Effect of external pH on cyanobacterial phycobiliproteins production and ammonium excretion

doi:10.7324/jabb.2015.3408

Cited by 7 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The medium pH also affected PBP synthesis; the maximum productivities of S. salina were enhanced by a more alkaline pH (≈9–9.5), except for PE. A pH of 7–9 is apparently optimum for PBP accumulation, depending on the species at stake [ 44 , 47 ]. For instance, Pagels et al (2020) [ 21 ] found a pH of 9 to be optimum (under 20 °C and [NaCl] = 10 g·L −1 ) to attain maximum total PBP productivities (4.14 ± 0.71 mg·L culture −1 ·d −1 ) in Cyanobium sp.…”

Section: Discussionmentioning

confidence: 99%

“…This can be explained by the different responses of S. salina to variations in environmental conditions, which lead to shifts in metabolism, and responses to acclimatization that can favor one compound over another in order to maintain cell homeostasis. Keithellakpam et al (2015) [ 47 ] stated that, for certain strains of Phormidium sp. and Nostoc sp.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Temperature, pH, and NaCl Concentration on Biomass and Bioactive Compound Production by Synechocystis salina

Assunção

Amaro

Tavares

et al. 2023

Life

View full text Add to dashboard Cite

Synechocystis salina is a cyanobacterium that has biotechnological potential thanks to its ability to synthesize several bioactive compounds of interest. Therefore, this study aimed to find optimal conditions, in terms of temperature (15–25 °C), pH (6.5–9.5), and NaCl concentration (10–40 g·L−1), using as objective functions the productivities of biomass, total carotenoids, total PBPs, phycocyanin (PC), allophycocyanin (APC), phycoerythrin (PE), and antioxidants (AOXs) capacity of Synechocystis salina (S. salina) strain LEGE 06155, based in factorial design resorting to Box-Behnken. The model predicted higher biomass productivities under a temperature of 25 °C, a pH of 7.5, and low NaCl concentrations (10 g·L−1). Maximum productivities in terms of bioactive compounds were attained at lower NaCl concentrations (10 g·L−1) (except for PE), with the best temperature and pH in terms of carotenoids and total and individual PBPs ranging from 23–25 °C to 7.5–9.5, respectively. PE was the only pigment for which the best productivity was reached at a lower temperature (15 °C) and pH (6.5) and a higher concentration of NaCl (≈25 g·L−1). AOX productivities, determined in both ethanolic and aqueous extracts, were positively influenced by lower temperatures (15–19 °C) and higher salinities (≈15–25 g·L−1). However, ethanolic AOXs were better recovered at a higher pH (pH ≈ 9.5), while aqueous AOXs were favored by a pH of 8. The model showed that biomass production can be enhanced by 175% (compared to non-optimized conditions), total carotenoids by 91%, PC by 13%, APC by 50%, PE by 130%, and total PBPs by 39%; for AOX productivities, only water extracts exhibited a (marginal) improvement of 1.4%. This study provided insightful information for the eventual upgrading of Synechocystis salina biomass in the biotechnological market.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Temperature, pH, and NaCl Concentration on Biomass and Bioactive Compound Production by Synechocystis salina

Assunção

Amaro

Tavares

et al. 2023

Life

View full text Add to dashboard Cite

show abstract

“…The total PBP production and each PBP individually were chiefly promoted by red light treatment (apart from control, p > 0.05). These pigments tend to absorb under a yellow-red range, and several studies point at the red light as a stimulator of biomass growth and increased production of PBPs (in particular PC) [ 11 , 40 , 55 , 56 ]. For instance, in Arthrospira platensis subjected to different light qualities using LED (red, white, green, and yellow), PC was preferentially stimulated under the red spectrum [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Light Modulation for Bioactive Pigment Production in Synechocystis salina

et al. 2022

View full text Add to dashboard Cite

Cyanobacteria are microorganisms that are well-adapted to sudden changes in their environment, namely to light conditions. This has allowed them to develop mechanisms for photoprotection, which encompass alteration in pigment composition. Therefore, light modulation appears to be a suitable strategy to enhance the synthesis of specific pigments (e.g., phycocyanin) with commercial interest, in addition to conveying a more fundamental perspective on the mechanisms of acclimatization of cyanobacterium species. In this study, Synechocystis salina was accordingly cultivated in two light phase stages: (i) white LED, and (ii) shift to distinct light treatments, including white, green, and red LEDs. The type of LED lighting was combined with two intensities (50 and 150 µmolphotons·m−2·s−1). The effects on biomass production, photosynthetic efficiency, chlorophyll a (chl a) content, total carotenoids (and profile thereof), and phycobiliproteins (including phycocyanin, allophycocyanin, and phycoerythrin) were assessed. White light (under high intensity) led to higher biomass production, growth, and productivity; this is consistent with higher photosynthetic efficiency. However, chl a underwent a deeper impact under green light (high intensity); total carotenoids were influenced by white light (high intensity); whilst red treatment had a higher effect upon total and individual phycobiliproteins. Enhanced PC productivities were found under modulation with red light (low intensities), and could be achieved 7 days earlier than in white LED (over 22 days); this finding is quite interesting from a sustainability and economic point of view. Light modulation accordingly appears to be a useful tool for supplementary studies pertaining to optimization of pigment production with biotechnological interest.

show abstract

“…Produzem como principal metabólito as ficobiliproteínas (FBP), em um complexo denominado ficobilissoma, responsável pela captação de luz na fotossíntese. As FBP são proteínas pigmentadas que possuem diversas aplicações como corantes naturais para alimentos e produtos cosméticos, marcadores fluorescentes moleculares, entre outros a depender de seu grau de pureza (KEITHELLAKPAM et al, 2015;.…”

Section: Cianobactériasunclassified

“…Pesquisas em biocompostos têm aumentado nas últimas décadas, trazendo consigo um avanço nos estudos em microrganismos como as cianobactérias, devido à possibilidade de aplicações comerciais em diferentes áreas, como indústrias alimentícias, sejam elas relacionadas à nutrição humana ou animal; indústrias químicas e farmacêuticas; ou ainda aplicados no tratamento de resíduos, e na produção de energia, entre outros (KEITHELLAKPAM et al, 2015;.…”

Section: Introductionunclassified

Produção de Ficocianina de Nostoc sp. e Anabena variabilis e recuperação por sistemas aquosos bifásicos

Teixeira¹

View full text Add to dashboard Cite

Aos meus pais, pelo apoio, amor incondicional, incentivo constante, confiança, exemplo e educação que me deram durante a vida. Aos meus irmãos Thais e Thiago, pelo incentivo e companheirismo. Ao Kaio, pela compreensão, carinho, companheirismo e apoio nos momentos difíceis.Agradeço também à minha orientadora Juliana, pela confiança em mim depositada, incentivo, pelos conhecimentos transmitidos, discussões e profissionalismo no desenvolvimento do trabalho.Ao Arthur, pelo apoio nas atividades e transmissão de conhecimentos os quais me auxiliaram durante a realização deste trabalho. Aos alunos de IC, Luis e Warlley pela companhia e apoio nas atividades.À minha família e amigos por estarem sempre ao meu lado e proporcionarem momentos alegres e divertidos.A todos os mestrandos, doutorandos, funcionários e professores do NUCBIO, pela companhia, ajuda e carinho durante todo este tempo de trabalho.

show abstract

Effect of external pH on cyanobacterial phycobiliproteins production and ammonium excretion

Cited by 7 publications

References 20 publications

Effects of Temperature, pH, and NaCl Concentration on Biomass and Bioactive Compound Production by Synechocystis salina

Effects of Temperature, pH, and NaCl Concentration on Biomass and Bioactive Compound Production by Synechocystis salina

Light Modulation for Bioactive Pigment Production in Synechocystis salina

Produção de Ficocianina de Nostoc sp. e Anabena variabilis e recuperação por sistemas aquosos bifásicos

Contact Info

Product

Resources

About