Effect of ionic stress on apoptosis and the expression of TRPM2 in human olfactory neuroepithelial-derived progenitors

Gao, Yonglin; Lei, Zhenmin; Lu, Chengliang; Roisen, Fred J.; El‐Mallakh, Rif S.

doi:10.3109/15622975.2010.507784

Cited by 15 publications

(9 citation statements)

References 65 publications

(77 reference statements)

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“…Cellular effects of lithium, a first-line bipolar disorder treatment, have also been explored in ON-derived cells, revealing amelioration by lithium of ionic stress-induced apoptosis and TRPM2 overexpression. 141 In major depressive disorder, there is evidence that ON-derived cells display decreased nuclear translocation of the glucocortocoid receptor, GR, in response to glucocorticoids relative to controls. 142 This diminished receptor translocation was accompanied by greater cytoplasmic association of GR with the immunophillin FKBP51.…”

Section: The Use Of Om-derived Cells In Psychiatry and Neuroscience Rmentioning

confidence: 99%

Translational potential of olfactory mucosa for the study of neuropsychiatric illness

Borgmann‐Winter

Sinclair

et al. 2015

Transl Psychiatry

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The olfactory mucosa (OM) is a unique source of regenerative neural tissue that is readily obtainable from living human subjects and thus affords opportunities for the study of psychiatric illnesses. OM tissues can be used, either as ex vivo OM tissue or in vitro OM-derived neural cells, to explore parameters that have been difficult to assess in the brain of living individuals with psychiatric illness. As OM tissues are distinct from brain tissues, an understanding of the neurobiology of the OM is needed to relate findings in these tissues to those of the brain as well as to design and interpret ex vivo or in vitro OM studies. To that end, we discuss the molecular, cellular and functional characteristics of cell types within the olfactory mucosa, describe the organization of the OM and highlight its role in the olfactory neurocircuitry. In addition, we discuss various approaches to in vitro culture of OM-derived cells and their characterization, focusing on the extent to which they reflect the in vivo neurobiology of the OM. Finally, we review studies of ex vivo OM tissues and in vitro OM-derived cells from individuals with psychiatric, neurodegenerative and neurodevelopmental disorders. In particular, we discuss the concordance of this work with postmortem brain studies and highlight possible future approaches, which may offer distinct strengths in comparison to in vitro paradigms based on genomic reprogramming.

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Section: The Use Of Om-derived Cells In Psychiatry and Neuroscience Rmentioning

confidence: 99%

Translational potential of olfactory mucosa for the study of neuropsychiatric illness

Borgmann‐Winter

Sinclair

et al. 2015

Transl Psychiatry

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“…Utilize neuronal cells obtained from patients and controls (e.g., induced pluripotent cells [104] or olfactory neuroepithelial cells [105]) in order to determine the differential effect (if any) in the response of neural tissue derived from patients with BD and non-psychiatric controls. Early exploratory evidence from immortalized lymphoblasts [106], induced pluripotent cells, and olfactory neuroepithelial progenitors [107][108][109][110][111], suggests that such differences exist.…”

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confidence: 99%

Endogenous Cardiac Steroids in Bipolar Disorder: State of the Art

El‐Mallakh

Sampath²,

Horesh³

et al. 2022

IJMS

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Bipolar disorder (BD) is a severe psychiatric illness with a poor prognosis and problematic, suboptimal, treatments. Treatments, borne of an understanding of the pathoetiologic mechanisms, need to be developed in order to improve outcomes. Dysregulation of cationic homeostasis is the most reproducible aspect of BD pathophysiology. Correction of ionic balance is the universal mechanism of action of all mood stabilizing medications. Endogenous sodium pump modulators (collectively known as endogenous cardiac steroids, ECS) are steroids which are synthesized in and released from the adrenal gland and brain. These compounds, by activating or inhibiting Na+, K+-ATPase activity and activating intracellular signaling cascades, have numerous effects on cell survival, vascular tone homeostasis, inflammation, and neuronal activity. For the past twenty years we have addressed the hypothesis that the Na+, K+-ATPase-ECS system may be involved in the etiology of BD. This is a focused review that presents a comprehensive model pertaining to the role of ECS in the etiology of BD. We propose that alterations in ECS metabolism in the brain cause numerous biochemical changes that underlie brain dysfunction and mood symptoms. This is based on both animal models and translational human results. There are data that demonstrate that excess ECS induce abnormal mood and activity in animals, while a specific removal of ECS with antibodies normalizes mood. There are also data indicating that circulating levels of ECS are lower in manic individuals, and that patients with BD are unable to upregulate synthesis of ECS under conditions that increase their elaboration in non-psychiatric controls. There is strong evidence for the involvement of ion dysregulation and ECS function in bipolar illness. Additional research is required to fully characterize these abnormalities and define future clinical directions.

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“…Previous studies have shown that MNS inhibits the cell proliferation as a consequence of arresting the cell-cycle progression, leading to apoptotic cell death in various cells (Park et al, 2002a(Park et al, , 2002b(Park et al, , 2003a(Park et al, , 2003b. Furthermore, MNS has recently been shown to cause oxidative damage to the cells, resulting in the apoptotic cell death in olfactory neuroepithelial-derived progenitors and prostate cancer cells (Gao et al, 2010;Ketola et al, 2010). In contrast, the findings presented in this paper suggest that PTX can cause the cytotoxic action through a different mechanism from that of MNS, and provide evidence for supporting the nonoxidative and necrotic action of PTX on PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

Palytoxin causes nonoxidative necrotic damage to PC12 cells in culture

Sagara

Nishibori

Itoh

et al. 2011

J of Applied Toxicology

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Palytoxin (PTX) is a potent marine toxin that causies serious damage to various tissues and organs. It has been reported to affect the transport of cations across the plasma membranes, which is commonly recognized as being the principal mechanism of its highly toxic action on mammals, including humans. However, although some marine toxins have been shown to cause toxic effects on the nervous system by interfering with the transmission of nerve impulses, the effect of PTX on neuronal cells has not yet been fully elucidated. Therefore, the toxic action of PTX on PC12 cells was examined as an in vitro model experiment to elucidate the neurotoxic properties of this toxin, and PTX was shown to reduce the viability of PC12 cells in a concentration-dependent manner. The cytotoxic action of PTX was not significantly altered by the presence of the antioxidant N-acetylcysteine and reduced-form glutathione in the cultures. Fluorescence staining of the cells and the electrophoretic analysis of genomic DNA showed that PTX failed to cause chromatin condensation and DNA fragmentation within the cells. On the other hand, the exposure to PTX caused positive staining of the cytoplasmic space of the cells with propidium iodide and the release of lactate dehydrogenase into the culture medium. Based on these observations, PTX is considered to cause cell death as a consequence of disrupting the plasma membranes, thus causing nonoxidative necrotic damage to PC12 cells.

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Effect of ionic stress on apoptosis and the expression of TRPM2 in human olfactory neuroepithelial-derived progenitors

Abstract: Our findings suggest that the elevation of [Na(+)](in) and [Ca(2+)](in) induced ONP apoptosis and altered the expression of TRPM2. Lithium pretreatment attenuated the apoptosis induced by ionic stress.

Cited by 15 publications

References 65 publications

Translational potential of olfactory mucosa for the study of neuropsychiatric illness

Translational potential of olfactory mucosa for the study of neuropsychiatric illness

Endogenous Cardiac Steroids in Bipolar Disorder: State of the Art

Palytoxin causes nonoxidative necrotic damage to PC12 cells in culture

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