Effect of IPs, cAMP, and cGMP on the hPL and hCG secretion from human term placenta

Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells

“…The PKA-mediated cAMP signaling pathway is required for hCG synthesis (Chou, 1980;Nulsen et al, 1998;Lambot et al, 2005). Accordingly, the significant increase of hCG production was accompanied by the up-regulation of both hCGa and hCGb mRNAs after treatment with a PKA-selective cAMP analog (Phe).…”

“…It was reported that the enhancement of intracellular cAMP levels via treatment with various cAMP analogs (Chou, 1980;Lambot et al, 2005) and forskolin (FSK), which is an activator of adenylate cyclase (Nulsen et al, 1998), results in activation of protein kinase A (PKA) and promotes functional differentiation, such as the production of hCG and progesterone and syncytialization in primary isolated trophoblasts and in the trophoblast-derived human choriocarcinoma cell line BeWo.…”