Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts

Vuong, Quan V.; Hirun, Sathira; Roach, Paul D.; Bowyer, Michael C.; Phillips, Phoebe A.; Scarlett, Christopher J.

doi:10.1016/j.hermed.2013.04.004

Cited by 265 publications

(250 citation statements)

References 26 publications

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“…Previous study [18] found that extraction yield of P. trimera root gained by water and methanol (11.48% and 11.04% by dry weight, respectively) were significantly higher than those obtained by acetonitrile, ethyl acetate and hexane (2.31%, 2.32% and 2.08% by dry weight, respectively), while the study of Rahman et al [23] demonstrated that phytochemicals and antioxidant potential of C. asiatica were affected by extraction solvent polarity in terms of 100% ethanol, 50% ethanol and water. In comparison with other materials, extraction yield of carrot peel was much higher than that of papaya leaf (19.15% by dry weight), as previously reported by Vuong et al [24], while the study of Chisté et al [25] found that the yield of solid extract from C. villosum fruit pulps obtained the highest level by mix of ethanol and ethyl acetate (1:1 v/v; 46.4% by dry weight), followed by ethyl acetate, water, ethanol and least by 50% ethanol (41.9%, 14.7%, 11.1% and 10.8% by dry weight, respectively). These results indicated that the extraction yield of carrot peel was greatly affected by solvents, of which methanol was the most effective solvent for extraction of solutes from carrot peel.…”

Section: Extraction Yieldsupporting

confidence: 75%

“…These data revealed that extraction efficiency of methanol relates to its intermediate polarity that allowed it to solvate low molecular weight organic compounds possessing protonatable functional groups, such as -COOH and -OH [18]. The study of Vuong et al [24] reported that TPC of papaya leaf extracted by various solvents was decreased in order of water, methanol, acetone and ethanol (23.06, 15.03, 10.71 and 9.43 mg GAE/g dry weight, respectively). Previous study of Shahriar et al [29] found that TPC of W. somnifera root was decreased in order by chloroform, petroleum ether, methanol, ethanol and n-hexane (60.99, 56.58, 23.86, 5.46 and 1.43 mg GAE/g dry weight, respectively).…”

Section: Total Phenolic Contentmentioning

confidence: 99%

“…Previous study of Shahriar et al [29] found that TPC of W. somnifera root was decreased in order by chloroform, petroleum ether, methanol, ethanol and n-hexane (60.99, 56.58, 23.86, 5.46 and 1.43 mg GAE/g dry weight, respectively). To compare with other plants, TPC of carrot peel was much higher than that of Sage plants [30] (approximately 2.3 mg GAE/g dry weight), but it was lower than those of Oolong tea [22] and papaya leaf [24] (13.58 and 15.03 mg GAE/g dry weight, respectively), and being much lower than those of P. amarus/urinaria/niruri [27] (50.24, 62.56 and 68.70 mg GAE/g dry weight, respectively). These results showed that the extractability of TPC from carrot peel was greatly affected by solvent character, and methanol was the most effective solvent for extraction of TPC from carrot peel.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

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Mass Proportion, Bioactive Compounds and Antioxidant Capacity of Carrot Peel as Affected by Various Solvents

Nguyen

Scarlett

2016

Technologies

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Abstract:The aim of this study was to determine the mass proportion of carrot root and the effects of four various solvents (methanol, water, ethanol and hexane) on the contents of total phenolics and saponins as well as antioxidant capacity of carrot peel to identify an optimal solvent for effective extraction of bioactive compounds from carrot peel for further investigation. The results showed that carrot root consisted of body, heads and peel with their mass proportion of 83.19%, 5.01% and 14.19% by fresh weight, respectively. Among four solvents tested, methanol obtained the highest levels of extraction yield (54.02% by dry weight), total phenolic content (9.02 mg GAE/g dry weight) and antioxidant capacity (DPPH radical scavenging capacity, cupric ion reducing antioxidant capacity, and ferric reducing antioxidant power) from carrot peel, while water extracted the highest content of saponins (272.9 mg EE/g dry weight) and possessed the maximum ABTS radical scavenging capacity. Therefore, methanol and water are considered for effective extraction of phenolics and saponins from carrot peel, respectively. The phenolic/saponin-enriched extracts are potential sources for further applications in the healthy food and/or pharmaceutical industries.

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Section: Extraction Yieldsupporting

confidence: 75%

Section: Total Phenolic Contentmentioning

confidence: 99%

Section: Total Phenolic Contentmentioning

confidence: 99%

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Mass Proportion, Bioactive Compounds and Antioxidant Capacity of Carrot Peel as Affected by Various Solvents

Nguyen

Scarlett

2016

Technologies

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“…Total phenolic content (TPC) was determined using a previously described method [6]. Briefly, 0.5 mL of diluted sample was mixed with 2.5 mL of Folin-Ciocalteu reagent 10% (v/v) and 2 mL of Na 2 CO 3 7.5% (w/v), then left in the dark at room temperature for 60 min.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

Characterising the Physical, Phytochemical and Antioxidant Properties of the Tuckeroo (Cupaniopsis anacardioides) Fruit

et al. 2017

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Abstract:The tuckeroo (Cupaniopsis anacardioides) is an Australian native plant that fruits over the summer months. There are very few studies that have characterised its fruit; consequently, this study aimed to delineate the physical, phytochemical and antioxidant properties of the tuckeroo fruit. The tuckeroo skin embodied the largest weight proportion with over 77% of the total fruit weight and it had the highest levels of total phenolic compounds (TPC; 151.36 mg GAE/g), total flavonoids compounds (TFC; 95.94 mg CAE/g), and proanthocyanidins (Proanth; 164.86 mg CAE/g) content, as well as the strongest antioxidant power. The seed and flesh accounted for 23% of the total fruit weight and they possessed significantly lower levels of TPC, TFC and Proanth. This study has demonstrated that the tuckeroo fruit skin is a rich source of phenolic compounds, which can be further isolated and identified for further utilisation in the food and pharmaceutical industries.

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“…Previous studies indicated that different solvents could lead to different extraction effi ciencies of bioactive compounds (Vuong et al, 2013;Zhang et al, 2013). Therefore, this study determined the impact of four different extraction solvents with various polarity indexes to identify the most effective solvent for further optimization using response surface methodology.…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Effect of extraction solvents on the biomolecules and antioxidant properties of Scorzonera undulata (Asteraceae): Application of factorial design optimization phenolic extraction

Athmouni

Belghith

Bellassouad

et al. 2015

Acta Sci Pol Technol Aliment

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Background. Phenolic compounds were extracted and isolated from S. undulata roots. Methods. Sample of roots from E. hirta was tested for phenolic compounds, and in vitro antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH) assay, ABTS, FRAP and reducing power was measured using cyanoferrate method. Results. The methanolic fraction exhibited the highest total phenol content (6.12 ±0.11 mg AGE/g DW). On the other hand, the highest fl avonoids concentration was observed in ethyl acetate fraction (2.90 ±0.05 mg CE/g DW) in addition to anthocyanins (28.56 ±3.96 mg/l). Besides, the highest level of tannins content was measured in the polar aprotic solvent ethyl acetate extract (3.25 ±0.06 mg CE/g DW). The different extracts of S. undulata were evaluated for their radical scavenging activities by means of the DPPH assay. The strongest scavenging activity was observed in methanolic fraction scavenged radicals effectively with IC 50 values of 0.14 ±0.02 mg/ml. Similarly, the potassium ferricyanide reduction (FRAP) and ABTS•+ of methanol extract. On the other hand, the total reducing power of ethyl acetate extract was found higher than of other extracts. This paper presents the application of the design-of experiment method for optimizing the extraction of phenolic content using methanol solvent. The resulting regression model has shown that the effect of temperature is not statistically signifi cant (with >95% certainty), while that of agitation speed is. The two main effects are contributed by the solvent concentration and the maceration period. Conclusion. Our results clearly showed that the extraction of phenolic compounds and their antioxidant capacity is signifi cantly affected by solvent combinations. S. undulata presented the highest total phenolic content, total fl avonoids content and antioxidant capacity values. The resulting regression model has shown that the effect of temperature is not statistically signifi cant (with >95% certainty), while that of agitation speed is.

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Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts

Cited by 265 publications

References 26 publications

Mass Proportion, Bioactive Compounds and Antioxidant Capacity of Carrot Peel as Affected by Various Solvents

Mass Proportion, Bioactive Compounds and Antioxidant Capacity of Carrot Peel as Affected by Various Solvents

Characterising the Physical, Phytochemical and Antioxidant Properties of the Tuckeroo (Cupaniopsis anacardioides) Fruit

Effect of extraction solvents on the biomolecules and antioxidant properties of Scorzonera undulata (Asteraceae): Application of factorial design optimization phenolic extraction

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