Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Abstract

Success of embryo transfer is determined by availability of a plenty of amount of embryo stock. To make embryo stock remain in good quality, it is necessary to have an easy, cheap, simple and effective storage method. One of the storage methods is vitrification.Success of vitrification method is still disrupted due to decreased embryo quality post warming. Decreased embryo viability influences high implantation rate and gestation. Low implantation rate will result in low gestation rate. Therefore, study is needed to optimize vitrification medium so that it will be able to optimize cryoprotectant role to protect embryo due to temperature stressor in vitrification method.Administering Insulin Transferrin Selenium on vitrification medium is able to bind free radicals caused by temperature stressor due to frozen Insulin Transferrin Selenium which is a complex protein that is able to stimulate cell growth, prevent cell damage due to anti oxidant role in it so that it is able to maintain embryo viability post thowing. Insulin Transferrin Selenium is able to increase quality and viability of blastocyst resulted from in vitro culture.The study aims to prove Insulin Transferrin Selenium supplementation on vitrification medium is able to increase viability of rat embryo post warming. Steps of the research cover oocyte collection, embryo vitrification, warming, and in vitro culture. In vitro culture yields morula embryo with excellent quality and fits to be frozen using vitrification method.Insulin Transferin Selenium administration of 5,10 and 15 g/ml is able to increase embryo viability up to 100 %. Frozen embryo post warming and recultured for 5 hours , viability of morula embryo ofthe treatment group administered by Insulin Transferrin Selenium reached 73.4 % -83.4% whereas control group without being administered by InsulinTransferrin Selenium reached only 64.7 %. The research concludes that Insulin Transferrin Selenium.administration is able to increase viability of rat embryo at morula stage.