Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb

Development of an enzyme-linked immunosorbent assay for diniconazole in agricultural samples

“…The number of hapten molecules per molecule of protein (hapten density) for the each conjugate was estimated directly from the molar absorptivity at 280 nm (e 280 ) (Zhang et al, 2008): Hapten density ¼ ðe conjugation À e protein Þ=e hapten…”

An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody

“…To generate immunogens, haptens 3 and 4 were covalently attached through their carboxylic acid moieties to the lysine groups of BSA using the active ester method Zhang, Wu et al, 2008;Liang, Liu, Liu, Yu, & Fan, 2008;Ju, Tang, Fan, & Chen, 2008;Lee, Kim, Park, Chung, & Lee, 2005). Briefly, Haptens were activated as a result of treatment with a 50% molar excess of NHS and DCC for 6 h at room temperature.…”

A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos

“…The conjugation HTCP-HRP was synthesized according to the previous literature with a slight modification (Qian et al, 2009;Zhang et al, 2008). All of the HTCP, NHS, EDC were diluted into DMF.…”