Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells

Dou, Liping; Qu, Yan; Yang, Deyu

doi:10.3892/etm.2020.8444

Cited by 17 publications

(17 citation statements)

References 24 publications

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“…In this study, cellular analysis was performed using the eluates from both tested materials. According to the International Standard ISO 10993-12 Biological Evaluation of Medical Devices [17], we obtained eluates of the materials, and hPDLSCs were treated with different eluates, similar to previously reported studies [18][19][20]. The tests performed on those eluates allowed both qualitative and quantitative assessment of biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Liu

Hé

Wang

et al. 2021

J Mater Sci: Mater Med

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This study is aimed at comparing and evaluating the biocompatibility and antibacterial activities of mineral trioxide aggregate (MTA) and iRoot BP Plus as novel retro-filling materials. Discs of both materials were prepared and incubated for 72 h to obtain material extracts in medium. Flow cytometry and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used to assess the rate of apoptosis and proliferation of human periodontal ligament stem cells (hPDLSCs) when exposed to eluates of both materials. The expression levels of alkaline phosphatase, collagen type I, osteocalcin, Runt-related transcription factor-2, and Osterix were tested for evaluating the osteogenic differentiation of hPDLSCs. The antibacterial activities of both materials were compared by the direct contact test. The hPDLSCs stimulated by MTA or iRoot BP Plus eluates showed significantly higher cell viability than that of the control group with no eluates. No significant differences were observed among the percentages of necrotic and apoptotic cells stimulated by MTA and iRoot BP Plus eluates and the control group. The expression of all osteogenic differentiation markers of hPDLSCs in both experimental groups were significantly higher than those of the control group, while the increment values in MTA group were significantly higher than those of the iRoot BP Plus group. The antibacterial activity against Enterococcus faecalis showed no significant difference between MTA and iRoot BP Plus. Therefore, both materials may be suitable for retro-filling applications.

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Section: Discussionmentioning

confidence: 99%

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Liu

Hé

Wang

et al. 2021

J Mater Sci: Mater Med

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“…Similar studies have shown that a combination of Wnt3a, BMP7, and collagen gel delivered into the root canal of mini-pigs yielded pulp-dentin-like structures with obvious dentinal tubules (He et al, 2019). Furthermore, many bloodderived scaffold materials containing rich growth factors are used in clinical pulp therapy, such as platelet-rich plasma (PRP), concentrated growth factors, and platelet-rich fibrin, and showed a much better influence on cell proliferation, viability, apoptosis, and mineralization of human dental pulp cells than traditional materials such as Ca(OH) 2 , mineral trioxide aggregate, and iRoot BP (Dou et al, 2020). Moreover, Jadhav et al (2012) proved that PRP showed a better treatment effect in periapical healing, apical closure, and dentinal wall thickening compared to blood clots in regenerative endodontic tissue.…”

Section: Cytokines Combined With Scaffold Transplantationmentioning

confidence: 99%

Microenvironment Influences Odontogenic Mesenchymal Stem Cells Mediated Dental Pulp Regeneration

Huang

Liu

et al. 2021

Front. Physiol.

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Dental pulp as a source of nutrition for the whole tooth is vulnerable to trauma and bacterial invasion, which causes irreversible pulpitis and pulp necrosis. Dental pulp regeneration is a valuable method of restoring the viability of the dental pulp and even the whole tooth. Odontogenic mesenchymal stem cells (MSCs) residing in the dental pulp environment have been widely used in dental pulp regeneration because of their immense potential to regenerate pulp-like tissue. Furthermore, the regenerative abilities of odontogenic MSCs are easily affected by the microenvironment in which they reside. The natural environment of the dental pulp has been proven to be capable of regulating odontogenic MSC homeostasis, proliferation, and differentiation. Therefore, various approaches have been applied to mimic the natural dental pulp environment to optimize the efficacy of pulp regeneration. In addition, odontogenic MSC aggregates/spheroids similar to the natural dental pulp environment have been shown to regenerate well-organized dental pulp both in preclinical and clinical trials. In this review, we summarize recent progress in odontogenic MSC-mediated pulp regeneration and focus on the effect of the microenvironment surrounding odontogenic MSCs in the achievement of dental pulp regeneration.

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“…It is used for pulp capping, root perforation, retrograde root-end filling, and the obturation. 74 Review Article SMJ Biodentine. comprises tricalcium silicate, dicalcium silicate, calcium carbonate, calcium oxide, and zirconium oxide (radiopaque).…”

Section: Tri-calcium Silicate Based Materialsmentioning

confidence: 99%

Conceptualization of Bioactive Materials in Dental Caries Prevention

Govind

Jena²,

Mohanty

et al. 2021

Siriraj Med J

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Clinicians always face a challenge in selecting the appropriate material for clinical use. Presently, the term bioactive is being used judiciously and unquestionably in the field of dental materials. The introduction of nanotechnology brought about a revolution in material science leading to the development of bioactive materials for caries prevention and management. This review comprehensively evaluates the use of bioactive materials for modification of the oral biome, prevention of dental caries, and the management of dental caries as restorative materials. Six online database (PubMed, Scopus, Science Direct, Embase, Web of Science, and Cochrane library) were systematically searched using broad keywords. Published articles were scrutinized, analyzed and the full-text articles were selected. Data reveals relatively limited application of tissue engineering and regeneration for enamel and dentin due to their limited ability to remodel. However, many steps are being taken in biomimetic approach for the modification of dentin. The path to overcoming any challenges will require active collaboration among clinicians, a material scientist and pulp biologist.

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Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells

Cited by 17 publications

References 24 publications

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Microenvironment Influences Odontogenic Mesenchymal Stem Cells Mediated Dental Pulp Regeneration

Conceptualization of Bioactive Materials in Dental Caries Prevention

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