Effect of iodinated radiographic contrast media on radioimmunoassay for measuring thyroid hormones

Thyroxine Quantification by Using Plasmonic Nanoparticles as SERS Substrates

“…For comparative purposes, an area of the wafer of the same size (2.7 µm × 3.6 µm) is shown in both panels. The area shown is smaller than the laser spot area (12.56 µm 2 ); Figure S4: Image of the thyroxine molecule inside a rectangular prism; Figure S5: Near electric field intensity image of an Au NP of 50 nm of diameter and plot of the mean |E| 4 with increasing distance from the Au NP surface; Figure S6: SEM micrograph at a magnification of ×15,000 of the SERS substrate of (a) Au NPs and (b) Ag NPs; Table S1: Molecule concentration of T4, the total number of molecules in each T4 solution concentration, number of molecule-layers on the Si wafer, and the total thickness of the multi-layer array, and N Raman value; Table S2: Values used to estimate the total metal area occupied by the NP that is under the laser spot.…”

“…Additionally, focusing attention on the T4 hormone (free or bound), it is important to track triiodothyronine (T3)-both bound and free forms-the thyroid stimulating hormone (TSH, also named thyrotropin), and the thyroid releasing hormone (TRH), to make a proper diagnosis of thyroid gland function. As reference values, we can indicate normal, hypothyroid, and hyperthyroid hormone conditions at 1.2 ng/mL, 0.2 ng/mL, and 2.2 ng/mL, respectively, for the T3 concentration, and 70 ng/mL, 30 ng/mL, and 140 ng/mL for the T4 concentration, respectively [4]. Normal ranges for thyroid tests may vary slightly among different laboratories.…”

“…Using these methods, it has been possible to detect FT3, FT4, and TSH at normal and hypothyroidism levels [4,[6][7][8][9]. However, some drawbacks of these tests are that patients may receive intravenous iodine as a contrast medium before the serum thyroxine test, long assay and sample preparation times, expensive instruments, and the employment of radioactive tracers.…”