Effect of extremely low‐frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544

Abstract

Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin injuries, we investigated whether significant cell damage occurs after ELF-EMF exposure. Reactive oxygen species (ROS) production was evaluated in the human keratinocyte exposed for 1 H to 50 Hz ELF-EMF in a range of field strengths from 0.25 to 2 G. Significant ROS increases resulted at 0.5 and 1 G and under these flux densities ROS production, glutathione content, antioxidant defense activity, and lipid peroxidation markers were assessed for different lengths of time. Analyzed parameters of antioxidant defense and membrane integrity showed a different trend at two selected magnetic fluxes, with a greater sensitivity of the cells exposed to 0.5 G, especially after 1 H. All significant alterations observed in the first 4 H of exposure reverted to controls 24 H after suggesting that under these conditions, ELF-EMF induces a slight oxidative stress that does not overwhelm the metabolic capacity of the cells or have a cytotoxic effect.

Role and mechanism of ROS scavengers in alleviating NLRP3‐mediated inflammation

“…Different cell lines used in researches is another factor that affects the results and conclusions. Even environment conditions like extremely low‐frequency electromagnetic fields are possible to impact on the antioxidant activity of cells . Compared with newly artificially synthesized antioxidants, natural or endogenous antioxidants might be less risky having unexpected impacts on patients or animals.…”

Expression levels of PDYN and OPRM1 genes in SH-SY5Y cells exposed to 50 Hz electromagnetic field

“…Previous studies have demonstrated that ELF-EMF is associated with production of ROS. [16][17][18] Moreover, reduced PDYN expression in the human brain is associated with opiate dependency. 35,36 Interestingly, in the present study the continuous exposure condition of ELF-EMF was associated with the lower expression levels of the PDYN.…”

“…15 It has been reported that ELF-EMF enhanced production of reactive oxygen species (ROS). [16][17][18] Cells maintain redox balance through production of ROS and cellular antioxidant capacity. The altered balance between generation and elimination of ROS plays a critical role in a variety of multifactorial complex traits including neurodegenerative diseases.…”

The Impact of 950mhz Electromagnetic Radiation on the Brain and Liver of Rats and the role of Garlic Treatment

“…et al, Demers et al, 2014). Headache, decreased learning potential, poor concentration, and oxidative stress were reported (Calcabrini et al, 2017 andAhmed et al, 2017).…”