Effect of functionalized polycaprolactone on the behaviour of murine preosteoblasts

Drevelle, Olivier; Bergeron, Éric; Senta, Helena; Lauzon, Marc-Antoine; Roux, Sophie; Grenier, Guillaume; Faucheux, Nathalie

doi:10.1016/j.biomaterials.2010.05.010

Cited by 30 publications

(25 citation statements)

References 36 publications

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“…This suggests that the anisotropic topographies conferred by uniaxial stretching could undergo proper modification for incorporating more desired physicochemical characteristics, such as RGD-peptides and CD34, on UX-PCL for particular application. 7,42 The anisotropic surface hydrophilicity observed on UX-PCL could be attributed by the increased surface roughness in perpendicular direction due to presence of the 3D micro-ridge/ grooves. 43 It is worthy to note that the anisotropy of surface hydrophilicity was retained during the hydrolysis treatment.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Three-Dimensional Anisotropic Geometries by Uniaxial Stretch of Poly(ɛ-Caprolactone) Films for Mesenchymal Stem Cell Proliferation, Alignment, and Myogenic Differentiation

Wang

Teo

Chong

et al. 2013

Tissue Engineering Part C: Methods

104

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Anisotropic geometries are critical for eliciting cell alignment to dictate tissue microarchitectures and biological functions. Current fabrication techniques are complex and utilize toxic solvents, hampering their applications for translational research. Here, we present a novel simple, solvent-free, and reproducible method via uniaxial stretching for incorporating anisotropic topographies on bioresorbable films with ambitions to realize stem cell alignment control. Uniaxial stretching of poly(ε-caprolactone) (PCL) films resulted in a three-dimensional micro-ridge/groove topography (inter-ridge-distance: ~6 μm; ridge-length: ~90 μm; ridge-depth: 200-900 nm) with uniform distribution and controllable orientation by the direction of stretch on the whole film surface. When stretch temperature (Ts) and draw ratio (DR) were increased, the inter-ridge-distance was reduced and ridge-length increased. Through modification of hydrolysis, increased surface hydrophilicity was achieved, while maintaining the morphology of PCL ridge/grooves. Upon seeding human mesenchymal stem cells (hMSCs) on uniaxial-stretched PCL (UX-PCL) films, aligned hMSC organization was obtained. Compared to unstretched films, hMSCs on UX-PCL had larger increase in cellular alignment (>85%) and elongation, without indication of cytotoxicity or reduction in cellular proliferation. This aligned hMSC organization was homogenous and stably maintained with controlled orientation along the ridges on the whole UX-PCL surface for over 2 weeks. Moreover, the hMSCs on UX-PCL had a higher level of myogenic genes' expression than that on the unstretched films. We conclude that uniaxial stretching has potential in patterning film topography with anisotropic structures. The UX-PCL in conjunction with hMSCs could be used as "basic units" to create tissue constructs with microscale control of cellular alignment and elongation for tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Biomimetic Three-Dimensional Anisotropic Geometries by Uniaxial Stretch of Poly(ɛ-Caprolactone) Films for Mesenchymal Stem Cell Proliferation, Alignment, and Myogenic Differentiation

Wang

Teo

Chong

et al. 2013

Tissue Engineering Part C: Methods

104

View full text Add to dashboard Cite

show abstract

“…2, left panel). In literature, to generate diverse surface chemistries, various post-modification methods for prefabricated polymer substrates have often been employed [16,[36][37][38]. The obvious disadvantages of post-modification lie in complex treatments and inherent heterogeneity, which might result in ambiguous observations in subsequent cell culture studies.…”

Section: Preparation Of Pcl Films Bearing Small Functional Groupsmentioning

confidence: 99%

Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells

Chen

Zhang

Zhou

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…It is known that the cell spreading event typically occurs through interaction of extracellular matrix (ECM) proteins (or their specic peptides) and their receptors. 36,37 Several studies, however, have demonstrated that osteoblast cell spreading can also be inuenced by immobilized calcium ions. 38,39 Because our experimental samples did not have any activating groups for cell spreading, such as ECM proteins or their specic peptides, we suspect that calcium ions attached to MAP via p-cation interaction fostered a better spreading environment than in the other control groups under serum-depleted conditions.…”

Section: 34mentioning

confidence: 99%

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

et al. 2015

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Effect of functionalized polycaprolactone on the behaviour of murine preosteoblasts

Cited by 30 publications

References 36 publications

Biomimetic Three-Dimensional Anisotropic Geometries by Uniaxial Stretch of Poly(ɛ-Caprolactone) Films for Mesenchymal Stem Cell Proliferation, Alignment, and Myogenic Differentiation

Biomimetic Three-Dimensional Anisotropic Geometries by Uniaxial Stretch of Poly(ɛ-Caprolactone) Films for Mesenchymal Stem Cell Proliferation, Alignment, and Myogenic Differentiation

Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

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