Effect of extraction condition on protein recovery and phenolic interference in Pacific dulse (Devaleraea mollis)

Mendez, Rufa; Kwon, Jung Yeon

doi:10.1007/s10811-021-02467-3

Cited by 17 publications

(10 citation statements)

References 55 publications

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“…The protein content of leaves was assessed in fresh leaves of maize using bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, US as a reference according to the described protocol of Mendez and Kwon [38]. Fresh leaves (0.1 g) were crushed in a mortar and pestle with 1 mL of phosphate buffer (pH 7.5) and centrifuged for 10 min at 3000 rpm.…”

Section: Protein Content Of Leavesmentioning

confidence: 99%

Bacillus mycoides PM35 Reinforces Photosynthetic Efficiency, Antioxidant Defense, Expression of Stress-Responsive Genes, and Ameliorates the Effects of Salinity Stress in Maize

Ali

Wang

Saleem

et al. 2022

Life

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Soil salinity is one of the abiotic constraints that imbalance nutrient acquisition, hampers plant growth, and leads to potential loss in agricultural productivity. Salt-tolerant plant growth-promoting rhizobacteria (PGPR) can alleviate the adverse impacts of salt stress by mediating molecular, biochemical, and physiological status. In the present study, the bacterium Bacillus mycoides PM35 showed resistance up to 3 M NaCl stress and exhibited plant growth-promoting features. Under salinity stress, the halo-tolerant bacterium B. mycoides PM35 showed significant plant growth-promoting traits, such as the production of indole acetic acid, siderophore, ACC deaminase, and exopolysaccharides. Inoculation of B. mycoides PM35 alleviated salt stress in plants and enhanced shoot and root length under salinity stress (0, 300, 600, and 900 mM). The B. mycoides PM35 alleviated salinity stress by enhancing the photosynthetic pigments, carotenoids, radical scavenging capacity, soluble sugars, and protein content in inoculated maize plants compared to non-inoculated plants. In addition, B. mycoides PM35 significantly boosted antioxidant activities, relative water content, flavonoid, phenolic content, and osmolytes while reducing electrolyte leakage, H2O2, and MDA in maize compared to control plants. Genes conferring abiotic stress tolerance (CzcD, sfp, and srfAA genes) were amplified in B. mycoides PM35. Moreover, all reactions are accompanied by the upregulation of stress-related genes (APX and SOD). Our study reveals that B. mycoides PM35 is capable of promoting plant growth and increasing agricultural productivity.

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Section: Protein Content Of Leavesmentioning

confidence: 99%

Bacillus mycoides PM35 Reinforces Photosynthetic Efficiency, Antioxidant Defense, Expression of Stress-Responsive Genes, and Ameliorates the Effects of Salinity Stress in Maize

Ali

Wang

Saleem

et al. 2022

Life

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“…In the same manner, a combination of sonication and OS has been proven to increase SP yield and extractability [24]. Nonetheless, one of the most valuable pretreatment methods involves the application of glucanases to the extraction mix to maximize SP yield [25]. Using fresh, dried or freezedried seaweed samples may also influence the resulting SP yield, although yield variations are not significant.…”

Section: Extraction Technologiesmentioning

confidence: 99%

“…In fact, proteins can be extracted by their solubilization at different pH values, involving the sequential use of differently buffered solutions. Nevertheless, proteins are generally co-extracted with other interferents, such as sugar or phenolic compounds [25,28], forcing the application of protein precipitation to achieve the isolation and purification of the extracted SP. Moreover, except for EAE, extraction methods promote an unspecific protein hydrolysis, which is also accompanied by the liberation of intracellular proteases from cell walls that may further degrade protein structures.…”

Section: Extraction Technologiesmentioning

confidence: 99%

“…The application of targeted polysaccharide-digesting enzymes, such as cellulases, hemicellulases, β-glucanases, and xylanases has been described as a food-grade approach to breakdown the macroalgae cell wall. Thus, commercial enzyme cocktails have been proven to be successful for this purpose [25]. However, seaweed cell wall composition can vary among phyla and species and, therefore, a right choice of carbohydrase(s), together with the optimization of operating conditions (enzyme:substrate ratio (E:S), temperature, pH) of individual enzymes or cocktails must be proved prior to large-scale extraction to maximize protein recovery.…”

Section: Enzyme-assisted Extractionmentioning

confidence: 99%

“…This resulted in a total protein recovery of 8.39% when compared to that obtained following OS and mechanical shear (6.77% and 6.92%, respectively). Therefore, EAE employing glucanases may achieve high protein yield, although the co-extraction of other components (i.e., phenolics) may also occur [25]. Besides glucanases, proteases are also used in EAE of SP, to achieve the release of proteins, peptides, and amino acids present in the supernatant of extracted samples [28].…”

Section: Enzyme-assisted Extractionmentioning

confidence: 99%

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Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification, and Applications

et al. 2021

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Seaweeds are industrially exploited for obtaining pigments, polysaccharides, or phenolic compounds with application in diverse fields. Nevertheless, their rich composition in fiber, minerals, and proteins, has pointed them as a useful source of these components. Seaweed proteins are nutritionally valuable and include several specific enzymes, glycoproteins, cell wall-attached proteins, phycobiliproteins, lectins, or peptides. Extraction of seaweed proteins requires the application of disruptive methods due to the heterogeneous cell wall composition of each macroalgae group. Hence, non-protein molecules like phenolics or polysaccharides may also be co-extracted, affecting the extraction yield. Therefore, depending on the macroalgae and target protein characteristics, the sample pretreatment, extraction and purification techniques must be carefully chosen. Traditional methods like solid–liquid or enzyme-assisted extraction (SLE or EAE) have proven successful. However, alternative techniques as ultrasound- or microwave-assisted extraction (UAE or MAE) can be more efficient. To obtain protein hydrolysates, these proteins are subjected to hydrolyzation reactions, whether with proteases or physical or chemical treatments that disrupt the proteins native folding. These hydrolysates and derived peptides are accounted for bioactive properties, like antioxidant, anti-inflammatory, antimicrobial, or antihypertensive activities, which can be applied to different sectors. In this work, current methods and challenges for protein extraction and purification from seaweeds are addressed, focusing on their potential industrial applications in the food, cosmetic, and pharmaceutical industries.

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Valorization of biomass for food protein via deep eutectic solvent extraction: Understanding the extraction mechanism and impact on protein structure and properties

Hanafi,

Anwar,

Saari

2024

Food Frontiers

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The conventional techniques for protein extraction from biomass are not fully aligned with sustainability goals, so it is important to look for some alternate solutions. By simultaneously extracting both soluble and insoluble proteins, deep eutectic solvents (DESs) offer a viable method for valorizing protein‐rich biomass from a variety of sources. Notably, the molecular crowding effects of DESs may have helped unfolded proteins acquire compact and stable conformations, facilitating solubilization and effective extraction. However, there is still a lack of information regarding how DESs interact with proteins and affect the structure and properties of the recovered proteins. To enable their widespread usage as a sustainable method for extracting dietary proteins, the safety of DES‐extracted proteins must also be addressed. In this paper, we review the state of the science in DES‐mediated protein extraction, focusing on the extraction mechanism and the interactions between DESs and proteins. Additionally, important aspects of DES‐mediated protein extraction that could affect the structure, technofunctional, nutritional characteristics, and safety of extracted protein are explored. DES‐based protein extraction could be helpful to valorize different biomasses for the production of food proteins due to the specific features.

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Effect of extraction condition on protein recovery and phenolic interference in Pacific dulse (Devaleraea mollis)

Cited by 17 publications

References 55 publications

Bacillus mycoides PM35 Reinforces Photosynthetic Efficiency, Antioxidant Defense, Expression of Stress-Responsive Genes, and Ameliorates the Effects of Salinity Stress in Maize

Bacillus mycoides PM35 Reinforces Photosynthetic Efficiency, Antioxidant Defense, Expression of Stress-Responsive Genes, and Ameliorates the Effects of Salinity Stress in Maize

Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification, and Applications

Valorization of biomass for food protein via deep eutectic solvent extraction: Understanding the extraction mechanism and impact on protein structure and properties

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