EFFECT OF EXTRACTION SOLVENT ON BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF Cichorium intybus L. GROWN IN KOSOVO

Abstract

The aim of this study was the investigation of the total phenolic content, flavonoid, and the assessment of the antioxidant potential of Cichorium intybus L. flowers grown in the Kosovo region. The flowers of Cichorium intybus L. were collected, dried, and extracted with solvents with different polarities (water, EtOH, MeOH, EtOAc, and acetone), using the extraction method. The total phenolic and flavonoid contents were analyzed by using Folin-Ciocalteu's and AlCl3 reagents, respectively. The antioxidant activity was assessed by DPPH in vitro assay methods. The obtained results revealed variation in the content of phenolic compounds (5.2 mg GAE/gDW in water extract, 6.8 mg GAE/gDW in ethanolic extract,72.1 0.3 mg GAE/gDW in methanolic extract, 45.3 0.1 mg GAE/g DW in ethyl acetate extract,and 31.3 0.1 mg GAE/gDW in acetone extract), flavonoid (2.9 mg CE/gDW in water extract, 19.0 mg CE/gDW in ethanolic extract,30.50 mg CE/gDW in methanolic extract, 27.1 mg CE/gDW in ethyl acetate extract, and 25.4 mg CE/gDW in acetone extract), and antioxidant activity (23.0 μMolTE/gDW in water extract, 124.7 μMolTE/gDW in ethanolic extract, 152.4 μMolTE/gDW in methanolic extract, 92.7 μMolTE/gDW in ethyl acetate extract,and 107.2 μMolTE/gDW in acetone extract). Cichorium intybus L. flowers from the Kosovo region are also a rich source of flavonoids. Further studies are recommended to quantify and isolate the pure phytoconstituents from Cichorium intybus L. flowers grown in the Kosovo region which might serve as natural antioxidants application in the food and drug industry.

Cichorium intybus Leaves Extract in Varying Solvent Systems for the Production of Platinum Nanoparticles: Design and Development of a Green Approach

“…Nevertheless, TPCs (mg GAE/100 g DW) were lowest in the de-ionized water extract (66.67). Comparing the phenolic contents of current study to earlier data reveals differences [49].…”

“…He con rmed that C. intybus has a very high avonoid content. The variations in avonoid levels observed in this study might potentially be attributed to variations in plant parts, extraction solvent system, and geographic variability [49]. Figure 6 shows the graphical comparison of Total avonoid contents (mg CE/g DW) C. intybus leaves varying solvents system.…”

Optimization of the Extraction Conditions of Antioxidant Phenolic Compounds from Strawberry Fruits (Fragaria x ananassa Duch.) Using Response Surface Methodology

“…Total phenolic contents of the extract of strawberry fruits were assessed by Folin-Ciocalteu reagent using the method previously reported by Singleton et al (1999) with slight modification, with the assistance of Folin-Ciocalteu reagent by Koraqi and Lluga-Rizani (2022). Briefly, 1 mL of each fruit extract diluted 1:20 with five different concentrations (12.5, 25, 50, 100, and 200 mg/mL) of each extract (in water, methanol, ethanol, acetonitrile, and acetone) was mixed with 1 mL distilled water and 500 μL Folin-Ciocalteu reagent and stirred for 1 min.…”

Environmentally Friendly Extraction of Bioactive Compounds from Rosa canina L. fruits Using Deep Eutectic Solvent (DES) as Green Extraction Media

“…Total phenolics content (TPC) was determined by Folin Ciocalteu Reagent spectrophotometrically at 765 nm using the method of Singleton et al (1999), 17 with some modifications reported by Koraqi and Lluga-Rizani (2022) 18 . The results are presented as mg gallic acid (mg GAE/g DW) equivalent per gram Rosa canina L. fruits sample.…”