Effect of gamma rays and colchicine on silymarin production in cell suspension cultures of Silybum marianum: A transcriptomic study of key genes involved in the biosynthetic pathway

El-Garhy, Hoda A.S.; Sherif, Hassan S.A.; Soliman, Sara M.; Haredy, Shimaa A.; Bonfill, Mercedes

doi:10.1016/j.gene.2021.145700

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Also, Heydari et al [ 58 ] stated that low doses of γ-radiation favored callus growth in Salvia nemorosa , and a significant increase was found in the biomass growth of cultures that were subjected to 10 and 20 Gy doses. In a similar study, El-Garhy et al [ 59 ] concluded that γ-rays (200 and 600 Gy) and colchicine (0.05%) did not inhibit the formation of S. marianum L. calli and promoted cell culture growth. Thus, callus induction from leaf segments of seedlings obtained from seeds treated with γ-rays (200 and 600 Gy), 0.05% colchicine, or control conditions, showed high biomass production.…”

Section: Discussionmentioning

confidence: 96%

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Khalifa

Abd-ElShafy

Abu-Khudir

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background A useful technique for growing large amounts of plant material is in vitro propagation of important medicinal plants. The present investigation deals with the enhancement of secondary metabolite production via elicitation using gamma (γ)-radiation and phenylalanine (Phe) precursor feeding in callus cultures of Silybum marianum L. Results Seeds were exposed to two doses of γ-radiation (25 and 50 Gy) and the calli derived from stem explants obtained from seedlings of these radiated seeds were treated with different concentrations of Phe. The biosynthesis of phenols and flavonoids was evaluated. It was found that callus cultures derived from explants of the seeds exposed to 25 Gy γ-radiation and treated with 4 mg/l Phe accumulated the maximum phenolic content (34.27±0.02 mg/g d.wt.), while the highest flavonoid content (9.56±0.12 mg/g d.wt.) was found in callus cultures derived from explants of seeds radiated with 25 Gy γ-radiation and subjected to 1 mg/l Phe. Similarly, HPLC quantification revealed that the production of flavonoids was highly accumulated (1343.06 μg/mg d.wt.) in callus cultures from explants of seeds exposed to 25 Gy γ-radiation and grown at 1 mg/l Phe compared to the other treatments. In addition, a total of 11 important flavonoids have been determined in all callus cultures, except for acacetin-7-O-rutinoside, which was not found in the callus culture of the control. Conclusions These findings suggest that γ-radiation combined with Phe can improve the metabolism of S. marianum L. and could be used to produce such valuable metabolites on a commercial scale.

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Section: Discussionmentioning

confidence: 96%