Effect of hyperlipidemic serum on lipid peroxidation, synthesis of prostacyclin and thromboxane by cultured endothelial cells: Protective effect of antioxidants

Wang, Jian; Zhen, Erzhen; Guo, Zhenhui; Lu, Yongcai

doi:10.1016/0891-5849(89)90131-7

Cited by 58 publications

(15 citation statements)

References 23 publications

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“…Superoxide reacts with nitric oxide to form peroxynitrite; so increased peroxynitrite suggests an endothelial deficiency of nitric oxide. Both of these deficiencies could be due to the infiltration of neutrophils because ROS inhibit prostacyclin synthase 25, 26 and superoxide specifically reacts with nitric oxide to form peroxynitrite. To evaluate the role of endothelial factors, we compared vascular response in endothelium intact and endothelium denuded vessels.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Enhanced Vascular Reactivity in Preeclampsia

et al. 2011

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Preeclamptic women have enhanced blood pressure response to angiotensin II and extensive systemic vascular infiltration of neutrophils. Neutrophils release reactive oxygen species that might activate the RhoA kinase pathway to enhance vascular reactivity. We hypothesized that enhanced vascular reactivity in preeclampsia is due to neutrophil mediated reactive oxygen species activation of the RhoA kinase pathway. Omental arteries were obtained at cesarean section and studied using a myograph system. We found that arteries of preeclamptic women had extensive infiltration of neutrophils and enhanced reactivity to angiotensin II. Treatment of arteries of normal pregnant women with reactive oxygen species or activated neutrophils enhanced vessel reactivity to angiotensin II mimicking preeclamptic vessels. Pretreatment with superoxide dismutase/catalase to quench reactive oxygen species, or RhoA kinase inhibitor blocked enhanced responses in preeclamptic and normal vessels. Reactive oxygen species also enhanced vessel reactivity to norepinephrine, which was blocked by RhoA kinase inhibition. Treatment of arteries with reactive oxygen species increased RhoA kinase activity 3-fold, whereas culture of human vascular smooth muscle cells with angiotensin II and activated neutrophils or reactive oxygen species resulted in phosphorylation of key proteins in the RhoA kinase pathway. We conclude that enhanced vascular reactivity of omental arteries in preeclampsia is due to reactive oxygen species activation of the RhoA kinase pathway, and that enhanced vascular reactivity is likely due to the infiltration of neutrophils. We speculate that neutrophil infiltration into systemic vasculature of preeclamptic women is an important mechanism for hypertension.

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Section: Discussionmentioning

confidence: 99%

Mechanisms of Enhanced Vascular Reactivity in Preeclampsia

et al. 2011

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“…The imbalance between thromboxane and prostacyclin is most likely caused by oxidative stress, which is manifest in PIH by increased lipid peroxidation and decreased antioxidant protection 155. Abnormally increased lipid peroxides in the maternal plasma and the placenta could activate the COX enzyme to increase thromboxane synthesis,157 but at the same time, they inhibit prostacyclin synthase to decrease prostacyclin production 158. Interestingly, pre‐eclamptic plasma was shown to activate NF‐ κ B159 in endothelial cells, while NF‐ κ B is an oxidant‐sensitive nuclear factor that could induce transcription of many mediators of inflammation, including COX‐2 146.…”

Section: Physiological Importance Of Fatty Acids On Pregnancy Outcomementioning

confidence: 99%

Effect of Placental Fatty Acid Metabolism and Regulation by Peroxisome Proliferator Activated Receptor on Pregnancy and Fetal Outcomes

Wang

Cook

et al. 2007

Journal of Pharmaceutical Sciences

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“…Several studies also demonstrated the protective effect of selenium against free radical-induced tissue damage (e.g., heart, liver, endothelial cells) [3,15,29,37,39] in vivo. Selenium has also been shown to reduce the renal tubular toxicity of cis-platinum [2,5,20] which is also caused by the increased formation of reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

Selenium reduces high energy shock wave-induced renal injury in rats

Strohmaier

2002

Urological Research

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Using an in vitro model with Madin-Darby canine kidney (MDCK) cells, we showed that shock wave-induced renal injury could be ameliorated by selenium. We examined the influence of selenium, a free radical scavenger, in shock wave-induced tubular cell injury in vivo. Male rats were randomly assigned to three groups: 1 control (n= 18), 2 selenium (n = 18), 3 sham treatment (n = 4). Groups 1 and 2 were treated with 500 shock waves on each kidney. Animals assigned to group 3 (sham treatment) received only anesthetics. Selenium (80 microg/kg per 24 h intraperitoneally) was given to the animals in group 2 for 5 days, starting 1 day before shock wave exposure. Urine was collected for 8 h on the day before and immediately, 1, 7 and 28 days after shock wave exposure (SWE) for the measurement of urine volume, N-acetyl-beta-glucosaminidase (NAG), beta-2-microglobulin (beta2 M), and creatinine. Blood was taken from these rats on day 1 after SWE for the determination of creatinine and the calculation of the creatinine clearance (CCr). After SWE, there was a significantly increased diuresis in group 1 and 2. The excretion of NAG and beta2 M was also increased in both groups. These changes were significantly less pronounced in the selenium treated rats. CCr was higher in the selenium group than in the controls. No changes were observed in the sham treated group. These results demonstrate that selenium is able to ameliorate the damaging effects of high energy shock waves on renal tissue not only in vitro, but also in vivo.

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Effect of hyperlipidemic serum on lipid peroxidation, synthesis of prostacyclin and thromboxane by cultured endothelial cells: Protective effect of antioxidants

Cited by 58 publications

References 23 publications

Mechanisms of Enhanced Vascular Reactivity in Preeclampsia

Mechanisms of Enhanced Vascular Reactivity in Preeclampsia

Effect of Placental Fatty Acid Metabolism and Regulation by Peroxisome Proliferator Activated Receptor on Pregnancy and Fetal Outcomes

Selenium reduces high energy shock wave-induced renal injury in rats

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