Effect of genistein on semen quality, antioxidant capacity, caspase-3 expression and DNA integrity in cryopreserved ram spermatozoa

Elsayed, Doaa H.; El-Shamy, Ayat A.; Abdelrazek, Heba M. A.; El-Badry, Diya A.

doi:10.1016/j.smallrumres.2019.06.009

Cited by 12 publications

(16 citation statements)

References 49 publications

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“…Sperm motility and viability were increased following 50 μmol/L GEN addition and a subsequent incubation for 3 or 6 h. Furthermore, a beneficial impact of 50 μmol/L GEN was observed in case of the membrane integrity and mitochondrial activity, although it must be noted that these characteristics decreased in the presence of higher GEN concentrations (100 μmol/L). A similar observation was reported by Elsayed et al [ 235 ] who studied the impact of different GEN concentrations (0, 1, 5, 10 and 100 μmol/L) on the freezability and functional integrity of ram spermatozoa. The study reveals that high GEN levels (100 μmol/L) reduced the post thaw motility while low doses of the biomolecule (10 μmol/L) acted as an effective motility-promoting agent.…”

Section: Genisteinsupporting

confidence: 87%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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Emerging evidence from in vivo as well as in vitro studies indicates that natural biomolecules may play important roles in the prevention or management of a wide array of chronic diseases. Furthermore, the use of natural compounds in the treatment of male sub- or infertility has been proposed as a potential alternative to conventional therapeutic options. As such, we aimed to evaluate the effects of selected natural biomolecules on the sperm production, structural integrity, and functional activity. At the same time, we reviewed their possible beneficial or adverse effects on male reproductive health. Using relevant keywords, a literature search was performed to collect currently available information regarding molecular mechanisms by which selected natural biomolecules exhibit their biological effects in the context of male reproductive dysfunction. Evidence gathered from clinical trials, in vitro experiments and in vivo studies suggest that the selected natural compounds affect key targets related to sperm mitochondrial metabolism and motion behavior, oxidative stress, inflammation, DNA integrity and cell death. The majority of reports emphasize on ameliorative, stimulating and protective effects of natural biomolecules on the sperm function. Nevertheless, possible adverse and toxic behavior of natural compounds has been indicated as well, pointing out to a possible dose-dependent impact of natural biomolecules on the sperm survival and functionality. As such, further research leading to a deeper understanding of the beneficial or adverse roles of natural compounds is necessary before these can be employed for the management of male reproductive dysfunction.

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Section: Genisteinsupporting

confidence: 87%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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“…Further research into the effects of the addition of genistein to sperm has shown a reduction in motility and membrane integrity [ 84 ]. Similar to the observed beneficial effects reported in bovine semen, the addition of 10 µM of genistein to cryopreserved ram semen resulted in a significant increase in acrosomal integrity and post-thaw motility [ 85 ]. However at concentrations of 100 µM of genistein, there was a reduction in post-thaw motility, suggesting that higher concentrations of genistein may have deleterious effects upon the quality and functional integrity of the ram sperm [ 85 ].…”

Section: Effects On Male Reproductive Physiologymentioning

confidence: 59%

“…Similar to the observed beneficial effects reported in bovine semen, the addition of 10 µM of genistein to cryopreserved ram semen resulted in a significant increase in acrosomal integrity and post-thaw motility [ 85 ]. However at concentrations of 100 µM of genistein, there was a reduction in post-thaw motility, suggesting that higher concentrations of genistein may have deleterious effects upon the quality and functional integrity of the ram sperm [ 85 ]. Lower concentrations of genistein (<5 µM) were observed not to affect quality or integrity.…”

Section: Effects On Male Reproductive Physiologymentioning

confidence: 59%

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Wyse

Latif

Gurusinghe

et al. 2022

Animals

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Legume crops and pastures have a high economic value in Australia. However, legume species commonly used for grazing enterprises have been identified to produce high concentrations of phytoestrogens. These compounds are heterocyclic phenolic, and are similar in structure to the mammalian estrogen, 17β-estradiol. The biological activity of the various phytoestrogen types; isoflavones, lignans and coumestans, are species-specific, although at concentrations of 25 mg/kg of dry matter each of the phytoestrogen types affect reproductive functions in grazing livestock. The impacts upon fertility in grazing livestock such as cattle and sheep, vary greatly over length of exposure time, age and health of animal and the stress stimuli the plant is exposed to. More recently, research into the other effects that phytoestrogens may have upon metabolism, immune capacity and growth and performance of grazing livestock has been conducted. Potential new benefits for using these phytoestrogens, such as daidzein and genistein, have been identified by observing the stimulation of production in lymphocytes and other antibody cells. Numerous isoflavones have also been recognized to promote protein synthesis, increase the lean meat ratio, and increase weight gain in cattle and sheep. In Australia, the high economic value of legumes as pasture crops in sheep and cattle production enterprises requires proactive management strategies to mitigate risk associated with potential loss of fertility associated with inclusion of pasture legumes as forages for grazing livestock.

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“…Genistein ( 8 , 190 , 191 ) and myricetin ( 192 ) have been reported to cause premature capacitation and acrosome loss in human, boar, and mouse spermatozoa, and inhibit the acrosome reaction in bull spermatozoa. In contrast, other studies have reported that the inclusion of isoflavones in vitro improves sperm viability and function ( 193 – 195 ). One possible confounding factor is that several isoflavones, including the widely tested genistein, can act as an antioxidant and mitigate oxidative stress ( 196 , 197 ), thus improving sperm function.…”

Section: The Timing Of Phytoestrogen Exposure Is Critical For Reprodu...mentioning

confidence: 75%

Estrogenic Pastures: A Source of Endocrine Disruption in Sheep Reproduction

et al. 2022

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Phytoestrogens can impact on reproductive health due to their structural similarity to estradiol. Initially identified in sheep consuming estrogenic pasture, phytoestrogens are known to influence reproductive capacity in numerous species. Estrogenic pastures continue to persist in sheep production systems, yet there has been little headway in our understanding of the underlying mechanisms that link phytoestrogens with compromised reproduction in sheep. Here we review the known and postulated actions of phytoestrogens on reproduction, with particular focus on competitive binding with nuclear and non-nuclear estrogen receptors, modifications to the epigenome, and the downstream impacts on normal physiological function. The review examines the evidence that phytoestrogens cause reproductive dysfunction in both the sexes, and that outcomes depend on the developmental period when an individual is exposed to phytoestrogen.

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Effect of genistein on semen quality, antioxidant capacity, caspase-3 expression and DNA integrity in cryopreserved ram spermatozoa

Cited by 12 publications

References 49 publications

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

Phytoestrogens: A Review of Their Impacts on Reproductive Physiology and Other Effects upon Grazing Livestock

Estrogenic Pastures: A Source of Endocrine Disruption in Sheep Reproduction

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