsmentioning

confidence: 99%

“…Even though TW80 is a nonionic surfactant, UDLs with TW80 showed the negative value of zeta potential due to the partial hydrolysis of polyethylene oxide head groups (CH 2 -CH 2 -O) n of TW80. 40,44,46 TEM image of the optimized MTX-UDLs (MTX-UDLs-T3) confirmed their vesicular morphology with spherical shape and unilamellar structure ( Figure 2B). The initial large multilamellar vesicles were converted into unilamellar MTX-UDLs after extrusion process.…”

mentioning

confidence: 77%

Improved skin permeation of methotrexate via nanosized ultradeformable liposomes

Zeb¹,

Qureshi²,

Kim³

et al. 2016

IJN

131

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The aim of this study is to investigate methotrexate-entrapped ultradeformable liposomes (MTX-UDLs) for potential transdermal application. MTX-UDLs were prepared by extrusion method with phosphatidylcholine as a bilayer matrix and sodium cholate or Tween 80 as an edge activator. The physicochemical properties of MTX-UDLs were determined in terms of particle size, polydispersity index, zeta potential, and entrapment efficiency. The deformability of MTX-UDLs was compared with that of methotrexate-entrapped conventional liposomes (MTX-CLs) using a steel pressure filter device. The skin permeation of MTX-UDLs was investigated using Franz diffusion cell, and the skin penetration depth of rhodamine 6G-entrapped UDLs was determined by confocal laser scanning microscopy. MTX-UDLs showed a narrow size distribution, with the particle size of ~100 nm. The deformability of MTX-UDLs was two to five times greater than that of MTX-CLs. The skin permeation of MTX-UDLs was significantly improved compared with MTX-CLs and free MTX solution. The optimized UDLs (phosphatidylcholine: Tween 80 =7:3, w/w) showed a higher fluorescence intensity than conventional liposomes at every increment of skin depth. Thus, the optimized UDLs could be promising nanocarriers for systemic delivery of MTX across skin.

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“…In another study, ultradeformable liposomes containing edge activators sodium cholate or sodium deoxycholate could also deliver DNA into mice transdermally [177].…”

Section: Gene Delivery and Plasmid-based Vaccinesmentioning

confidence: 99%

(Phospho)lipid-based Nanosystems for Skin Administration

Vanić¹,

Holæter²,

Škalko‐Basnet

2015

CPD

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Nanotechnology and nanomedicine provide a platform for advanced therapeutic strategies for dermal and transdermal drug delivery. The focus of this review is on the current state-of-art in lipid-based nanotechnology and nanomedicine for (trans)dermal drug therapy. Drug delivery nanosystems based on the (phospho)lipid constituents are characterized and compared, with the emphasis on their ability to assure the controlled drug release to the skin and skin appendages, drug targeting and safety. Different types of liposomes, biphasic vesicles, particulate lipid-based nanosystems and micro-and nano-emulsions are discussed in more details. Extensive research in preclinical studies has shown that numerous parameters including the composition, size, surface properties and their combinations affect the deposition and/or penetration of carrier-associated drug into/through the skin, and consequently determine the therapeutic effect. The superiority of the most promising nanopharmaceuticals has been confirmed in clinical studies. We have selected several common skin disorders and provided overview over promises of nanodermatology in antimicrobial skin therapy, anti-acne treatment, skin oncology, gene delivery and vaccines. We addressed the potential toxicity and irritation issues and provided an overview of registered lipid-based products

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Effect of edge activators on the formation and transfection efficiency of ultradeformable liposomes

Cited by 123 publications

References 21 publications

Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability

Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability

Improved skin permeation of methotrexate via nanosized ultradeformable liposomes

(Phospho)lipid-based Nanosystems for Skin Administration

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