Effect of Interval between Human Chorionic Gonadotropin Priming and Ovum Pick-up on the Euploid Probabilities of Blastocyst

Abstract

This retrospective study attempts to elucidate the relevance of the interval between human chorionic gonadotropin priming and oocyte pick-up (hCG-OPU) to the euploidy probability of biopsied blastocysts in preimplantation genetic tests for aneuploidy (PGT-A) cycles. A total of 1889 blastocysts from 511 patients undergoing PGT- A cycles were used. An analysis of generalized estimating equations (GEE) was used to identify whether the hCG–OPU interval is associated with euploidy probabilities of blastocysts. Accordingly, maternal age (OR: 0.925, 95% CI: 0.903–0.948, p < 0.001) and the hCG–OPU interval (OR: 1.138, 95% CI: 1.028–1.260, p = 0.013) were the two significant factors associated with the euploidy probabilities. The Cochran-Armitage trend test demonstrated that the blastocyst euploidy percentage increased progressively with the increasing hCG-OPU interval in normal responders (p = 0.006) and advanced maternal age (age ≥38 years; p = 0.020) groups. In normal responders, the euploidy rate was highest in the 38–39 h interval (43.1%, 47/109). In contrast, the euploidy rate was lowest in the 34–35 h interval (28.7%, 29/105). In conclusion, the present study demonstrated that at an hCG-OPU interval between 34–39 h, the longer the hCG-OPU interval, the higher the probability of euploidy for blastocysts.

Time interval between hCG administration and oocyte retrieval and ART outcomes: an updated systematic review and meta-analysis

“…Furthermore, oocyte cytoplasmic immaturity was associated with metaphase plate anomalies and aneuploidies [ 43 ]. It was also observed that the euploidy rate was highest in the 38–39 h interval and lowest in the 34–35 h interval [ 5 ]. Longer hCG-OPU interval increases the production of oocytes with fully expanded cumulus, which might reflect oocyte maturation.…”

“…This interval is vital as some indispensable processes, including luteinization initiation, cumulus cell expansion, and oocyte meiosis resumption, should be well-established before aspiration [ 4 ]. It has been shown that, in addition to maternal age, the hCG-OPU interval was a significant factor associated with euploidy probabilities [ 5 ]. Therefore, this interval should be carefully managed to obtain the maximum number of eligible mature oocytes while avoiding spontaneous ovulation.…”

Blastocyst Morphology Based on Uniform Time-Point Assessments is Correlated With Mosaic Levels in Embryos

“…Patients who were assigned the GnRH antagonist protocol received the gonadotropin regimen, same as that in the agonist long protocol, and additional cetrorelix acetate (Cetrotide; Merck Serono, Geneva, Switzerland) injections (0.25 mg/day) were given subcutaneously before hCG injection. Oocyte maturation was triggered through hCG administration, and ultrasound-guided ovum retrieval was performed approximately 36 h after hCG injection ( Lee et al, 2020a ).…”