Effect of Inoculation with Preactivated Lactobacillus Buchneri and Urea on Fermentative Profile, Aerobic Stability and Nutritive Value in Corn Silage

Santos, Ana Paula Maia dos; Santos, Edson Mauro; Araújo, Gherman Garcia Leal de; Oliveira, Juliana Silva de; Zanine, Anderson de Moura; Pinho, Ricardo Martins Araújo; Cruz, Gabriel; Ferreira, Daniele de Jesus; Perazzo, Alexandre Fernandes; Pereira, Dimmitre Morant Vieira Gonçalves; Santos, Francisco Naysson de Sousa

doi:10.3390/agriculture10080335

Cited by 11 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Butyric acid, as the main product of Clostridium sp., should not be detectable in well-fermented silage (Kung et al, 2018a). In contrast to the present study, Santos et al (2020) observed the presence of butyric acid in all samples of corn silage (0.03-0.04%) along with a positive effect of activated L. buchneri. The different content of formic acid was observed in silage LAB2 in comparison with C (P < 0.05).…”

Section: The Main Fermentation Parameters Of Corn Silagecontrasting

confidence: 99%

Effect of various additives on the fermentation quality of corn silage

Juráček,

Bíro,

Šimko

et al. 2024

JCEA

View full text Add to dashboard Cite

This study evaluated the effects of biological silage additives with and without chemical salts on the fermentation parameters of whole plant corn silage. The experiment consisted of five treatments: two with lactic acid bacteria (LAB without chemical salts), two with combined additives (LAB + chemical salts), and a control. Firstly, control ( C ) and subsequently facultatively heterofermentative ( A ) and obligately heterofermentative ( B ) LAB (LAB1: L. plantarum A , L. brevis B , E. faecium A ; LAB2: L. plantarum A , L. buchneri B , P. pentosaceus A ) were examined. After that, LABCHSE inclusive of obligately homofermentative ( C ) and facultatively heterofermentative ( A ) LAB (L. plantarum A , L. salivarius C , E. faecium A , P.acidilactici C ) + chemical salts (sodium benzoate, potassium sorbate) + enzymes (cellulase, hemicellulase, pentosanase, amylase) were evaluated. The last variant LABCHS with LAB (L. plantarum A , E. faecium A , P. acidilactici C ) + chemical salt (potassium sorbate) was consisted. After six weeks of storage, the results confirmed that the application of additives can affect the quality of the fermentation process by decreasing lactic acid as a result of heterofermentative bacteria fermentation, the most strongly marked additive based on L. buchneri. The addition of all additives increased the titratable acidity and acetic content, which manifested in a narrowed lactic/acetic acid ratio and an increased pH value because of heterofermentative bacteria fermentation. The addition of additives containing chemical salts affected the fermentation process more favourably compared to the addition of additives without chemical salts, particularly by inhibiting alcohol content.

show abstract

Section: The Main Fermentation Parameters Of Corn Silagecontrasting

confidence: 99%

Effect of various additives on the fermentation quality of corn silage

Juráček,

Bíro,

Šimko

et al. 2024

JCEA

View full text Add to dashboard Cite

show abstract

“…The AA content of the BS group was significantly higher than that of the CK group, which may account for the better aerobic stability of the BS group ( Basso et al, 2012 ; Bai et al, 2022a ). Acetic acid has good antifungal properties and can inhibit the growth of fungi, thereby inhibiting the process of aerobic spoilage of silage ( Santos et al, 2020 ). There was no significant difference in the number of yeasts among treatments, and the number of filamentous fungi was below the detection limit, because the lower pH maintains an acidic environment and inhibits the growth of yeasts and filamentous fungi ( Sun et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…thereby inhibiting the process of aerobic spoilage of silage (Santos et al, 2020). There was no significant difference in the number of yeasts among treatments, and the number of filamentous fungi was below the detection limit, because the lower pH maintains an acidic environment and inhibits the growth of yeasts and filamentous fungi (Sun et al, 2021).…”

Section: Materials Characteristics and Silage Qualitymentioning

confidence: 96%

Effects of Bacillus subtilis or Lentilactobacillus buchneri on aerobic stability, and the microbial community in aerobic exposure of whole plant corn silage

et al. 2023

View full text Add to dashboard Cite

This study aimed to evaluate the effects of Bacillus subtilis or Lentilactobacillus buchneri on the fermentation quality, aerobic stability, and bacterial and fungal communities of whole plant corn silage during aerobic exposure. Whole plant corn was harvested at the wax maturity stage, which chopped to a length of approximately 1 cm, and treated with the following: distilled sterile water control, 2.0 × 105 CFU/g of Lentilactobacillus buchneri (LB) or 2.0 × 105 CFU/g of Bacillus subtilis (BS) for 42 days silage. Then, the samples were exposed to air (23–28°C) after opening and sampled at 0, 18 and 60 h, to investigate fermentation quality, bacterial and fungal communities, and aerobic stability. Inoculation with LB or BS increased the pH value, acetic acid, and ammonia nitrogen content of silage (P < 0.05), but it was still far below the threshold of inferior silage, the yield of ethanol was reduced (P < 0.05), and satisfactory fermentation quality was achieved. With the extension of the aerobic exposure time, inoculation with LB or BS prolonged the aerobic stabilization time of silage, attenuated the trend of pH increase during aerobic exposure, and increased the residues of lactic acid and acetic acid. The bacterial and fungal alpha diversity indices gradually declined, and the relative abundance of Basidiomycota and Kazachstania gradually increased. The relative abundance of Weissella and unclassified_f_Enterobacteria was higher and the relative abundance of Kazachstania was lower after inoculation with BS compared to the CK group. According to the correlation analysis, Bacillus and Kazachstania are bacteria and fungi that are more closely related to aerobic spoilage and inoculation with LB or BS could inhibit spoilage. The FUNGuild predictive analysis indicated that the higher relative abundance of fungal parasite-undefined saprotroph in the LB or BS groups at AS2, may account for its good aerobic stability. In conclusion, silage inoculated with LB or BS had better fermentation quality and improved aerobic stability by effectively inhibiting the microorganisms that induce aerobic spoilage.

show abstract

“…Apart from T-2 toxin, none of the listed mycotoxins in table 1 exceeded the maximum permitted limit set by the European Union. Several authors paid attention to the effect of urea addition (Santos et al, 2021;Fiantok, 2020;Kaewpila et al, 2020;Santos et al, 2020;Zamir et al, 2020;Santos et al, 2018;Kang et al, 2018;Kebede et al, 2018), but monitored the changes in nutritional…”

Section: Resultsmentioning

confidence: 99%

The effect of additives on mycotoxic contamination of maize silages

Kalúzová¹,

Juráček²,

Bíro³

et al. 2022

JCEA

View full text Add to dashboard Cite

Addition of silage additives is commonly concentrated on the improvement of nutritive and fermentative features of silages, but in recent years, mycotoxic contamination is also a feature monitored after the treatment. This study aimed to evaluate the effect of silage additives, in the concrete inoculant consisting of a mixture of Lactobacillus buchneri LN40177, Lactobacillus casei LC329090 and urea, on the concentration of major mycotoxins in maize silages. The ensilage mass was made in three variants, which consisted of maize silage (control variant C), maize silage with the addition of a commercial inoculant (variant A) and maize silage with the addition of urea (variant U). The commercial inoculant was added to the cut mass at a dose of 1 g/t of mass and urea at a dose of 5 kg/t of mass. After application of silage additives, the mass was stored and sealed in silage units. Mycotoxin analysis was performed using Veratox tests with ELISA reader, whereas average samples were prepared according to established protocols. The immuno-enzymatic method revealed that all samples of maize silage showed 100% contamination. The maize silages were characterized by the highest concentration of deoxynivalenol regardless of the treatment. The results confirmed the effect of silage additives on mycotoxic contamination of maize silages. Compared to the control variant, the commercial inoculant had negative increasing effect (P<0.05) on deoxynivalenol, ochratoxins and zearalenone, and a positive decreasing effect (P<0.05) on T-2 toxin and fumonisins. The urea addition resulted in significant reduction of T-2 toxin and increase of zearalenone.

show abstract

Effect of Inoculation with Preactivated Lactobacillus Buchneri and Urea on Fermentative Profile, Aerobic Stability and Nutritive Value in Corn Silage

Cited by 11 publications

References 33 publications

Effect of various additives on the fermentation quality of corn silage

Effect of various additives on the fermentation quality of corn silage

Effects of Bacillus subtilis or Lentilactobacillus buchneri on aerobic stability, and the microbial community in aerobic exposure of whole plant corn silage

The effect of additives on mycotoxic contamination of maize silages

Contact Info

Product

Resources

About