The rise of energy demand and ecological contaminations encourage the utilization of alcohol bases on substitutes fuels in spark ignition (SI) engine. The fusel oil is a byproduct acquired from fermentation process with higher alcohol content. It has high-octane and less exhaust emissions; therefore, it takes significant place between the substitutes fuels. During this study, the impact of utilizing mixtures of pure gasoline & fusel oil on engine exhaust emissions & performance has been evaluated after water extraction from fusel oil. A single-cylinder, four-stroke SI engine has been utilized in these tests. The examinations execute at different speeds. The experiment fuels mixed with fusel oil at proportions of 5%, 10%, 20%, and 30%. At each speed, the engine’s performance & emissions measurement have been conducted. During the experimentations, it has been noticed that the torque (T) and specified fuel consumption (SFC) rises as the quantity of fusel oil risen in the mixture. Carbon monoxide (CO) and carbon dioxide (CO2) emissions are decreased as the quantity of fusel oil is raised in mixtures.