Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

Wu, Chuancheng; Lu, Yuquan; Morimoto, Kanehisa

doi:10.1007/s12199-009-0126-5

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…Many life style factors such as nutrition, physical exercise, smoking, and alcohol consumption, in addition to age and gender, have previously been found to influence MN formation. 18,50,51 Here, we were able to link up to 60% of the observed fMN variation with the levels of 24 different adducts. The studied subcohort consists of only 51 individuals, though including the groups with highest and lowest fMN out of 150 individuals in the cohort.…”

Section: ■ Discussionmentioning

confidence: 99%

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Adductomic Screening of Hemoglobin Adducts and Monitoring of Micronuclei in School-Age Children

Carlsson

Aasa

Kotova

et al. 2017

Chem. Res. Toxicol.

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Electrophilic compounds/metabolites present in humans, originating from endogenous processes or exogenous exposure, pose a risk to health effects through their reactions with nucleophilic sites in proteins and DNA, forming adducts. Adductomic approaches are developed to screen for adducts to biomacromolecules in vivo by mass spectrometry (MS), with the aim to detect adducts corresponding to unknown exposures from electrophiles. In the present study, adductomic screening was performed using blood samples from healthy children about 12 years old (n = 51). The frequencies of micronuclei (MN) in erythrocytes in peripheral blood were monitored as a measure of genotoxic effect/genotoxic exposure. The applied adductomic approach has been reported earlier by us and is based on analysis of N-terminal valine adducts in hemoglobin (Hb) by liquid chromatography tandem mass spectrometry (LC-MS/MS). High resolution MS was introduced for refined screening of previously unknown N-terminal Hb adducts. Measured adduct levels were compared with MN frequencies using multivariate data analysis. In the 51 individuals, a total of 24 adducts (whereof 12 were previously identified) were observed and their levels quantified. Relatively large interindividual variations in adduct levels were observed. The data analysis (with partial least-squares regression) showed that as much as 60% of the MN variation could be explained by the adduct levels. This study, for the first time, applies the combination of these sensitive methods to measure the internal dose of potentially genotoxic chemicals and genotoxic effects, respectively. The results indicate that this is a valuable approach for the characterization of exposure to chemical risk factors for the genotoxic effects present in individuals of the general population.

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Section: ■ Discussionmentioning

confidence: 99%

“…Many life style factors such as nutrition, physical exercise, smoking, and alcohol consumption, in addition to age and gender, have previously been found to influence MN formation. ,, …”

Section: Discussionmentioning

confidence: 99%