Effect of etanercept on the formation of epidural fibrosis in an experimental model

Kızılay, Zahir; Çetin, Nesibe Kahraman; Topçu, Abdullah; İsmailoğlu, Özgür; Ömürlü, İmran Kurt; Aktaş, Serdar; Yılmaz, Ali

doi:10.5137/1019-5149.jtn.18460-16.1

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Tumor necrosis factor-alpha (TNF-α) is an important cytokine which participates in many inflammation-related processes [5]. TNF-α receptors play a decisive role in inflammation and can be found on the cell membrane and in plasma in a soluble form.…”

Section: Introductionmentioning

confidence: 99%

Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model

Eken¹,

Ersoy²,

Kaygusuz³

et al. 2019

aoms

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Introduction Etanercept has been widely used in autoimmune diseases for blocking tumor necrosis factor α (TNF-α), which is an inflammatory cytokine. The anti-apoptotic and anti-inflammatory effects of etanercept against ischemia/reperfusion (I/R) injury have been shown for several tissues in rat studies, but to the best of our knowledge, there are no reports on its protective effects following similar injury in ovarian tissue. The aim of this study was to investigate whether etanercept has beneficial effects on ovarian I/R injury, as well as on ovarian reserve. Material and methods Twenty-four rats were randomly divided into four groups ( n = 6/group): sham (laparotomy only); sham + etanercept; I/R; and I/R + etanercept. Ischemia was induced for 3 h by twisting the ovary, and 24 h after detorsion the ovarian tissues were collected to evaluate histopathologic changes, glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and superoxide dismutase (SOD) concentrations for oxidative stress, 8-hydroxy-2′-deoxyguanosine (8-OHdG) for DNA damage, caspase-3 activity for apoptosis and ovarian follicle counts. To measure anti-Mullerian hormone (AMH), serum samples were drawn before and after surgery. Results Tissue GSH and SOD levels were significantly higher, while MDA and MPO levels were significantly lower in the I/R + etanercept group than in the I/R group ( p < 0.05, p < 0.01, respectively). Tissue 8-OHdG and caspase-3 activity were significantly lower in the I/R+etanercept group than in the I/R group ( p < 0.05, p < 0.01, respectively). Preoperative and postoperative AMH levels were compared and there was a significant reduction in the I/R and I/R + etanercept groups ( p < 0.001, p < 0.001). The reduction of AMH in the I/R + etanercept group was significantly lower than in the I/R group. The primordial, preantral and small antral follicle numbers were also significantly higher in the I/R + etanercept group compared to the I/R group ( p < 0.001 , p < 0.001, p < 0.005, respectively). Conclusions Etanercept attenuated inflammation and related oxidative stress and also helped to preserve ovarian reserve following ovarian I/R damage.

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Section: Introductionmentioning

confidence: 99%

Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model

Eken¹,

Ersoy²,

Kaygusuz³

et al. 2019

aoms

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“…Among the studies regarding the inflammatory response phase, the effect of specific inhibition of inflammatory cytokines and the use of anti-inflammatory agents on epidural fibrosis have been frequently examined in the literature. For the inhibition of specific inflammatory cytokines, etanercept, a TNF-α blocker, [1] tamoxifen, a TGF-β blocker, [7] methyl palmitate, [18] and dexmedetomidine [4] have been extensively investigated. Also, there are studies using diclofenac sodium [20] and curcumin [21] as anti-inflammatory agents.…”

Section: Discussionmentioning

confidence: 99%

“…Laminectomy membrane is formed in the area of bone defect due to bleeding in the deep layer of the paravertebral muscles after laminectomy. [1,2] Epidural fibrosis develops as a result of the exaggerated healing tissue in this area, causing adhesions in the dura mater and nerve roots, and is one of the most important causes of failed back surgery syndrome caused by recurrent pain after surgery. [3,4] Revision surgeries performed due to recurrent pain after surgery may cause complications such as epidural bleeding, nerve root damage and dural lacerations, and worsen the clinical outcome.…”

mentioning

confidence: 99%

Impact of radial extracorporeal shock wave therapy in post-laminectomy epidural fibrosis in a rat model

et al. 2021

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Objectives This study aims to investigate the effect of radial extracorporeal shock wave therapy (rESWT) treatment in the prevention of epidural fibrosis after laminectomy in rats. Materials and methods Eighteen 16-month-old male Sprague-Dawley rats weighing 300 g were used in this experimental study between November 2019 and February 2020. The rats were randomly divided into two groups as the control group (L3-L4 total laminectomy without any treatment) and the study group (L3-L4 total laminectomy plus rESWT). The rats were sacrificed at the postoperative sixth week and the lumbar spine was excised en bloc, fixed, and decalcified. Sections were stained with hematoxylin-eosin to evaluate epidural fibrosis, acute inflammation, chronic inflammation, and vascular proliferation. Results The median value and standard deviations were obtained based on histological examinations. Accordingly, epidural fibrosis decreased significantly in the study group compared to the control group. There was no statistically significant difference between the groups in terms of acute and chronic inflammation response and vascular proliferation. Conclusion The rESWT application immediately after surgery is effective in preventing epidural fibrosis after laminectomy in rats.

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Effectiveness of the Biophysical Barriers to the Peridural Fibrosis in Rat Laminectomy Model

Akkurt

Bakar

Dinçel

et al. 2018

Journal of Investigative Surgery

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Effect of etanercept on the formation of epidural fibrosis in an experimental model

Abstract: ABSTRACTafter each surgical procedure in patients who develop these clinical conditions and so the success rate of treatment decreases after each surgical procedure (23).

Cited by 5 publications

References 26 publications

Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model

Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model

Impact of radial extracorporeal shock wave therapy in post-laminectomy epidural fibrosis in a rat model

Effectiveness of the Biophysical Barriers to the Peridural Fibrosis in Rat Laminectomy Model

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