Effect of Five Polymorphisms on Percentage of Oleic Acid in Beef and Investigation of Linkage Disequilibrium to Confirm the Locations of Quantitative Trait Loci on BTA19 in Japanese Black Cattle

Abstract

Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.

The 84-bp Indel Polymorphism of The Sterol Regulatory Element-Binding Protein 1 (SREBP1) Gene in Several Cattle Breeds in Indonesia

“…SREBP1 or also known as sterol regulatory element binding transcription factor 1 (SREBF1) is also essential in the fat metabolism of ruminants because it also influences the fat characteristics of both meat and dairy products. This has been demonstrated in cattle (Harvatine & Bauman 2006;Proskura et al 2017;Gamarra et al 2021;Kawaguchi et al 2021), buffalo (Deng et al 2017;Xu et al 2019), goat (Xu et al 2016), and sheep (Tsiplakou et al 2015;Liang et al 2020;Carcangiu et al 2021). In bovine, the SREBP1 gene is located on chromosome 19 with 21 exons and encodes 1183 amino acids (Hoashi et al 2007).…”

Oleic acid concentration in bovine adipose tissues: impact on human health, sensory attributes, and genetic regulation

“…This region includes growth hormone (GH; 48.8 cM) but Taylor et al (1998) were unable to identify the other genes in BTA19 responsible for the differences in stearic and oleic acids. Kawaguchi et al (2021) reported fatty acid synthase (FASN; 51.4 cM) and sterol regulatory element-binding protein 1 (SREBP1; 35.2 cM) are located on BTA19. FASN regulates myristic acid and palmitic acid synthesis and SREPB1 is a transcription factor that regulates expression of FASN and SCD1 (located on BTA26; Campbell et al, 2001).…”