Effect of immunization against GnRH on hypothalamic and testicular function in rams

Han, Xingfa; Gu, Long-Jun; Xia, Chen; Feng, Jing; Cao, Xiaohan; Du, Xiaogang; Zeng, Xianyin; Song, Tianzeng

doi:10.1016/j.theriogenology.2014.10.029

Cited by 27 publications

(34 citation statements)

References 36 publications

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“…Serum anti-GnRH titers were determined by an RIA as our previous descriptions [15]. Namely, GnRH was labeled with 125 I using the chloramine T method.…”

Section: Serum Anti-gnrh Antibody Titersmentioning

confidence: 99%

“…Serum testosterone, LH and FSH concentrations were assayed exactly as our previously described [15]. Serum 17β-estradiol concentrations were determined using commercial kits for sheep (ELISA), according to the manufacturer's instructions (Cusabio Biotech CO., Ltd., Wuhan, China).…”

Section: Serum Reproductive Hormone Profilesmentioning

confidence: 99%

“…Active immunization of animals against GnRH (also called immunocastration) neutralizes endogenous GnRH and causes loss of both pituitary LH, FSH and gonadal steroids, and terminates gametogenesis, which has been applied as an animal friendly alternative to surgical castrations in animal husbandry [15,16]. In GnRH-immunized animals, the neuroendocrine function of HPG axis is blocked in short or long time [17].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, there is an urgent need for alternative methods. Accordingly, we conducted GnRH vaccination researches in Tibetan rams and demonstrated that immunocastration could as an effective alternative to surgical castration for Tibetan rams [15]. However, Tibetan plateau possesses an extremely harsh natural environment which has profound effects on animal survival [21].…”

Section: Introductionmentioning

confidence: 99%

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Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

Han

Xiao-hua

Zeng

et al. 2016

International Immunopharmacology

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“…Serum anti-GnRH titers were determined by an RIA as our previous descriptions [15]. Namely, GnRH was labeled with 125 I using the chloramine T method.…”

Section: Serum Anti-gnrh Antibody Titersmentioning

confidence: 99%

Section: Serum Reproductive Hormone Profilesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

Han

Xiao-hua

Zeng

et al. 2016

International Immunopharmacology

Self Cite

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“…No presente estudo, a redução na qualidade seminal foi possivelmente devido a redução nos níveis circulantes de testosterona, pois a testosterona tem importante papel nos estágios finais da produção espermática (HAN et al, 2015). AmATmYAkul-cHANTler et al (2013) 10,4 ± 5,04 7,7 ± 4,80 3,5 ± 1,69 1,8 ± 2,30 2 14,0 ± 3,08 11,6 ± 7,70 4,0 ± 2,39 3,3 ± 0,57 3,5 ± 2,82 3 11,8 ± 4,13 8,8 ± 5,58 5,4 ± 4,97 2,0 ± 2,94 5,1 ± 1,57 4 9,8 ± 3,41 7,2 ± 1,27 7,0 ± 5,95 3,7 ± 1,70 6,6 ± 3,50 5 23,0 ± 10,07 6,7 ± 4,22 9,7 ± 3,63 3,5 ± 2,12 10,2 ± 2,98 6 17,0 ± 8,49 7,5 ± 3,53 10,6 ± 5,94 6,2 ± 1,02 13,8 ± 2,67…”

Section: Resultsunclassified

Efeito da imunocastração sobre o perímetro escrotal e a produção espermática em touros da raça nelore

2017

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EFFECT OF IMMUNOCASTRATION ON SCROTAL CIRCUMFERENCE AND SPERM PRODUCTION OF NELLORE BULLSABSTRACT: Bopriva® exerts anti-gonadotropin-releasing hormone (GnRH) activity and is used for immunocastration of cattle. The objective of this study was to evaluate the effect of Bopriva® on scrotal circumference and sperm production and quality of Nellore bulls. Five Nellore bulls (Bos taurus indicus), aged 12 months, received two doses of Bopriva® at an interval of 30 days, with each dose of 1 mL/animal containing 400 μg of the product. At 2-weeks intervals, scrotal circumference was measured and semen was collected by electroejaculation (seven samplings) and submitted to physical and morphological analysis. Scrotal circumference significantly decreased after the 4th sampling (P=0.0056) and sperm motility ranged from 20% to 66% (mean of 39%), sperm vigor from 1.0 to 2.8 (mean of 1.74), and sperm concentration from 87.8 to 296.2 million spermatozoa per mL (mean of 242.9 million). The percentage of total sperm defects ranged from 36% to 75.6% (mean of 49.51%), with a high incidence of cytoplasmic droplets, bent tails and detached heads. Bopriva® reduces scrotal circumference and interferes with semen quality in Nellore animals, reducing sperm motility and increasing the percentage of sperm abnormalities in the ejaculate.

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Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

Minhas

Shrestha

Wadhwa

et al. 2016

Molecular Reproduction Devel

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Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17 or TT-KK-Sp17 , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli. Immunization of female FVB/J mice, using alum as an adjuvant, led to the generation of high antibody titers against the above proteins. Antibodies against both N- and C-terminal fragments of Sp17 reacted with the entire capacitated mouse spermatozoa, whereas those against equatorin reacted exclusively with the equatorial region. Despite the reactivity of all immune sera, only sera from mice immunized with TT-KK-Sp17 and TT-KK-Sp17 significantly reduced mouse in vitro fertilization. Mating studies of the immunized females with un-immunized male mice revealed the highest infertility in the TT-KK-Sp17 -immunized group. In an attempt to further boost the immune response, the C-terminal fragment of Sp17 was expressed as fusion protein with a tandem repeat of gonadotropin-releasing hormone (GnRH) (Sp17 -GnRH ). Immunization of both male and female mice with Sp17 -GnRH led to higher contraceptive efficacy compared to mice immunized with TT-KK-Sp17 . Interestingly, mating studies wherein partners were both immunized with Sp17 -GnRH showed a complete failure of female mice to conceive. Thus, immunization of both males and females with Sp17 -GnRH has the potential to increase contraceptive efficacy. Mol. Reprod. Dev. 83: 1048-1059, 2016. © 2016 Wiley Periodicals, Inc.

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Effect of immunization against GnRH on hypothalamic and testicular function in rams

Cited by 27 publications

References 36 publications

Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

Efeito da imunocastração sobre o perímetro escrotal e a produção espermática em touros da raça nelore

Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

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