Effect of in vitro selected synbiotics (galactooligosaccharide and mannanoligosaccharide with or without Enterococcus faecalis) on growth performance, immune responses and intestinal microbiota of juvenile narrow clawed crayfish, Astacus leptodactylus lep

Safari, Omid; Paolucci, Marina

doi:10.1111/anu.12553

Cited by 22 publications

(21 citation statements)

References 65 publications

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“…Dietary supplementation of feed additives have significant impacts on growth, immunity and disease resistance of crustaceans 3,7,9,29,[37][38][39][40] . Probiotic in aqua diets, has positive influence on the growth and immune performance of commercially farmed shrimp and crayfish species.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Foysal

Fotedar

Siddik

et al. 2020

Sci Rep

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This study aimed to investigate the combined effects of two most potent probiotic bacteria Lactobacillus acidophilus and Lactobacillus plantarum on overall health and immune status of freshwater crayfish, marron under laboratory conditions. A total of 36 marron were distributed into six different tanks and two different feeding groups, control and probiotic-fed group. After acclimation, control group was fed with basal diet while probiotic group was fed 10 9 cfU/mL per kg of bacterial supplemented feed for 60 days. The results showed no significant differences in weight gain, however, probiotic feed significantly enhanced some hemolymph parameters and biochemical composition of tail muscle. Histology data revealed better hepatopancreas health and higher microvilli counts in the marron gut fed probiotic diet. The probiotic bacteria triggered significant shift of microbial communities at different taxa level, mostly those reported as beneficial for crayfish. The probiotic diet also enriched the metabolic functions and genes associated with innate immune response of crayfish. Further correlation analysis revealed significant association of some taxa with increased activity for hemolymph and immune genes. therefore, dietary Lactobacillus supplementation can modulate the overall health and immunity as well as gut microbial composition and interaction network between gut microbiota and immune system in crayfish. Aquaculture has become an important food sector for meeting the overall protein demand for growing population. The global consumption of crustaceans has increased greatly and especially those with live transport abilities are increasingly becoming popular 1-3. Marron (Cherax cainni) is one of the largest freshwater crayfish farmed in Western Australia (WA) that has high nutritive value and widespread consumer preferences 4,5. In addition, long distance live transportation ability of marron further increases its international demand, and thus has become an ideal crayfish species for commercial farming 6,7. However, the production of marron in WA has remained stagnant for a long time 7. Selection of proper diets and maintaining the optimum water quality are the two most crucial factors required for marron farming 5,7. Although no disease outbreaks have yet been reported, the interest and intend of intensification in marron farming can expose marron to possible crayfish pathogens including Vibrio, Aeromonas and Rhodobacter 5,6. In the past, several laboratory based trials have been conducted 3,6-9 , however, finding a suitable diet and identifying the beneficial bacteria that potentially can influence the growth and immune performance of marron has still remained unknown. Towards sustainable development of aquaculture, the use of feed additives including probiotics, prebiotics, synbiotics, parabiotics and phytogenics in crustacean's diet to boost biological indices has gained extensive attention from the researchers and farmers. Probiotics are microorganisms associated with health and immune benefits for the host ...

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Section: Discussionmentioning

confidence: 99%

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Foysal

Fotedar

Siddik

et al. 2020

Sci Rep

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show abstract

“…The intestine was removed, rinsed using distilled water, dried with paper towels, homogenized with 30 g/70 ml distilled water using a homogenizer (DI 18 Disperser). Then, the samples were centrifuged at 10,000 g for 25 min at 4°C (Safari & Paolucci, ), and the supernatant was stored in liquid nitrogen. All intestine tissues were collected one by one without pooling.…”

Section: Methodsmentioning

confidence: 99%

“…One‐way analysis of variance (ANOVA) followed by Duncan post hoc was used to characterize significant difference among various groups as p < 0.05. All statistical analyses were carried out using IBM SPSS Statistics, version 23 (Safari & Paolucci, ).…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation effects ofPediococcus acidilacticias probiotic on growth performance, digestive enzyme activities and immunity response in zebrafish (Danio rerio)

et al. 2019

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This study was carried out to assess the effects of Pediococcus acidilactici on zebrafish (Danio rerio). Different levels of P. acidilactici including 0, 1 × 106, 2 × 106, 4 × 106 and 8 × 106 colony‐forming unit per g of the diet (cfug−1) were examined in fish with 120 ± 10 mg weight for 60 days in a completely randomized design. The results showed that the best growth indices were recorded in group 4 × 106 cfug−1 (p < 0.05). The highest number of total viable count and lactic acid bacteria of intestine were found in group 4 × 106 cfug−1 (p < 0.05). The maximum activity of digestive enzymes including amylase, lipase, protease and alkaline phosphatase was observed in group 4 × 106 cfug−1. The highest activity for superoxide dismutase was recorded in group 4 × 106 cfug−1 while catalase, glutathione peroxidase and glutathione reductase showed the highest activity in group 8 × 106 cfug−1. The most growth inhibition zone of Aeromonas hydrophylla, Flavobacterium columnare, Vibrio anguillarum and Edwardsiella tarda was found in group 4 × 106 cfug‐1 (p < 0.05). Therefore, P. acidilactici as a probiotic improved growth and immunity of the zebrafish and could be used by zebrafish farmers.

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“…For these reasons, the selection of a prebiotic capable of promoting the growth of butyrate-producing bacteria is necessary to enhance survival rate and improve aquaculture production. Supplementation with prebiotics might be a promising method that could confer positive health benefits in aquatic animals, including cultured crustaceans (Daniels et al, 2013;Chen et al, 2017;Duan et al, 2017;Elshopakey et al, 2018;Safari and Paolucci, 2018). In vitro anaerobic fermentation studies provide a primary approach for initial evaluation of the positive benefits of compounds through their effects on metabolic activities of gut microbiota (Pham et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Galactooligosaccharides and Resistant Starch Altered Microbiota and Short-Chain Fatty Acids in an in vitro Fermentation Study Using Gut Contents of Mud Crab (Scylla paramamosain)

Tran

Tang

et al. 2020

Front. Microbiol.

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Dietary carbohydrates are anaerobically fermented by gut microbiota to shortchain fatty acids (SCFAs), conferring gut health benefits. Of all tested prebiotics, galactooligosaccharides (GOS) and resistant starch (RS) stimulated the SCFA production in mud crab (Scylla paramamosain), a crustacean model, to a greater extent than the other carbohydrates tested. Using in vitro anaerobic fermentation cultures, this study further explored the prebiotic potential of GOS and RS in mud crab by assessing their impacts on gut microbiota changes and SCFA production. Both GOS and RS significantly promoted SCFA production. Bacterial diversity in the GOS group was lower than in the RS or control group. GOS promoted the growth of Bacteroidetes, while RS promoted Tenericutes. A strong positive correlation was found between SCFA production and bacterial abundance; most bacteria per se correlated with each other. The findings demonstrated the prebiotic potential of GOS and RS in mud crab.

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Effect of in vitro selected synbiotics (galactooligosaccharide and mannanoligosaccharide with or without Enterococcus faecalis) on growth performance, immune responses and intestinal microbiota of juvenile narrow clawed crayfish, Astacus leptodactylus lep

Cited by 22 publications

References 65 publications

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Dietary supplementation effects ofPediococcus acidilacticias probiotic on growth performance, digestive enzyme activities and immunity response in zebrafish (Danio rerio)

Galactooligosaccharides and Resistant Starch Altered Microbiota and Short-Chain Fatty Acids in an in vitro Fermentation Study Using Gut Contents of Mud Crab (Scylla paramamosain)

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