Effect of equal-channel angular pressing on structure and properties of Fe-Mn-С alloys for biomedical applications

Rybalchenko, O. V.; Anisimova, N. Yu.; Kiselevsky, M. V.; Rybalchenko, Georgy; Martynenko, Natalia; Бочвар, Н. Р.; Tabachkova, N. Yu.; Щетинин, И. В.; Konushkin, S. V.; Tokar, A. A.; Рааб, А. Г.; Добаткин, С. В.

doi:10.1016/j.mtcomm.2021.103048

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…Hemolysis and cytotoxicity were evaluated as described earlier in [32,33]. Evaluation of bioactive properties in vitro was carried out on samples in the form of a 1/8 disk.…”

Section: Methodsmentioning

confidence: 99%

Effect of High-Pressure Torsion on Microstructure, Mechanical and Operational Properties of Zn-1%Mg-0.1%Ca Alloy

Martynenko

Anisimova

Rybalchenko

et al. 2022

Metals

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A study of the effect of high-pressure torsion (HPT) on the structure, phase composition, corrosion resistance, mechanical properties and bioactivity in vitro of Zn-1%Mg-0.1%Ca alloy was performed. It was shown that HPT leads to refinement of the alloy microstructure with the formation of recrystallized α-Zn grains with an average size of 750 ± 30 nm, and grains of a mixture of different phases with a size of 38 ± 7 nm. In addition, precipitation of Ca-enriched particles ~20 nm in size was observed. X-ray phase analysis showed that the Zn-1%Mg-0.1%Ca alloy consists of five phases (Zn, Mg2Zn11, MgZn2, CaZn11 and CaZn13), whose volume fraction does not change after HPT. It was found that HPT does not lead to a deterioration in the corrosion resistance of the alloy. At the same time, HPT leads to an increase in the yield stress of the alloy from 135 ± 13 to 356 ± 15 MPa, the ultimate tensile strength from 154 ± 5 to 416 ± 31 MPa, and the ductility from 0.4 ± 0.1 to 5.5 ± 2.8%. No significant increase in hemolytic activity, bactericidal activity, and the ability to colonize the surface of the alloy by cells was revealed during the conducted studies. Additionally, there was no significant difference in these parameters in comparison with the control. However, HPT contributes to a decrease in the cytotoxicity of the alloy by an average of 10% compared to the annealed alloy. The conducted studies allow us to conclude that the Zn-1%Mg-0.1%Ca alloy is promising material for the development of biodegradable orthopedic medical implants.

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“…Hemolysis and cytotoxicity were evaluated as described earlier in [32,33]. Evaluation of bioactive properties in vitro was carried out on samples in the form of a 1/8 disk.…”

Section: Methodsmentioning

confidence: 99%

Effect of High-Pressure Torsion on Microstructure, Mechanical and Operational Properties of Zn-1%Mg-0.1%Ca Alloy

Martynenko

Anisimova

Rybalchenko

et al. 2022

Metals

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“…The intricate details of these techniques are reviewed in the referenced article [ 56 ]. It's worth highlighting the research on Fe–Mn–C alloys, which, after ECAP, exhibited the development of an ultrafine-grained structure [ 57 ]. This refinement enhanced the alloys' strength without compromising their biocompatibility.…”

Section: Strategy To Accelerate Fe Corrosionmentioning

confidence: 99%

Recent advances in Fe-based bioresorbable stents: Materials design and biosafety

Zhang,

Roux,

Rouchaud

et al. 2024

Bioactive Materials

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“…Сплавы Zn, Zn -0,8 %Cu, Zn -0,8 %Mn и Zn -0,8 %Li демонстрировали схожий результат [37]. Полученные нами данные об отсутствии негативного воздействия на клетки крови деформационной обработки методом РКУП, опосредующей изменение микроструктуры сплавов [38,39], согласуются с результатами наших предыдущих исследований, доказавших биосовместимость сплавов Zn -1 %Mg и Zn -1 %Mg -0,1 %Са, обработанных кручением под высоким давлением. Тем не менее, согласно работам других исследователей, можно сделать вывод о том, что применение иных методов обработки сплавов может ухудшить их биосовместимость.…”

Section: Fig 5 Micrographs Of Tumor Cells Of the Skov-3 Line On The S...unclassified

Study of biocompatibility and antitumor cytotoxic activity <i>in vitro</i> of Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys strengthened by equal angular pressing

Martynenko,

Anisimova,

Rybalchenko

et al. 2023

Rossijskij bioterapevtičeskij žurnal

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Introduction. The biological activity of potential biodegradable zinc-based alloys that are promising for oncoorthopedics was studied in this work. The alloys were processed by equal-channel angular pressing, which made it possible to increase their strength due to microstructure refinement and the ability to provide the functionality of osteosynthesis, fixed due to the metal structure developed on their basis.Aim. Investigation of effect of equal-channel angular pressing (ECAP) treatment on strength, ductility, degradation rate, biocompatibility in vitro and cytotoxicity against SKOV-3 tumor cells of the Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys.Materials and methods. The Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys in the initial state and after ECAP were used as objects of study, and blood cells of CBA mice were used as model systems. To assess the hemolytic activity, the samples were incubated with red blood cells for 4 and 24 hours at 37 °C, assessing the relative increase in the level of extracellular hemoglobin compared to the intact control. The cytotoxicity of the alloys was assessed by the change in the level of extracellular lactate dehydrogenase (LDH) activity after 24 hours of incubation with mononuclear white blood cells. The study of antitumor cytotoxic activity was carried out on human ovarian cancer cells of the SKOV-3 line in vitro, assessing their survival after 48 hours of incubation with alloy samples using the LDH test.Results. As a result of the studies, it was concluded that the studied alloys after ECAP treatment retained their biocompatibility, since there were no signs of hemolysis and cytotoxicity with respect to blood cells. However, contact with samples of all studied alloys in vitro induced a significant inhibition of the metabolic activity of the ovarian cancer cell culture in comparison with the control. Incubation with alloys samples leads to a decrease in cellular activity by an average of 49 % and 59 % for Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys, respectively. The addition of calcium to the composition of the alloy Zn – 1 %Mg contributed to the growth of antitumor cytotoxic activity.Conclusion. Thus, based on the results of assessing the hemolytic activity and cytotoxicity of the samples, we can conclude that the studied alloys are biocompatible. It was also found that Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys had a pronounced cytotoxic effect on SKOV-3 tumor cells. The obtained data indicate the prospects for the development of a new type of medical devices based on the studied alloys, promising, in particular, for oncoorthopedics: a metal structure developed on their basis can ensure the strength of osteosynthesis, reduce the risk of local recurrence of oncological disease and does not require a second operation to remove the device.

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Effect of equal-channel angular pressing on structure and properties of Fe-Mn-С alloys for biomedical applications

Cited by 13 publications

References 30 publications

Effect of High-Pressure Torsion on Microstructure, Mechanical and Operational Properties of Zn-1%Mg-0.1%Ca Alloy

Effect of High-Pressure Torsion on Microstructure, Mechanical and Operational Properties of Zn-1%Mg-0.1%Ca Alloy

Recent advances in Fe-based bioresorbable stents: Materials design and biosafety

Study of biocompatibility and antitumor cytotoxic activity <i>in vitro</i> of Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys strengthened by equal angular pressing

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