Effect of hydrogen peroxide on Microcystic aeruginosa: Role of cytochromes P450

Wang, Jia; Chen, Zunwei; Chen, Hui; Wen, Yuezhong

doi:10.1016/j.scitotenv.2018.01.067

Cited by 30 publications

(25 citation statements)

References 47 publications

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“…Several studies found that cells open and die upon H 2 O 2 exposure, as shown by SYTOX staining of cells with damaged cell membranes [58], electron microscopy [68,82,83] and measurement of K + release [83], as well as caspase-3-like activity as a trigger for programmed cell death [82,83]. Perforation of the cell membrane would also explain the release of intracellular MC, as observed in our study and several previous studies [67,84,85].…”

Section: Damage Caused By H 2 Osupporting

confidence: 84%

Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities

Piel

Sandrini

White

et al. 2019

Toxins

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Hydrogen peroxide (H2O2) can be used as an emergency method to selectively suppress cyanobacterial blooms in lakes and drinking water reservoirs. However, it is largely unknown how environmental parameters alter the effectiveness of H2O2 treatments. In this study, the toxic cyanobacterial strain Microcystis aeruginosa PCC 7806 was treated with a range of H2O2 concentrations (0 to 10 mg/L), while being exposed to different light intensities and light colors. H2O2 treatments caused a stronger decline of the photosynthetic yield in high light than in low light or in the dark, and also a stronger decline in orange than in blue light. Our results are consistent with the hypothesis that H2O2 causes major damage at photosystem II (PSII) and interferes with PSII repair, which makes cells more sensitive to photoinhibition. Furthermore, H2O2 treatments caused a decrease in cell size and an increase in extracellular microcystin concentrations, indicative of leakage from disrupted cells. Our findings imply that even low H2O2 concentrations of 1–2 mg/L can be highly effective, if cyanobacteria are exposed to high light intensities. We therefore recommend performing lake treatments during sunny days, when a low H2O2 dosage is sufficient to suppress cyanobacteria, and may help to minimize impacts on non-target organisms.

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Section: Damage Caused By H 2 Osupporting

confidence: 84%

Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities

Piel

Sandrini

White

et al. 2019

Toxins

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“…and other cyanobacteria represents a threat to drinking water and the use of recreational lakes worldwide (Carmichael &Boyer 2016, O'neil et al 2012. H 2 O 2 application is an effective anti-cyanobacterial control method (Matthijs et al 2012, Wang et al 2018. H 2 O 2 has a short life span of 4 h to 20 h in water bodies (Cooper et al 1994) and is selectively toxic towards cyanobacteria compared to other phytoplankton taxa and aquatic invertebrates (Jančula et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of H2O2 on growth, metabolic activity and membrane integrity in three strains of Microcystis aeruginosa

Foo

Chapman

Hartnell

et al. 2020

Environ Sci Pollut Res

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The application of hydrogen peroxide (H 2 O 2 ) as a management tool to control Microcystis blooms has become increasingly popular due to its short lifetime and targeted action. H 2 O 2 increases intracellular reactive oxygen species resulting in oxidative stress and subsequently cell death. H 2 O 2 is naturally produced in freshwater bodies as a result of photocatalytic reactions between dissolved organic carbon and sunlight. Previously, some studies have suggested that this environmental source of H 2 O 2 selectively targets for toxigenic cyanobacteria strains in the genus Microcystis. Also, past studies only focused on the morphological and biochemical changes of H 2 O 2 -induced cell death in Microcystis with little information available on the effects of different H 2 O 2 concentrations on growth, esterase activity and membrane integrity. Therefore, this study investigated the effects of non-lethal (40-4000 nM) concentrations on percentage cell death; with a focus on sub-lethal (50 μM) and lethal (275 μM; 500 μM) doses of H 2 O 2 on growth, cells showing esterase activity and membrane integrity. The non-lethal dose experiment was part of a preliminary study. Results showed a general effect of dose and time dependent relationship in all three Microcystis strains post H 2 O 2 treatment. H 2 O 2 resulted in a significant increase in intracellular reactive oxygen species, decreased chlorophyll a content, decreased growth rate and esterase activity. Interestingly, at sub-lethal (50 µM H 2 O 2 treatment), percentage of dead cells in microcystin-producing strains were significantly higher (p<0.05) from non-microcystin producing strains at 72h. These findings further cement our understanding of the influence of H 2 O 2 on different strains of Microcystis and its impact on membrane integrity and metabolic physiology; important to future toxic bloom control programmes.

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“…In this study, a range of methods for identifying cell stress in the cyanobacterium Microcystis aeruginosa PCC 7813 under the effect of different concentrations of H2O2 were compared to identify which analytical method provides the most accurate and rapid response. Further, although several studies have analyzed the effects of H2O2 on MC-LR (Qian et al, 2010;Matthijs et al, 2012;Papadimitriou et al, 2016;Kansole and Lin, 2017;Chang et al, 2018;Fan et al, 2019;Wang et al, 2015Wang et al, , 2018Wang et al, , 2019, here, for the first time, the degradation of intra-and extracellular concentration of four different microcystin analogues (MC-LR, MC-LF, MC-LY, and MC-LW) was evaluated under different concentrations of H2O2.…”

mentioning

confidence: 99%

Oxidative stress in the cyanobacterium Microcystis aeruginosa PCC 7813: Comparison of different analytical cell stress detection assays

et al. 2021

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Effect of hydrogen peroxide on Microcystic aeruginosa: Role of cytochromes P450

Cited by 30 publications

References 47 publications

Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities

Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities

Effects of H2O2 on growth, metabolic activity and membrane integrity in three strains of Microcystis aeruginosa

Oxidative stress in the cyanobacterium Microcystis aeruginosa PCC 7813: Comparison of different analytical cell stress detection assays

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