Effect of Grape Seed Polyphenol on immune gene expression and it's Role as Antibacterial against Salmonella typhimurium infection in mice exposed to sodium nitrate

Abstract

Polyphenols especially in grape seeds have anti-inflammatory effects by immunomodulation and anti-oxidantive pathway ,as well as antimicrobial activities .The current research conducted to evaluate the immunomodulatory effect Grape Seed Polyphenol extracts (GSP) against Salmonella Typhimurium infection and fed sodium nitrate in mice.The parameters which were used in this study, including determention the bacterial clearance duration in liver tissue by counting the bacterial colony forming unit(CFU)/gram and detecting the gene expression of Tumer nicrosis facter (TNFα) and interleukin-10 (IL-10) in liver tissue by the using quantitative real time reverse transcription PCR (RT-qPCR )technique . The log 10 CFU/liver count was demonstrated at days 5 ,10and 15 after challenge; the treated groups recorded low bacterial count with a significant difference (p < 0.01 ) in GSP treated groups due to the activated innate immunity was an important key to activate the protective Th1 responses through a significant decrease in the bacterial liver count after treated .The results obtained from the gene expression in mice treated with GSP , showed a lower values of TNFα mRNA in liver tissue .While IL-10 mRNA showed significant (P < 0.05) increase .

Ethyl acetate extract of Clausena execavatapromotes growth inhibition of Burkitt’s lymphoma cell line via apoptotic activities

“…It was also suggested that phenolic compounds inhibit the stress-activated NF-κβ and AP-1 signaling cascade (Albaayit et al, 2020b). Phenolic compounds also enhance the immune system in the destruction of cancer cells, inhibit angiogenesis, and reduce the metastatic potential of tumors (Mohammad & Mahdi, 2017;Atiyah & Kadhum, 2021). Excavatine A, carbazole alkaloid is reported for its anticancer activity against alveolar basal epithelial cell and cervical cancer (Peng et al, 2013a).…”