Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization

Delabona, Priscila da Silva; Pirota, Rosangela Donizete Perpetua Buzon; Codima, Carla Aloia; Tremacoldi, C. R.; Rodrigues, André; Farinas, Cristiane S.

doi:10.1016/j.indcrop.2012.05.035

Cited by 102 publications

(65 citation statements)

References 28 publications

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“…This results contradicts the results published by Andrade et al (2011) and Delabona et al (2013), who reported optimum pH values at 3.0 for cellulases produced by A. fumigatus and Trichoderma sp. IS-05, respectively.…”

Section: Effect Of Temperature and Ph On The Cellulase Enzyme Activitycontrasting

confidence: 99%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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Background:The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coconut water consumption. In the present work, a new microbial strain was isolated from the coconut shell powder, molecularly identified as Melanoporia sp. CCT 7736 and applied for cellulase production in solid-state fermentation using the green coconut shell powder as substrate. Results:The complete production process was optimized. Fermentation time was only 24 h, and the enzyme produced presented maximal activity at neutral pH (6.5) and 60 °C. The maximal enzyme activity after extraction optimization was 7.5 IU/gds (international units of enzyme activity per gram of dry solid). For the enzyme extraction, the rotation velocity, the extraction time, the temperature, and the solvent volume (buffer) were optimized using response surface methodology (RSM). The best results for the enzyme extraction were obtained at 250 rpm (orbital shaker) at 30 °C using 13.79 mL of a sodium acetate buffer (200 mM) at pH 6.5 after 10 min. Delignification pretreatment was not necessary since this fungus strain was able to degrade the lignin. Conclusions:To the best of our knowledge, this work is the first report of cellulase production by Melanoporia sp. CCT 7736. Good results were obtained without the need for expensive pretreatment usually applied to lignocellulosic residues because the strain was isolated from coconut shell powder, and it is well adapted to this kind of substrate. The enzyme presented maximum activity at neutral pH instead of acidic pH as reported for the majority of industrial cellulases. The use of lignified coconut shell and the optimal pH at neutral values are the main advantages of the enzyme produced by Melanoporia sp. CCT 7736. In addition, the enzyme showed good stability during storage even at the crude broth and without any cryoprotection.

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Section: Effect Of Temperature and Ph On The Cellulase Enzyme Activitycontrasting

confidence: 99%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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“…Optimum temperature was observed at 60 °C, suggesting that this xylanase can be a thermophilic enzyme which can be useful for many biotechnological processes which operate at high temperatures ( Figure 3B). This characteristic confers to this enzyme potential uses in the industry since its action represents a key step for efficient biomass degradation in saccharification processes where high temperatures are aimed (DELABONA et al, 2013). Enzymes with high optimal temperatures are also resistant to detergent agents, to proteolytic enzymes and are stable to a wide range of pHs, increasing their application in different conditions and processes (GOMES et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Wheat bran is also a mostly available waste which comprises about 46% of polysaccharides (glucuronoarabinoxylans, cellulose, mixed linked (1-3)(1-4)-β-D-glucans and starch), proteins (15-22%) and lignin (4-8%) (SOUZA et al, 2012). These substrates have been tested as carbon sources for the production of cellulases and hemicellulases by fungal strains (LIU et al, 2011;DELABONA et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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“…There are many studies in the literature using wheat bran for the production of industrially important enzymes by SSF, however all of them achieved to produce the enzymes with the aid of nutritive supplementary liquids added to the residues (Delabona et al, 2013;Fontana et al, 2005;Meena et al, 2013;Taş kın and Eltem, 2008). The motivation of the current study is the production of PG having reasonably high activities with the use of only wheat bran and distilled water by an Aspergillus sojae mutant strain.…”

Section: Introductionmentioning

confidence: 99%

Valorization of wheat bran for the production of polygalacturonase in SSF of Aspergillus sojae

Demi̇r

Tarı

2014

Industrial Crops and Products

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a b s t r a c tWheat bran, among various agro industrial by products, screened for the production of polygalacturonase (PG) in solid-state fermentation of Aspergillus sojae mutant strain, was found to be the most suitable substrate without the addition of any nutritive or inducing supplement. It was further characterized for its physicochemical composition and particle size distribution. The process conditions that favored the PG production using this substrate were determined as; 10 7 spore/g substrate inoculum concentration, 4 days of fermentation, 37• C of incubation temperature, 62% initial moisture content, water as the moistening agent, 100-250 m particle size of wheat bran, 3 times/day agitation and spore solution as the inoculum type which resulted into maximum PG activity of 535.4 U/g substrate. Overall, this optimization process resulted in 7.3 and 3.9 fold of significant enhancement in the PG activity and productivity, respectively.

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Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization

Cited by 102 publications

References 28 publications

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Valorization of wheat bran for the production of polygalacturonase in SSF of Aspergillus sojae

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